2-hydroxypropyl-CoM lyase

2-hydroxypropyl-CoM lyase
Identifiers
EC number	4.4.1.23
CAS number	244301-07-3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

2-hydroxypropyl-CoM lyase (EC 4.4.1.23, epoxyalkane:coenzyme M transferase, epoxyalkane:CoM transferase, epoxyalkane:2-mercaptoethanesulfonate transferase, coenzyme M-epoxyalkane ligase, epoxyalkyl:CoM transferase, epoxypropane:coenzyme M transferase, epoxypropyl:CoM transferase, EaCoMT, 2-hydroxypropyl-CoM:2-mercaptoethanesulfonate lyase (epoxyalkane-ring-forming), (R)-2-hydroxypropyl-CoM 2-mercaptoethanesulfonate lyase (cyclizing, (R)-1,2-epoxypropane-forming)) is an enzyme with systematic name (R)-(or (S)-)2-hydroxypropyl-CoM:2-mercaptoethanesulfonate lyase (epoxyalkane-ring-forming).^[1][2][3] This enzyme catalyses the following chemical reaction

(1) (R)-2-hydroxypropyl-CoM $\rightleftharpoons$ (R)-1,2-epoxypropane + HS-CoM

(2) (S)-2-hydroxypropyl-CoM $\rightleftharpoons$ (S)-1,2-epoxypropane + HS-CoM

This enzyme requires zinc.

References

1. Allen, J.R., Clark, D.D., Krum, J.G. and Ensign, S.A. (1999). "A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation". Proc. Natl. Acad. Sci. USA 96: 8432–8437. doi:10.1073/pnas.96.15.8432. PMC 17533. PMID 10411892.
2. Krum, J.G., Ellsworth, H., Sargeant, R.R., Rich, G. and Ensign, S.A. (2002). "Kinetic and microcalorimetric analysis of substrate and cofactor interactions in epoxyalkane:CoM transferase, a zinc-dependent epoxidase". Biochemistry 41: 5005–5014. doi:10.1021/bi0255221. PMID 11939797.
3. Coleman, N.V. and Spain, J.C. (2003). "Epoxyalkane: coenzyme M transferase in the ethene and vinyl chloride biodegradation pathways of Mycobacterium strain JS60". J. Bacteriol. 185: 5536–5545. doi:10.1128/jb.185.18.5536-5545.2003. PMID 12949106.

External links

• 2-hydroxypropyl-CoM lyase at the US National Library of Medicine Medical Subject Headings (MeSH)