2-oxopropyl-CoM reductase (carboxylating)

2-oxopropyl-CoM reductase (carboxylating)
Identifiers
EC number	1.8.1.5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a 2-oxopropyl-CoM reductase (carboxylating) (EC 1.8.1.5) is an enzyme that catalyzes the chemical reaction

2-mercaptoethanesulfonate + acetoacetate + NADP⁺ $\rightleftharpoons$ 2-(2-oxopropylthio)ethanesulfonate + CO₂ + NADPH

The 3 substrates of this enzyme are 2-mercaptoethanesulfonate, acetoacetate, and NADP⁺, whereas its 3 products are 2-(2-oxopropylthio)ethanesulfonate, CO₂, and NADPH.

This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-mercaptoethanesulfonate,acetoacetate:NADP+ oxidoreductase (decarboxylating). Other names in common use include NADPH:2-(2-ketopropylthio)ethanesulfonate, oxidoreductase/carboxylase, and NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2C3C and 2C3D.

References

• Allen JR, Clark DD, Krum JG, Ensign SA (1999). "A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation". Proc. Natl. Acad. Sci. U.S.A. 96 (15): 8432–7. doi:10.1073/pnas.96.15.8432. PMC 17533. PMID 10411892.
• Clark DD, Allen JR, Ensign SA (2000). "Characterization of five catalytic activities associated with the NADPH:2-ketopropyl-coenzyme M [2-(2-ketopropylthio)ethanesulfonate] oxidoreductase/carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system". Biochemistry. 39 (6): 1294–304. doi:10.1021/bi992282p. PMID 10684609.