3-chloro-D-alanine dehydrochlorinase

3-chloro-D-alanine dehydrochlorinase
Identifiers
EC number	4.5.1.2
CAS number	78990-65-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a 3-chloro-D-alanine dehydrochlorinase (EC 4.5.1.2) is an enzyme that catalyzes the chemical reaction

3-chloro-D-alanine + H₂O $\rightleftharpoons$ pyruvate + chloride + NH₃

Thus, the two substrates of this enzyme are 3-chloro-D-alanine and H₂O, whereas its 3 products are pyruvate, chloride, and NH₃.

This enzyme belongs to the family of lyases, specifically the class of carbon-halide lyases. The systematic name of this enzyme class is 3-chloro-D-alanine chloride-lyase (deaminating; pyruvate-forming). Other names in common use include beta-chloro-D-alanine dehydrochlorinase, and 3-chloro-D-alanine chloride-lyase (deaminating). It employs one cofactor, pyridoxal phosphate.

References

• Nagasawa T, Ishii T, Yamada H (1988). "Physiological comparison of D-cysteine desulfhydrase of Escherichia coli with 3-chloro-D-alanine dehydrochlorinase of Pseudomonas putida CR 1-1". Arch. Microbiol. 149 (5): 413–6. doi:10.1007/BF00425580. PMID 3132906.
• Yamada H, Nagasawa T, Ohkishi H, Kawakami B, Tani Y (1981). "Synthesis of D-cysteine from 3-chloro-D-alanine and hydrogen sulfide by 3-chloro-D-alanine hydrogen chloride-lyase (deaminating) of Pseudomonas putida". Biochem. Biophys. Res. Commun. 100 (3): 1104–10. doi:10.1016/0006-291X(81)91937-9. PMID 6791643.