4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
Identifiers
EC number	3.7.1.17
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase (EC 3.7.1.17, tesD (gene), hsaD (gene)) is an enzyme with systematic name 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase ( (2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming).^[1][2][3][4] This enzyme catalyses the following chemical reaction

(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H₂O $\rightleftharpoons$ 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate

The enzyme is involved in the bacterial degradation of the steroid ring structure.

References

1. Horinouchi, M., Hayashi, T., Koshino, H., Kurita, T. and Kudo, T. (2005). "Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441". Appl. Environ. Microbiol. 71: 5275–5281. doi:10.1128/aem.71.9.5275-5281.2005. PMID 16151114.
2. Van der Geize, R., Yam, K., Heuser, T., Wilbrink, M.H., Hara, H., Anderton, M.C., Sim, E., Dijkhuizen, L., Davies, J.E., Mohn, W.W. and Eltis, L.D. (2007). "A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages". Proc. Natl. Acad. Sci. USA 104: 1947–1952. doi:10.1073/pnas.0605728104. PMID 17264217.
3. Lack, N., Lowe, E.D., Liu, J., Eltis, L.D., Noble, M.E., Sim, E. and Westwood, I.M. (2008). "Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis". Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64: 2–7. doi:10.1107/s1744309107065931. PMID 18097091.
4. Lack, N.A., Yam, K.C., Lowe, E.D., Horsman, G.P., Owen, R.L., Sim, E. and Eltis, L.D. (2010). "Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism". J. Biol. Chem. 285: 434–443. doi:10.1074/jbc.m109.058081. PMID 19875455.

External links

• 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase at the US National Library of Medicine Medical Subject Headings (MeSH)