6-Carboxyfluorescein

6-Carboxyfluorescein
Names
Other names
6-FAM
Identifiers
3301-79-9 YesY
ChEBI CHEBI:39073 YesY
ChemSpider 69262 YesY
Jmol 3D model Interactive image
Properties
C21H12O7
Molar mass 376.32 g·mol−1
Hazards
GHS pictograms
GHS signal word Warning
H315, H319, H335
P261, P305+351+338
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
YesY verify (what is YesYN ?)
Infobox references

6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents. It is used in the sequencing of nucleic acids and in the labelling nucleotides.

Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.

The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading.[1] It can be incorporated into liposomes, and allow for the tracking of liposomes as they pass through the body. In addition, carboxyfluorescein has been used to track division of cells.[2]

Popular derivatives for cell tracing purposes are Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and Carboxyfluorescein succinimidyl ester (CFSE).

See also

References

  1. Molecular Imaging Products Company (2005-08-26). "5-(and-6)-Carboxyfluorescein (5-(and-6)- FAM,mixed isomer) 100mg". Retrieved 2006-08-26.
  2. Parish, Christopher (December 1999). "Fluorescent dyes for lymphocyte migration and proliferation studies". Immunology and Cell Biology. Blackwell Synergy. Retrieved 2006-08-26.
This article is issued from Wikipedia - version of the Saturday, April 16, 2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.