AHNAK

AHNAK nucleoprotein
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4DRW​, 4FTG​, 4HRG​
Identifiers
Symbols	AHNAK ; AHNAKRS
External IDs	OMIM: 103390 MGI: 1316648 HomoloGene: 67425 GeneCards: AHNAK Gene
Gene ontology Molecular function • protein binding • S100 protein binding • poly(A) RNA binding • structural molecule activity conferring elasticity Cellular component • nucleus • cytoplasm • lysosomal membrane • cytosol • plasma membrane • focal adhesion • actin cytoskeleton • membrane • T-tubule • vesicle • sarcolemma • costamere • cell-cell contact zone • membrane raft • extracellular exosome Biological process • regulation of RNA splicing • protein oligomerization • regulation of voltage-gated calcium channel activity Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	79026	66395
Ensembl	ENSG00000124942	ENSMUSG00000069833
UniProt	Q09666	E9Q616
RefSeq (mRNA)	NM_001620	NM_001039959
RefSeq (protein)	NP_001611	NP_001035048
Location (UCSC)	Chr 11: 62.43 – 62.56 Mb	Chr 19: 8.99 – 9.08 Mb
PubMed search

Neuroblast differentiation-associated protein AHNAK, also known as desmoyokin, is a protein that in humans is encoded by the AHNAK gene.^[1][2][3] AHNAK was originally identified in 1989 (in bovine muzzle epidermal cells) and named desmoyokin due to its localization pattern (that resembled a yoke) in the desmosomal plaque.^[4] AHNAK has been shown to be essential for pseudopod protrusion and cell migration. ^[5]

Interactions

AHNAK has been shown to interact with S100B.^[6]

References

1. James MR, Richard CW 3rd, Schott JJ, Yousry C, Clark K, Bell J, Terwilliger JD, Hazan J, Dubay C, Vignal A; et al. (Jan 1995). "A radiation hybrid map of 506 STS markers spanning human chromosome 11". Nat Genet 8 (1): 70–6. doi:10.1038/ng0994-70. PMID 7987395.
2. Hohaus A, Person V, Behlke J, Schaper J, Morano I, Haase H (Aug 2002). "The carboxyl-terminal region of ahnak provides a link between cardiac L-type Ca2+ channels and the actin-based cytoskeleton". FASEB J 16 (10): 1205–16. doi:10.1096/fj.01-0855com. PMID 12153988.
3. "Entrez Gene: AHNAK AHNAK nucleoprotein (desmoyokin)".
4. Hieda Y, Tsukita S, Tsukita S (Oct 1989). "A new high molecular mass protein showing unique localization in desmosomal plaque.". J Cell Biol. 109 (4): 1511–1518. doi:10.1083/jcb.109.4.1511. PMC 2115823. PMID 2677021.
5. Shankar J, Messenberg A, Chan J, Underhill TM, Foster LJ, Nabi IR (May 2010). "Pseudopodial actin dynamics control epithelial-mesenchymal transition in metastatic cancer cells.". Cancer Res. 70 (9): 3780–3790. doi:10.1158/0008-5472.CAN-09-4439. PMID 20388789.
6. Gentil, B J; Delphin C; Mbele G O; Deloulme J C; Ferro M; Garin J; Baudier J (Jun 2001). "The giant protein AHNAK is a specific target for the calcium- and zinc-binding S100B protein: potential implications for Ca2+ homeostasis regulation by S100B". J. Biol. Chem. (United States) 276 (26): 23253–61. doi:10.1074/jbc.M010655200. ISSN 0021-9258. PMID 11312263.

Further reading

• Shtivelman E, Cohen FE, Bishop JM (1992). "A human gene (AHNAK) encoding an unusually large protein with a 1.2-microns polyionic rod structure.". Proc. Natl. Acad. Sci. U.S.A. 89 (12): 5472–6. doi:10.1073/pnas.89.12.5472. PMC 49314. PMID 1608957.
• Kudoh J, Wang Y, Minoshima S; et al. (1995). "Localization of the human AHNAK/desmoyokin gene (AHNAK) to chromosome band 11q12 by somatic cell hybrid analysis and fluorescence in situ hybridization.". Cytogenet. Cell Genet. 70 (3-4): 218–20. doi:10.1159/000134037. PMID 7789175.
• Dias Neto E, Correa RG, Verjovski-Almeida S; et al. (2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags.". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3491–6. doi:10.1073/pnas.97.7.3491. PMC 16267. PMID 10737800.
• Gentil BJ, Delphin C, Mbele GO; et al. (2001). "The giant protein AHNAK is a specific target for the calcium- and zinc-binding S100B protein: potential implications for Ca2+ homeostasis regulation by S100B.". J. Biol. Chem. 276 (26): 23253–61. doi:10.1074/jbc.M010655200. PMID 11312263.
• Sussman J, Stokoe D, Ossina N, Shtivelman E (2001). "Protein kinase B phosphorylates AHNAK and regulates its subcellular localization.". J. Cell Biol. 154 (5): 1019–30. doi:10.1083/jcb.200105121. PMC 2196192. PMID 11535620.
• Wernyj RP, Ewing CM, Isaacs WB (2004). "Multiple antibodies to titin immunoreact with AHNAK and localize to the mitotic spindle machinery.". Cell Motil. Cytoskeleton 50 (2): 101–13. doi:10.1002/cm.1044. PMID 11746675.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Straub BK, Boda J, Kuhn C; et al. (2004). "A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells.". J. Cell. Sci. 116 (Pt 24): 4985–95. doi:10.1242/jcs.00815. PMID 14625392.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Haase H, Pagel I, Khalina Y; et al. (2004). "The carboxyl-terminal ahnak domain induces actin bundling and stabilizes muscle contraction.". FASEB J. 18 (7): 839–41. doi:10.1096/fj.03-0446fje. PMID 15001564.
• Lee IH, You JO, Ha KS; et al. (2004). "AHNAK-mediated activation of phospholipase C-gamma1 through protein kinase C.". J. Biol. Chem. 279 (25): 26645–53. doi:10.1074/jbc.M311525200. PMID 15033986.
• Stiff T, Shtivelman E, Jeggo P, Kysela B (2004). "AHNAK interacts with the DNA ligase IV-XRCC4 complex and stimulates DNA ligase IV-mediated double-stranded ligation.". DNA Repair (Amst.) 3 (3): 245–56. doi:10.1016/j.dnarep.2003.11.001. PMID 15177040.
• Ahn J, Chung KS, Kim DU; et al. (2005). "Systematic identification of hepatocellular proteins interacting with NS5A of the hepatitis C virus.". J. Biochem. Mol. Biol. 37 (6): 741–8. PMID 15607035.
• Haase H, Alvarez J, Petzhold D; et al. (2006). "Ahnak is critical for cardiac Ca(V)1.2 calcium channel function and its beta-adrenergic regulation.". FASEB J. 19 (14): 1969–77. doi:10.1096/fj.05-3997com. PMID 16319140.
• Tompkins V, Hagen J, Zediak VP, Quelle DE (2006). "Identification of novel ARF binding proteins by two-hybrid screening.". Cell Cycle 5 (6): 641–6. doi:10.4161/cc.5.6.2560. PMID 16582619.
• De Seranno S, Benaud C, Assard N; et al. (2007). "Identification of an AHNAK binding motif specific for the Annexin2/S100A10 tetramer.". J. Biol. Chem. 281 (46): 35030–8. doi:10.1074/jbc.M606545200. PMID 16984913.