AKAP5

A kinase (PRKA) anchor protein 5
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2H9R​, 3LL8​
Identifiers
Symbols	AKAP5 ; AKAP75; AKAP79; H21
External IDs	OMIM: 604688 MGI: 2685104 HomoloGene: 15854 GeneCards: AKAP5 Gene
Gene ontology Molecular function • G-protein coupled receptor binding • actin binding • protein binding • calmodulin binding • adenylate cyclase binding • protein kinase binding • protein domain specific binding • protein phosphatase 2B binding • protein complex binding • protein kinase A regulatory subunit binding • cadherin binding • GABA receptor binding • protein kinase A binding Cellular component • cytosol • cytoskeleton • plasma membrane • postsynaptic density • basolateral plasma membrane • filopodium membrane • asymmetric synapse • dendritic spine membrane • neuronal cell body • dendritic spine • dendritic shaft • protein complex • perinuclear region of cytoplasm Biological process • positive regulation of protein phosphorylation • energy reserve metabolic process • protein targeting • signal transduction • negative regulation of adenylate cyclase activity • synaptic transmission • positive regulation of calcium ion transport into cytosol • positive regulation of protein kinase A signaling • response to muscle activity • spinal cord development • hippocampus development • positive regulation of cAMP biosynthetic process • amylase secretion • negative regulation of potassium ion transport • cellular protein complex disassembly • small molecule metabolic process • positive regulation of adenylate cyclase activity • positive regulation of dendrite morphogenesis • regulation of insulin secretion • positive regulation of NFAT protein import into nucleus • response to electrical stimulus • maternal process involved in female pregnancy • cellular response to organonitrogen compound Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9495	238276
Ensembl	ENSG00000179841	ENSMUSG00000021057
UniProt	P24588	D3YVF0
RefSeq (mRNA)	NM_004857	NM_001101471
RefSeq (protein)	NP_004848	NP_001094941
Location (UCSC)	Chr 14: 64.47 – 64.47 Mb	Chr 12: 76.32 – 76.33 Mb
PubMed search

A-kinase anchor protein 5 is a protein that in humans is encoded by the AKAP5 gene.^[1][2][3]

Function

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene is intronless and encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin 2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT.^[3]

Interactions

AKAP5 has been shown to interact with:

• Calcineurin^[4] and
• GABRB3.^[5]

References

1. Carr DW, Stofko-Hahn RE, Fraser ID, Cone RD, Scott JD (Sep 1992). "Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79". J Biol Chem 267 (24): 16816–23. PMID 1512224.
2. Carr DW, Hausken ZE, Fraser ID, Stofko-Hahn RE, Scott JD (Aug 1992). "Association of the type II cAMP-dependent protein kinase with a human thyroid RII-anchoring protein. Cloning and characterization of the RII-binding domain". J Biol Chem 267 (19): 13376–82. PMID 1618839.
3. "Entrez Gene: AKAP5 A kinase (PRKA) anchor protein 5".
4. Kashishian A, Howard M, Loh C, Gallatin WM, Hoekstra MF, Lai Y (Oct 1998). "AKAP79 inhibits calcineurin through a site distinct from the immunophilin-binding region". J. Biol. Chem. 273 (42): 27412–9. doi:10.1074/jbc.273.42.27412. PMID 9765270.
5. Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (Jan 2003). "A-kinase anchoring protein 79/150 facilitates the phosphorylation of GABA(A) receptors by cAMP-dependent protein kinase via selective interaction with receptor beta subunits". Mol. Cell. Neurosci. 22 (1): 87–97. doi:10.1016/s1044-7431(02)00017-9. PMID 12595241.

Further reading

• Lester LB, Scott JD (1997). "Anchoring and scaffold proteins for kinases and phosphatases". Recent Prog. Horm. Res. 52: 409–29; discussion 429–30. PMID 9238861.
• Michel JJ, Scott JD (2002). "AKAP mediated signal transduction". Annu. Rev. Pharmacol. Toxicol. 42: 235–57. doi:10.1146/annurev.pharmtox.42.083101.135801. PMID 11807172.
• Hirsch AH, Glantz SB, Li Y; et al. (1992). "Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of cAMP-dependent protein kinase II beta". J. Biol. Chem. 267 (4): 2131–4. PMID 1733921.
• Carr DW, Stofko-Hahn RE, Fraser ID; et al. (1991). "Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif". J. Biol. Chem. 266 (22): 14188–92. PMID 1860836.
• Coghlan VM, Perrino BA, Howard M; et al. (1995). "Association of protein kinase A and protein phosphatase 2B with a common anchoring protein". Science 267 (5194): 108–11. doi:10.1126/science.7528941. PMID 7528941.
• Glantz SB, Li Y, Rubin CS (1993). "Characterization of distinct tethering and intracellular targeting domains in AKAP75, a protein that links cAMP-dependent protein kinase II beta to the cytoskeleton". J. Biol. Chem. 268 (17): 12796–804. PMID 8509414.
• Faux MC, Scott JD (1997). "Regulation of the AKAP79-protein kinase C interaction by Ca2+/Calmodulin". J. Biol. Chem. 272 (27): 17038–44. doi:10.1074/jbc.272.27.17038. PMID 9202019.
• Kashishian A, Howard M, Loh C; et al. (1998). "AKAP79 inhibits calcineurin through a site distinct from the immunophilin-binding region". J. Biol. Chem. 273 (42): 27412–9. doi:10.1074/jbc.273.42.27412. PMID 9765270.
• Paolillo M, Feliciello A, Porcellini A; et al. (1999). "The type and the localization of cAMP-dependent protein kinase regulate transmission of cAMP signals to the nucleus in cortical and cerebellar granule cells". J. Biol. Chem. 274 (10): 6546–52. doi:10.1074/jbc.274.10.6546. PMID 10037748.
• Dodge KL, Carr DW, Sanborn BM (1999). "Protein kinase A anchoring to the myometrial plasma membrane is required for cyclic adenosine 3',5'-monophosphate regulation of phosphatidylinositide turnover". Endocrinology 140 (11): 5165–70. doi:10.1210/en.140.11.5165. PMID 10537145.
• Fraser ID, Cong M, Kim J; et al. (2000). "Assembly of an A kinase-anchoring protein-beta(2)-adrenergic receptor complex facilitates receptor phosphorylation and signaling". Curr. Biol. 10 (7): 409–12. doi:10.1016/S0960-9822(00)00419-X. PMID 10753752.
• Sík A, Gulácsi A, Lai Y; et al. (2000). "Localization of the A kinase anchoring protein AKAP79 in the human hippocampus". Eur. J. Neurosci. 12 (4): 1155–64. doi:10.1046/j.1460-9568.2000.00002.x. PMID 10762347.
• Colledge M, Dean RA, Scott GK; et al. (2000). "Targeting of PKA to glutamate receptors through a MAGUK-AKAP complex". Neuron 27 (1): 107–19. doi:10.1016/S0896-6273(00)00013-1. PMID 10939335.
• Indolfi C, Stabile E, Coppola C; et al. (2001). "Membrane-bound protein kinase A inhibits smooth muscle cell proliferation in vitro and in vivo by amplifying cAMP-protein kinase A signals". Circ. Res. 88 (3): 319–24. doi:10.1161/01.res.88.3.319. PMID 11179200.
• Dart C, Leyland ML (2001). "Targeting of an A kinase-anchoring protein, AKAP79, to an inwardly rectifying potassium channel, Kir2.1". J. Biol. Chem. 276 (23): 20499–505. doi:10.1074/jbc.M101425200. PMID 11287423.
• Xie G, Raufman JP (2001). "Association of protein kinase A with AKAP150 facilitates pepsinogen secretion from gastric chief cells". Am. J. Physiol. Gastrointest. Liver Physiol. 281 (4): G1051–8. PMID 11557526.