AP2M1

Adaptor-related protein complex 2, mu 1 subunit
PDB rendering based on 1bw8.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1H6E​
Identifiers
Symbols	AP2M1 ; AP50; CLAPM1; mu2
External IDs	OMIM: 601024 MGI: 1298405 HomoloGene: 3000 GeneCards: AP2M1 Gene
Gene ontology Molecular function • signal sequence binding • transporter activity • protein binding • lipid binding • clathrin adaptor activity • low-density lipoprotein particle receptor binding Cellular component • mitochondrion • lysosomal membrane • cytosol • plasma membrane • AP-2 adaptor complex • endocytic vesicle membrane • clathrin-coated endocytic vesicle membrane • extracellular exosome Biological process • intracellular protein transport • epidermal growth factor receptor signaling pathway • synaptic transmission • axon guidance • viral process • antigen processing and presentation of exogenous peptide antigen via MHC class II • negative regulation of epidermal growth factor receptor signaling pathway • neurotrophin TRK receptor signaling pathway • ephrin receptor signaling pathway • regulation of defense response to virus by virus • membrane organization • clathrin-mediated endocytosis • negative regulation of protein localization to plasma membrane Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1173	11773
Ensembl	ENSG00000161203	ENSMUSG00000022841
UniProt	Q96CW1	P84091
RefSeq (mRNA)	NM_001025205	NM_001302970
RefSeq (protein)	NP_001020376	NP_001289899
Location (UCSC)	Chr 3: 184.17 – 184.18 Mb	Chr 16: 20.54 – 20.54 Mb
PubMed search

AP-2 complex subunit mu is a protein that in humans is encoded by the AP2M1 gene.^[1]

Function

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene.^[2]

Interactions

AP2M1 has been shown to interact with CTLA-4^[3][4] and Alpha-1B adrenergic receptor.^[5]

References

1. Druck T, Gu Y, Prabhala G, Cannizzaro LA, Park SH, Huebner K, Keen JH (Nov 1995). "Chromosome localization of human genes for clathrin adaptor polypeptides AP2 beta and AP50 and the clathrin-binding protein, VCP". Genomics 30 (1): 94–7. doi:10.1006/geno.1995.0016. PMID 8595912.
2. "Entrez Gene: AP2M1 adaptor-related protein complex 2, mu 1 subunit".
3. Follows ER, McPheat JC, Minshull C, Moore NC, Pauptit RA, Rowsell S, Stacey CL, Stanway JJ, Taylor IW, Abbott WM (Oct 2001). "Study of the interaction of the medium chain mu 2 subunit of the clathrin-associated adapter protein complex 2 with cytotoxic T-lymphocyte antigen 4 and CD28". The Biochemical Journal 359 (Pt 2): 427–34. doi:10.1042/0264-6021:3590427. PMC 1222163. PMID 11583591.
4. Chuang E, Alegre ML, Duckett CS, Noel PJ, Vander Heiden MG, Thompson CB (Jul 1997). "Interaction of CTLA-4 with the clathrin-associated protein AP50 results in ligand-independent endocytosis that limits cell surface expression". Journal of Immunology 159 (1): 144–51. PMID 9200449.
5. Diviani D, Lattion AL, Abuin L, Staub O, Cotecchia S (May 2003). "The adaptor complex 2 directly interacts with the alpha 1b-adrenergic receptor and plays a role in receptor endocytosis". The Journal of Biological Chemistry 278 (21): 19331–40. doi:10.1074/jbc.M302110200. PMID 12644451.

Further reading

• Ohno H, Stewart J, Fournier MC, Bosshart H, Rhee I, Miyatake S, Saito T, Gallusser A, Kirchhausen T, Bonifacino JS (Sep 1995). "Interaction of tyrosine-based sorting signals with clathrin-associated proteins". Science 269 (5232): 1872–5. doi:10.1126/science.7569928. PMID 7569928.
• Liu Q, Feng Y, Forgac M (Dec 1994). "Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2". The Journal of Biological Chemistry 269 (50): 31592–7. PMID 7989329.
• Pauloin A, Thurieau C (Dec 1993). "The 50 kDa protein subunit of assembly polypeptide (AP) AP-2 adaptor from clathrin-coated vesicles is phosphorylated on threonine-156 by AP-1 and a soluble AP50 kinase which co-purifies with the assembly polypeptides". The Biochemical Journal. 296 ( Pt 2) (2): 409–15. PMC 1137711. PMID 8257432.
• Chang MP, Mallet WG, Mostov KE, Brodsky FM (May 1993). "Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits". The EMBO Journal 12 (5): 2169–80. PMC 413438. PMID 8491205.
• Wang X, Kilimann MW (Jan 1997). "Identification of two new mu-adaptin-related proteins, mu-ARP1 and mu-ARP2". FEBS Letters 402 (1): 57–61. doi:10.1016/S0014-5793(96)01500-1. PMID 9013859.
• Chuang E, Alegre ML, Duckett CS, Noel PJ, Vander Heiden MG, Thompson CB (Jul 1997). "Interaction of CTLA-4 with the clathrin-associated protein AP50 results in ligand-independent endocytosis that limits cell surface expression". Journal of Immunology 159 (1): 144–51. PMID 9200449.
• Zhang Y, Allison JP (Aug 1997). "Interaction of CTLA-4 with AP50, a clathrin-coated pit adaptor protein". Proceedings of the National Academy of Sciences of the United States of America 94 (17): 9273–8. doi:10.1073/pnas.94.17.9273. PMC 23153. PMID 9256472.
• Ohno H, Aguilar RC, Fournier MC, Hennecke S, Cosson P, Bonifacino JS (Nov 1997). "Interaction of endocytic signals from the HIV-1 envelope glycoprotein complex with members of the adaptor medium chain family". Virology 238 (2): 305–15. doi:10.1006/viro.1997.8839. PMID 9400603.
• Santini F, Marks MS, Keen JH (May 1998). "Endocytic clathrin-coated pit formation is independent of receptor internalization signal levels". Molecular Biology of the Cell 9 (5): 1177–94. doi:10.1091/mbc.9.5.1177. PMC 25339. PMID 9571248.
• Owen DJ, Evans PR (Nov 1998). "A structural explanation for the recognition of tyrosine-based endocytotic signals". Science 282 (5392): 1327–32. doi:10.1126/science.282.5392.1327. PMID 9812899.
• Berlioz-Torrent C, Shacklett BL, Erdtmann L, Delamarre L, Bouchaert I, Sonigo P, Dokhelar MC, Benarous R (Feb 1999). "Interactions of the cytoplasmic domains of human and simian retroviral transmembrane proteins with components of the clathrin adaptor complexes modulate intracellular and cell surface expression of envelope glycoproteins". Journal of Virology 73 (2): 1350–61. PMC 103959. PMID 9882340.
• Teuchert M, Berghöfer S, Klenk HD, Garten W (Dec 1999). "Recycling of furin from the plasma membrane. Functional importance of the cytoplasmic tail sorting signals and interaction with the AP-2 adaptor medium chain subunit". The Journal of Biological Chemistry 274 (51): 36781–9. doi:10.1074/jbc.274.51.36781. PMID 10593987.
• Umeda A, Meyerholz A, Ungewickell E (May 2000). "Identification of the universal cofactor (auxilin 2) in clathrin coat dissociation". European Journal of Cell Biology 79 (5): 336–42. doi:10.1078/S0171-9335(04)70037-0. PMID 10887964.
• Jullien-Flores V, Mahé Y, Mirey G, Leprince C, Meunier-Bisceuil B, Sorkin A, Camonis JH (Aug 2000). "RLIP76, an effector of the GTPase Ral, interacts with the AP2 complex: involvement of the Ral pathway in receptor endocytosis". Journal of Cell Science. 113 ( Pt 16) (16): 2837–44. PMID 10910768.
• Wyss S, Berlioz-Torrent C, Boge M, Blot G, Höning S, Benarous R, Thali M (Mar 2001). "The highly conserved C-terminal dileucine motif in the cytosolic domain of the human immunodeficiency virus type 1 envelope glycoprotein is critical for its association with the AP-1 clathrin adaptor [correction of adapter]". Journal of Virology 75 (6): 2982–92. doi:10.1128/JVI.75.6.2982-2992.2001. PMC 115924. PMID 11222723.
• Morris SM, Cooper JA (Feb 2001). "Disabled-2 colocalizes with the LDLR in clathrin-coated pits and interacts with AP-2". Traffic 2 (2): 111–23. doi:10.1034/j.1600-0854.2001.020206.x. PMID 11247302.
• Follows ER, McPheat JC, Minshull C, Moore NC, Pauptit RA, Rowsell S, Stacey CL, Stanway JJ, Taylor IW, Abbott WM (Oct 2001). "Study of the interaction of the medium chain mu 2 subunit of the clathrin-associated adapter protein complex 2 with cytotoxic T-lymphocyte antigen 4 and CD28". The Biochemical Journal 359 (Pt 2): 427–34. doi:10.1042/0264-6021:3590427. PMC 1222163. PMID 11583591.
• Friocourt G, Chafey P, Billuart P, Koulakoff A, Vinet MC, Schaar BT, McConnell SK, Francis F, Chelly J (Sep 2001). "Doublecortin interacts with mu subunits of clathrin adaptor complexes in the developing nervous system". Molecular and Cellular Neurosciences 18 (3): 307–19. doi:10.1006/mcne.2001.1022. PMID 11591131.