ARFGEF2

ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3L8N​, 3SWV​
Identifiers
Symbols	ARFGEF2 ; BIG2; PVNH2; dJ1164I10.1
External IDs	OMIM: 605371 MGI: 2139354 HomoloGene: 111880 GeneCards: ARFGEF2 Gene
Gene ontology Molecular function • guanyl-nucleotide exchange factor activity • ARF guanyl-nucleotide exchange factor activity • protein binding • myosin binding • protein kinase A regulatory subunit binding • GABA receptor binding Cellular component • Golgi membrane • trans-Golgi network • microtubule organizing center • cytosol • axonemal microtubule • membrane • cytoplasmic membrane-bounded vesicle • cell junction • cytoplasmic vesicle • asymmetric synapse • symmetric synapse • dendritic spine • perinuclear region of cytoplasm • recycling endosome Biological process • receptor recycling • exocytosis • Golgi to plasma membrane transport • endosome organization • endomembrane system organization • protein transport • regulation of ARF protein signal transduction • positive regulation of tumor necrosis factor production • intracellular signal transduction • positive regulation of GTPase activity Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10564	99371
Ensembl	ENSG00000124198	ENSMUSG00000074582
UniProt	Q9Y6D5	A2A5R2
RefSeq (mRNA)	NM_006420	NM_001085495
RefSeq (protein)	NP_006411	NP_001078964
Location (UCSC)	Chr 20: 48.92 – 49.04 Mb	Chr 2: 166.81 – 166.9 Mb
PubMed search

Brefeldin A-inhibited guanine nucleotide-exchange protein 2 is a protein that in humans is encoded by the ARFGEF2 gene.^[1][2]

Function

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.^[2]

Interactions

ARFGEF2 has been shown to interact with ARFGEF1,^[3] PRKAR1A^[4] and PRKAR2A.^[4]

References

1. Togawa A, Morinaga N, Ogasawara M, Moss J, Vaughan M (Apr 1999). "Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors". The Journal of Biological Chemistry 274 (18): 12308–15. doi:10.1074/jbc.274.18.12308. PMID 10212200.
2. "Entrez Gene: ARFGEF2 ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)".
3. Yamaji R, Adamik R, Takeda K, Togawa A, Pacheco-Rodriguez G, Ferrans VJ, Moss J, Vaughan M (Mar 2000). "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex". Proceedings of the National Academy of Sciences of the United States of America 97 (6): 2567–72. doi:10.1073/pnas.97.6.2567. PMC 15969. PMID 10716990.
4. Li H, Adamik R, Pacheco-Rodriguez G, Moss J, Vaughan M (Feb 2003). "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)". Proceedings of the National Academy of Sciences of the United States of America 100 (4): 1627–32. doi:10.1073/pnas.0337678100. PMC 149883. PMID 12571360.

Further reading

• Yamaji R, Adamik R, Takeda K, Togawa A, Pacheco-Rodriguez G, Ferrans VJ, Moss J, Vaughan M (Mar 2000). "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex". Proceedings of the National Academy of Sciences of the United States of America 97 (6): 2567–72. doi:10.1073/pnas.97.6.2567. PMC 15969. PMID 10716990.
• Kamei Y, Takeda Y, Teramoto K, Tsutsumi O, Taketani Y, Watanabe K (Oct 2000). "Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members". Genomics 69 (1): 113–9. doi:10.1006/geno.2000.6310. PMID 11013081.
• Pacheco-Rodriguez G, Moss J, Vaughan M (2002). "BIG1 and BIG2: brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors". Methods in Enzymology 345: 397–404. doi:10.1016/S0076-6879(02)45032-X. PMID 11665623.
• Shinotsuka C, Yoshida Y, Kawamoto K, Takatsu H, Nakayama K (Mar 2002). "Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation". The Journal of Biological Chemistry 277 (11): 9468–73. doi:10.1074/jbc.M112427200. PMID 11777925.
• Shinotsuka C, Waguri S, Wakasugi M, Uchiyama Y, Nakayama K (Jun 2002). "Dominant-negative mutant of BIG2, an ARF-guanine nucleotide exchange factor, specifically affects membrane trafficking from the trans-Golgi network through inhibiting membrane association of AP-1 and GGA coat proteins". Biochemical and Biophysical Research Communications 294 (2): 254–60. doi:10.1016/S0006-291X(02)00456-4. PMID 12051703.
• Li H, Adamik R, Pacheco-Rodriguez G, Moss J, Vaughan M (Feb 2003). "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)". Proceedings of the National Academy of Sciences of the United States of America 100 (4): 1627–32. doi:10.1073/pnas.0337678100. PMC 149883. PMID 12571360.
• Sheen VL, Ganesh VS, Topcu M, Sebire G, Bodell A, Hill RS, Grant PE, Shugart YY, Imitola J, Khoury SJ, Guerrini R, Walsh CA (Jan 2004). "Mutations in ARFGEF2 implicate vesicle trafficking in neural progenitor proliferation and migration in the human cerebral cortex". Nature Genetics 36 (1): 69–76. doi:10.1038/ng1276. PMID 14647276.
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (Jul 2004). "Functional proteomics mapping of a human signaling pathway". Genome Research 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Xu KF, Shen X, Li H, Pacheco-Rodriguez G, Moss J, Vaughan M (Feb 2005). "Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70". Proceedings of the National Academy of Sciences of the United States of America 102 (8): 2784–9. doi:10.1073/pnas.0409871102. PMC 549493. PMID 15705715.
• Lu J, Tiao G, Folkerth R, Hecht J, Walsh C, Sheen V (Jan 2006). "Overlapping expression of ARFGEF2 and Filamin A in the neuroependymal lining of the lateral ventricles: insights into the cause of periventricular heterotopia". The Journal of Comparative Neurology 494 (3): 476–84. doi:10.1002/cne.20806. PMID 16320251.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Islam A, Shen X, Hiroi T, Moss J, Vaughan M, Levine SJ (Mar 2007). "The brefeldin A-inhibited guanine nucleotide-exchange protein, BIG2, regulates the constitutive release of TNFR1 exosome-like vesicles". The Journal of Biological Chemistry 282 (13): 9591–9. doi:10.1074/jbc.M607122200. PMID 17276987.
• Kuroda F, Moss J, Vaughan M (Feb 2007). "Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1gamma". Proceedings of the National Academy of Sciences of the United States of America 104 (9): 3201–6. doi:10.1073/pnas.0611696104. PMC 1805514. PMID 17360629.