ARHGEF2

Rho/Rac guanine nucleotide exchange factor (GEF) 2
Identifiers
Symbols	ARHGEF2 ; GEF; GEF-H1; GEFH1; LFP40; P40
External IDs	OMIM: 607560 MGI: 103264 HomoloGene: 3468 GeneCards: ARHGEF2 Gene
Gene ontology Molecular function • Rho guanyl-nucleotide exchange factor activity • protein binding • microtubule binding • transcription factor binding • zinc ion binding • Rho GTPase binding • Rac guanyl-nucleotide exchange factor activity • Rac GTPase binding Cellular component • cytoplasm • Golgi apparatus • spindle • cytosol • cytoskeleton • microtubule • bicellular tight junction • focal adhesion • cytoplasmic membrane-bounded vesicle • vesicle • ruffle membrane • neuronal cell body • dendritic shaft • protein complex • neuronal postsynaptic density Biological process • establishment of mitotic spindle orientation • cell morphogenesis • intracellular protein transport • actin filament organization • negative regulation of microtubule depolymerization • mitotic nuclear division • small GTPase mediated signal transduction • positive regulation of interleukin-6 production • positive regulation of tumor necrosis factor production • regulation of Rho protein signal transduction • regulation of cell proliferation • positive regulation of apoptotic process • positive regulation of GTPase activity • innate immune response • positive regulation of transcription from RNA polymerase II promoter • neurotrophin TRK receptor signaling pathway • positive regulation of peptidyl-tyrosine phosphorylation • negative regulation of neurogenesis • regulation of small GTPase mediated signal transduction • positive regulation of NF-kappaB transcription factor activity • cell division • negative regulation of necroptotic process • cellular response to muramyl dipeptide • cellular response to tumor necrosis factor • cellular hyperosmotic response • apoptotic signaling pathway • negative regulation of extrinsic apoptotic signaling pathway via death domain receptors • negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9181	16800
Ensembl	ENSG00000116584	ENSMUSG00000028059
UniProt	Q92974	Q60875
RefSeq (mRNA)	NM_001162383	NM_001198911
RefSeq (protein)	NP_001155855	NP_001185840
Location (UCSC)	Chr 1: 155.95 – 156.01 Mb	Chr 3: 88.61 – 88.65 Mb
PubMed search

Rho guanine nucleotide exchange factor 2 is a protein that in humans is encoded by the ARHGEF2 gene.^[1][2][3]

Function

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein-coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals.^[3]

Interactions

ARHGEF2 has been shown to interact with PAK1.^[4]

References

1. Ren Y, Li R, Zheng Y, Busch H (Feb 1999). "Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases". J Biol Chem 273 (52): 34954–60. doi:10.1074/jbc.273.52.34954. PMID 9857026.
2. Ishikawa K, Nagase T, Suyama M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Dec 1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.
3. "Entrez Gene: ARHGEF2 rho/rac guanine nucleotide exchange factor (GEF) 2".
4. Zenke FT, Krendel M, DerMardirossian C, King CC, Bohl BP, Bokoch GM (Apr 2004). "p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor". J. Biol. Chem. 279 (18): 18392–400. doi:10.1074/jbc.M400084200. PMID 14970201.

Further reading

• Ehrhardt A, Ehrhardt GR, Guo X, Schrader JW (2002). "Ras and relatives--job sharing and networking keep an old family together". Exp. Hematol. 30 (10): 1089–106. doi:10.1016/S0301-472X(02)00904-9. PMID 12384139.
• Reddy AB, Chatterjee A, Rothblum LI, Black A, Busch H (1989). "Isolation and characterization of complementary DNA to proliferating cell nucleolar antigen P40". Cancer Res. 49 (7): 1763–7. PMID 2466560.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Gao Y, Xing J, Streuli M, Leto TL, Zheng Y (2002). "Trp(56) of rac1 specifies interaction with a subset of guanine nucleotide exchange factors". J. Biol. Chem. 276 (50): 47530–41. doi:10.1074/jbc.M108865200. PMID 11595749.
• Krendel M, Zenke FT, Bokoch GM (2002). "Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton". Nat. Cell Biol. 4 (4): 294–301. doi:10.1038/ncb773. PMID 11912491.
• Brajenovic M, Joberty G, Küster B, Bouwmeester T, Drewes G (2004). "Comprehensive proteomic analysis of human Par protein complexes reveals an interconnected protein network". J. Biol. Chem. 279 (13): 12804–11. doi:10.1074/jbc.M312171200. PMID 14676191.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216.
• Zenke FT, Krendel M, DerMardirossian C, King CC, Bohl BP, Bokoch GM (2004). "p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor". J. Biol. Chem. 279 (18): 18392–400. doi:10.1074/jbc.M400084200. PMID 14970201.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Callow MG, Zozulya S, Gishizky ML, Jallal B, Smeal T (2005). "PAK4 mediates morphological changes through the regulation of GEF-H1". J. Cell. Sci. 118 (Pt 9): 1861–72. doi:10.1242/jcs.02313. PMID 15827085.
• Aijaz S, D'Atri F, Citi S, Balda MS, Matter K (2005). "Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition". Dev. Cell 8 (5): 777–86. doi:10.1016/j.devcel.2005.03.003. PMID 15866167.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.