ATP5F1

ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
Identifiers
Symbols	ATP5F1 ; PIG47
External IDs	OMIM: 603270 MGI: 1100495 HomoloGene: 1275 GeneCards: ATP5F1 Gene
Gene ontology Molecular function • protein binding • ATPase activity • transmembrane transporter activity • proton-transporting ATP synthase activity, rotational mechanism Cellular component • mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) • nucleus • nucleoplasm • mitochondrion • mitochondrial inner membrane • mitochondrial proton-transporting ATP synthase complex • mitochondrial matrix • membrane • myelin sheath • extracellular exosome Biological process • ATP synthesis coupled proton transport • substantia nigra development • respiratory electron transport chain • mitochondrial ATP synthesis coupled proton transport • cellular metabolic process • small molecule metabolic process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	515	11950
Ensembl	ENSG00000116459	ENSMUSG00000000563
UniProt	P24539	Q9CQQ7
RefSeq (mRNA)	NM_001688	NM_001304719
RefSeq (protein)	NP_001679	NP_001291648
Location (UCSC)	Chr 1: 111.45 – 111.46 Mb	Chr 3: 105.94 – 105.96 Mb
PubMed search

ATP-synt_B
atp synthase b subunit dimerization domain
Identifiers
Symbol	ATP-synt_B
Pfam	PF00430
Pfam clan	CL0255
InterPro	IPR002146
SCOP	1b9u
SUPERFAMILY	1b9u
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

ATP synthase subunit b, mitochondrial is an enzyme that in humans is encoded by the ATP5F1 gene.^[1][2]

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.^[2]

The b subunits are part of the peripheral stalk that links the F1 and FO complexes together, and which acts as a stator to prevent certain subunits from rotating with the central rotary element. The peripheral stalk differs in subunit composition between mitochondrial, chloroplast and bacterial F-ATPases. In bacterial and chloroplast F-ATPases, the peripheral stalk is composed of one copy of the delta subunit (homologous to OSCP in mitochondria), and two copies of subunit b in bacteria, or one copy each of subunits b and b' in chloroplasts and photosynthetic bacteria.^[3]

References

1. Higuti T, Tsurumi C, Osaka F, Kawamura Y, Tsujita H, Yoshihara Y, Tani I, Tanaka K, Ichihara A (Sep 1991). "Molecular cloning of cDNA for the import precursor of human subunit B of H(+)-ATP synthase in mitochondria". Biochem Biophys Res Commun 178 (3): 1014–20. doi:10.1016/0006-291X(91)90993-H. PMID 1831354.
2. "Entrez Gene: ATP5F1 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1".
3. Carbajo RJ, Kellas FA, Runswick MJ, Montgomery MG, Walker JE, Neuhaus D (August 2005). "Structure of the F1-binding domain of the stator of bovine F1Fo-ATPase and how it binds an alpha-subunit". J. Mol. Biol. 351 (4): 824–38. doi:10.1016/j.jmb.2005.06.012. PMID 16045926.

Further reading

• Gay NJ, Walker JE (1986). "Two genes encoding the bovine mitochondrial ATP synthase proteolipid specify precursors with different import sequences and are expressed in a tissue-specific manner". EMBO J. 4 (13A): 3519–24. PMC 554691. PMID 2868890.
• Farrell LB, Nagley P (1987). "Human liver cDNA clones encoding proteolipid subunit 9 of the mitochondrial ATPase complex". Biochem. Biophys. Res. Commun. 144 (3): 1257–64. doi:10.1016/0006-291X(87)91446-X. PMID 2883974.
• Houstĕk J, Andersson U, Tvrdík P, et al. (1995). "The expression of subunit c correlates with and thus may limit the biosynthesis of the mitochondrial F0F1-ATPase in brown adipose tissue". J. Biol. Chem. 270 (13): 7689–94. doi:10.1074/jbc.270.13.7689. PMID 7706317.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Elston T, Wang H, Oster G (1998). "Energy transduction in ATP synthase". Nature 391 (6666): 510–3. doi:10.1038/35185. PMID 9461222.
• Wang H, Oster G (1998). "Energy transduction in the F1 motor of ATP synthase". Nature 396 (6708): 279–82. doi:10.1038/24409. PMID 9834036.
• Jia L, Young MF, Powell J, et al. (2002). "Gene expression profile of human bone marrow stromal cells: high-throughput expressed sequence tag sequencing analysis". Genomics 79 (1): 7–17. doi:10.1006/geno.2001.6683. PMID 11827452.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Cross RL (2004). "Molecular motors: turning the ATP motor". Nature 427 (6973): 407–8. doi:10.1038/427407b. PMID 14749816.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature 441 (7091): 315–21. doi:10.1038/nature04727. PMID 16710414.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

This article incorporates text from the public domain Pfam and InterPro IPR002146