Actinobacterial phage holin family

The Actinobacterial Phage Holin (APH) Family (TC# 1.E.57) is a fairly large family of proteins between 105 and 180 amino acyl residues in length, typically exhibiting a single transmembrane segment (TMS) near the N-terminus. A representative list of proteins belonging to the APH family can be found in the Transporter Classification Database.[1]

One of the archetype proteins in this family is the Gp5 holin of mycobacteriophage Ms6. Mycobacteriophage Ms6 is a double-stranded DNA (dsDNA) bacteriophage which, in addition to the predicted endolysin (LysA)-holin (Gp4) lysis system, encodes three additional proteins within its lysis module: Gp1, LysB, and Gp5.[2]

Ms6 Gp4 (TC# 1.E.18.1.2) was previously described as a class II holin-like protein. A second putative holin gene (gp5) encoding a protein (Gp5) with a predicted single N-terminal TMS was identified at the end of the Ms6 lytic operon. Neither the putative class II holin nor the single TMS polypeptide could trigger lysis in pairwise combinations with the endolysin LysA in Escherichia coli. However, further studies have shown that Ms6's Gp4 and Gp5 interact with each other. This suggests that in Ms6 infection, the correct and programmed timing of lysis is achieved by the combined action of Gp4 and Gp5.[2]

See also

Further reading

References

  1. "1.E.57. The Actinobacterial Phage Holin (APH) Family". TCDB. Retrieved 2016-03-29.
  2. 1 2 Catalão, Maria João; Gil, Filipa; Moniz-Pereira, José; Pimentel, Madalena (2011-06-01). "Functional analysis of the holin-like proteins of mycobacteriophage Ms6". Journal of Bacteriology 193 (11): 2793–2803. doi:10.1128/JB.01519-10. ISSN 1098-5530. PMC 3133122. PMID 21441511.

As of this edit, this article uses content from "1.E.57. The Actinobacterial Phage Holin (APH) Family", which is licensed in a way that permits reuse under the Creative Commons Attribution-ShareAlike 3.0 Unported License, but not under the GFDL. All relevant terms must be followed.

This article is issued from Wikipedia - version of the Thursday, March 31, 2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.