Acyl-homoserine-lactone synthase

Acyl-homoserine-lactone synthase
Identifiers
EC number 2.3.1.184
CAS number 176023-66-8
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Acyl-homoserine-lactone synthase (EC 2.3.1.184) is an enzyme with systematic name acyl-(acyl-carrier protein):S-adenosyl-L-methionine acyltranserase (lactone-forming, methylthioadenosine-releasing).[1][2][3][4][5][6][7][8][9] This enzyme catalyses the following chemical reaction

acyl-[acyl-carrier protein] + S-adenosyl-L-methionine \rightleftharpoons [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-acyl-L-homoserine lactone

Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing.

Alternate names

acyl-homoserine lactone synthase, acyl homoserine lactone synthase, acyl-homoserinelactone synthase, acylhomoserine lactone synthase, AHL synthase, AHS, AHSL synthase, AhyI, AinS, AinS protein, autoinducer synthase, autoinducer synthesis protein rhlI, EsaI, ExpISCC1, ExpISCC3065, LasI, LasR, LuxI, LuxI protein, LuxM, N-acyl homoserine lactone synthase, RhlI, YspI, acyl-[acyl carrier protein]:S-adenosyl-L-methionine acyltranserase (lactone-forming, methylthioadenosine-releasing)

References

  1. ↑ Schaefer, A.L., Val, D.L., Hanzelka, B.L., Cronan, J.E., Jr. and Greenberg, E.P. (1996). "Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein". Proc. Natl. Acad. Sci. USA 93 (18): 9505–9509. doi:10.1073/pnas.93.18.9505. PMID 8790360.
  2. ↑ Watson, W.T., Murphy, F.V., 4th, Gould, T.A., Jambeck, P., Val, D.L., Cronan, J.E., Jr., Beck von Bodman, S. and Churchill, M.E. (2001). "Crystallization and rhenium MAD phasing of the acyl-homoserinelactone synthase EsaI". Acta Crystallogr. D Biol. Crystallogr. 57 (Pt 12): 1945–1949. doi:10.1107/s0907444901014512. PMID 11717525.
  3. ↑ Chakrabarti, S. and Sowdhamini, R. (2003). "Functional sites and evolutionary connections of acylhomoserine lactone synthases". Protein Eng. 16 (4): 271–278. doi:10.1093/proeng/gzg031. PMID 12736370.
  4. ↑ Hanzelka, B.L., Parsek, M.R., Val, D.L., Dunlap, P.V., Cronan, J.E., Jr. and Greenberg, E.P. (1999). "Acylhomoserine lactone synthase activity of the Vibrio fischeri AinS protein". J. Bacteriol. 181 (18): 5766–5770. PMID 10482519.
  5. ↑ Parsek, M.R., Val, D.L., Hanzelka, B.L., Cronan, J.E., Jr. and Greenberg, E.P. (1999). "Acyl homoserine-lactone quorum-sensing signal generation". Proc. Natl. Acad. Sci. USA 96 (8): 4360–4365. doi:10.1073/pnas.96.8.4360. PMID 10200267.
  6. ↑ Ulrich, R.L. (2004). "Quorum quenching: enzymatic disruption of N-acylhomoserine lactone-mediated bacterial communication in Burkholderia thailandensis". Appl. Environ. Microbiol. 70: 6173–6180. doi:10.1128/AEM.70.10.6173-6180.2004. PMID 15466564.
  7. ↑ Gould, T.A., Schweizer, H.P. and Churchill, M.E. (2004). "Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI". Mol. Microbiol. 53 (4): 1135–1146. doi:10.1111/j.1365-2958.2004.04211.x. PMID 15306017.
  8. ↑ Raychaudhuri, A., Jerga, A. and Tipton, P.A. (2005). "Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa". Biochemistry 44 (8): 2974–2981. doi:10.1021/bi048005m. PMID 15723540.
  9. ↑ Gould, T.A., Herman, J., Krank, J., Murphy, R.C. and Churchill, M.E. (2006). "Specificity of acyl-homoserine lactone synthases examined by mass spectrometry". J. Bacteriol. 188 (2): 773–783. doi:10.1128/JB.188.2.773-783.2006. PMID 16385066.

External links

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