Aliphatic (R)-hydroxynitrile lyase

Aliphatic (R)-hydroxynitrile lyase (EC 4.1.2.46, (R)-HNL, (R)-oxynitrilase, (R)-hydroxynitrile lyase, LuHNL) is an enzyme with systematic name (2R)-2-hydroxy-2-methylbutanenitrile butan-2-one-lyase (cyanide forming).^[1]^[2]^[3]^[4]^[5]^[6] This enzyme catalyses the following chemical reaction

(2R)-2-hydroxy-2-methylbutanenitrile

\rightleftharpoons

cyanide + butan-2-one

The enzyme contains Zn²⁺.

References

↑ Trummler, K., Roos, J., Schwaneberg, U., Effenberger, F., Förster, S., Pfizenmaier, K. and Wajant, H. (1998). "Expression of the Zn²⁺-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris— utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis". Plant Sci. 139: 19–27. doi:10.1016/s0168-9452(98)00173-3.
↑ Trummler, K. and Wajant, H. (1997). "Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases". J. Biol. Chem. 272 (8): 4770–4774. doi:10.1074/jbc.272.8.4770. PMID 9030531.
↑ Albrecht, J., Jansen, I.and Kula, M.R. (1993). "Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range". Biotechnol. Appl. Biochem. 17: 191–203.
↑ Xu, L.-L., Singh, B.K. and Conn, E.E. (1988). "Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum". Arch. Biochem. Biophys. 263: 256–263. doi:10.1016/0003-9861(88)90634-0. PMID 3377504.
↑ Cabirol, F.L., Tan, P.L., Tay, B., Cheng, S., Hanefeld, U. and Sheldon, R.A. (2008). "Linum usitatissimum hydroxynitrile lyase cross-linked enzyme aggregates: a recyclable enantioselective catalyst". Adv. Synth. Catal. 350: 2329–2338. doi:10.1002/adsc.200800309.
↑ Breithaupt, H., Pohl, M., BÃ¶nigk, W., Heim, P., Schimz, K.-L. and Kula, M.-R. (1999). "Cloning and expression of (R)-hydroxynitrile lyase from Linum usitatissimum (flax)". J. Mol. Catal. B 6: 315–332. doi:10.1016/s1381-1177(98)00109-x.

External links

Aliphatic (R)-hydroxynitrile lyase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-carbon lyases (EC 4.1)

4.1.1: Carboxy-lyases	Acetoacetate decarboxylase Adenosylmethionine decarboxylase Aromatic L-amino acid decarboxylase Glutamate decarboxylase Histidine decarboxylase Lysine decarboxylase Malonyl-CoA decarboxylase Ornithine decarboxylase Oxaloacetate decarboxylase Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxylase Phosphoribosylaminoimidazole carboxylase Pyrophosphomevalonate decarboxylase Pyruvate decarboxylase RuBisCO Uridine monophosphate synthetase/Orotidine 5'-phosphate decarboxylase Uroporphyrinogen III decarboxylase

4.1.2: Aldehyde-lyases	Fructose-bisphosphate aldolase Aldolase A Aldolase B Aldolase C 2-hydroxyphytanoyl-CoA lyase Threonine aldolase

4.1.3: Oxo-acid-lyases	Isocitrate lyase 3-hydroxy-3-methylglutaryl-CoA lyase

4.1.99: Other	Tryptophanase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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