Amido black 10B

Amido black 10B
Space-filling model of the amido black 10B molecule
Names
IUPAC name
4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt
Other names
Amidoschwarz
Naphthol blue black
Acid Black 1
Acidal Black 10B
Acidal Navy Blue 3BR
Naphthalene Black 10B (see talk page)
Buffalo Black NBR
C.I. 20470
Identifiers
1064-48-8 YesY
ChEBI CHEBI:86230 N
ChemSpider 10660833 YesY
Jmol interactive 3D Image
PubChem 9566044
Properties
C22H14N6Na2O9S2
Molar mass 616.49 g·mol−1
Appearance Dark red to black
~ 30 g/l at 20 °C (68 °F)
Hazards
R-phrases R20, R21, R33
S-phrases S28A, S37, S45
NFPA 704
Flammability code 0: Will not burn. E.g., water Health code 2: Intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury. E.g., chloroform Reactivity code 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g., liquid nitrogen Special hazards (white): no codeNFPA 704 four-colored diamond
0
2
0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N verify (what is YesYN ?)
Infobox references

Amido black 10B is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

External links

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