BTAF1

BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
Identifiers
Symbols	BTAF1 ; MOT1; TAF(II)170; TAF172; TAFII170
External IDs	OMIM: 605191 MGI: 2147538 HomoloGene: 31978 GeneCards: BTAF1 Gene
Gene ontology Molecular function • DNA binding • transcription factor activity, sequence-specific DNA binding • helicase activity • ATP binding Cellular component • nucleus Biological process • metabolic process • negative regulation of chromatin binding • negative regulation of transcription, DNA-templated Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9044	107182
Ensembl	ENSG00000095564	ENSMUSG00000040565
UniProt	O14981	E9QAE3
RefSeq (mRNA)	NM_003972	NM_001080706
RefSeq (protein)	NP_003963	NP_001074175
Location (UCSC)	Chr 10: 91.92 – 92.03 Mb	Chr 19: 36.93 – 37.01 Mb
PubMed search

TATA-binding protein-associated factor 172 is a protein that in humans is encoded by the BTAF1 gene.^[1][2][3]

Function

Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.[supplied by OMIM]^[3]

Interactions

BTAF1 has been shown to interact with TATA binding protein.^[2][4]

References

1. van der Knaap JA, Borst JW, van der Vliet PC, Gentz R, Timmers HT (Dec 1997). "Cloning of the cDNA for the TATA-binding protein-associated factorII170 subunit of transcription factor B-TFIID reveals homology to global transcription regulators in yeast and Drosophila". Proc Natl Acad Sci U S A 94 (22): 11827–32. doi:10.1073/pnas.94.22.11827. PMC 23617. PMID 9342322.
2. Chicca JJ, Auble DT, Pugh BF (Mar 1998). "Cloning and Biochemical Characterization of TAF-172, a Human Homolog of Yeast Mot1". Mol Cell Biol 18 (3): 1701–10. PMC 108885. PMID 9488487.
3. "Entrez Gene: BTAF1 BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)".
4. Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD (May 1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605–10. doi:10.1073/pnas.94.11.5605. PMC 20825. PMID 9159119.

Further reading

• Zhou Q, Sharp PA (1995). "Novel mechanism and factor for regulation by HIV-1 Tat". EMBO J. 14 (2): 321–8. PMC 398086. PMID 7835343.
• Parada CA, Yoon JB, Roeder RG (1995). "A novel LBP-1-mediated restriction of HIV-1 transcription at the level of elongation in vitro". J. Biol. Chem. 270 (5): 2274–83. doi:10.1074/jbc.270.5.2274. PMID 7836461.
• Ou SH, Garcia-Martínez LF, Paulssen EJ, Gaynor RB (1994). "Role of flanking E box motifs in human immunodeficiency virus type 1 TATA element function". J. Virol. 68 (11): 7188–99. PMC 237158. PMID 7933101.
• Kashanchi F, Piras G, Radonovich MF, Duvall JF, Fattaey A, Chiang CM, Roeder RG, Brady JN (1994). "Direct interaction of human TFIID with the HIV-1 transactivator tat". Nature 367 (6460): 295–9. doi:10.1038/367295a0. PMID 8121496.
• Wang Z, Morris GF, Rice AP, Xiong W, Morris CB (1996). "Wild-type and transactivation-defective mutants of human immunodeficiency virus type 1 Tat protein bind human TATA-binding protein in vitro". J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 12 (2): 128–38. doi:10.1097/00042560-199606010-00005. PMID 8680883.
• Pendergrast PS, Morrison D, Tansey WP, Hernandez N (1996). "Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly". J. Virol. 70 (8): 5025–34. PMC 190456. PMID 8764009.
• Kashanchi F, Khleif SN, Duvall JF, Sadaie MR, Radonovich MF, Cho M, Martin MA, Chen SY, Weinmann R, Brady JN (1996). "Interaction of human immunodeficiency virus type 1 Tat with a unique site of TFIID inhibits negative cofactor Dr1 and stabilizes the TFIID-TFIIA complex". J. Virol. 70 (8): 5503–10. PMC 190508. PMID 8764062.
• Zhou Q, Sharp PA (1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science 274 (5287): 605–10. doi:10.1126/science.274.5287.605. PMID 8849451.
• García-Martínez LF, Ivanov D, Gaynor RB (1997). "Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes". J. Biol. Chem. 272 (11): 6951–8. doi:10.1074/jbc.272.11.6951. PMID 9054383.
• Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD (1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605–10. doi:10.1073/pnas.94.11.5605. PMC 20825. PMID 9159119.
• Van Der Knaap JA, Van Den Boom V, Kuipers J, Van Eijk MJ, Van Der Vliet PC, Timmers HT (2000). "The gene for human TATA-binding-protein-associated factor (TAFII) 170: structure, promoter and chromosomal localization". Biochem. J. 345 Pt 3 (Pt 3): 521–7. doi:10.1042/0264-6021:3450521. PMC 1220786. PMID 10642510.
• Wadhwa R, Yaguchi T, Hasan MK, Taira K, Kaul SC (2003). "Mortalin-MPD (mevalonate pyrophosphate decarboxylase) interactions and their role in control of cellular proliferation". Biochem. Biophys. Res. Commun. 302 (4): 735–42. doi:10.1016/S0006-291X(03)00226-2. PMID 12646231.
• Pereira LA, Klejman MP, Ruhlmann C, Kavelaars F, Oulad-Abdelghani M, Timmers HT, Schultz P (2004). "Molecular architecture of the basal transcription factor B-TFIID". J. Biol. Chem. 279 (21): 21802–7. doi:10.1074/jbc.M313519200. PMID 14988402.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Klejman MP, Pereira LA, van Zeeburg HJ, Gilfillan S, Meisterernst M, Timmers HT (2004). "NC2α Interacts with BTAF1 and Stimulates Its ATP-Dependent Association with TATA-Binding Protein". Mol. Cell. Biol. 24 (22): 10072–82. doi:10.1128/MCB.24.22.10072-10082.2004. PMC 525489. PMID 15509807.
• Grob P, Cruse MJ, Inouye C, Peris M, Penczek PA, Tjian R, Nogales E (2006). "Cryo-electron microscopy studies of human TFIID: conformational breathing in the integration of gene regulatory cues". Structure 14 (3): 511–20. doi:10.1016/j.str.2005.11.020. PMID 16531235.