Bisbenzimide

Bisbenzimide
Names
IUPAC name
2’-(4-Ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-1H,3’H-2,5’-bibenzimidazole
Other names
Hoechst 33342
Identifiers
23491-52-3
ChemSpider 1420
Jmol interactive 3D Image
PubChem 1464
Properties
C27H28N6O
Molar mass 452.56 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Bisbenzimide is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several bisbenzimides are used and are collectively called Hoechst stains.

Nuclei of Platynereis dumerilii larvae stained with Hoechst 33342.

Application

Bisbenzimides tend to bind to A-T rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.

References

This article is issued from Wikipedia - version of the Tuesday, December 15, 2015. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.