Buehler test
The Buehler test is an in vivo test to screen for substances that cause human skin sensitisation (i.e. allergens). It was first proposed by Edwin Vernon Buehler in 1965[1] and further explained in 1980.[2]
It is a non-adjuvant test. In the test, Guinea pigs are exposed to a high dose of the substance. They are then given a challenge dose, which is the highest dose that does not cause irritation.[3]
The test has been largely superseded by the murine local lymph node assay.
See also: guinea pig maximisation test
References
- ↑ Buehler E.V. (1965). Delayed contact hypersensitivity in the guinea pig. Archives of Dermatology, 91, 171
- ↑ Ritz, H. L.; Buehler, E. V. (1980). "Procedure for conducting the guinea pig assay". In Drill, V. A. and; Lazar, P. Current Concepts in Dermatology. New York: Academic Press. pp. 25–40.
- ↑ OECD (1992-07-17). "Test No. 406: Skin Sensitisation". OECD Guidelines for the Testing of Chemicals, Section 4. OECD Publishing. doi:10.1787/9789264070660-en. Retrieved 2012-02-17.
This article is issued from Wikipedia - version of the Monday, May 04, 2015. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.