C10orf118

Coiled-coil domain containing 186
Identifiers
Symbols CCDC186 ; C10orf118
External IDs MGI: 2445022 HomoloGene: 9963 GeneCards: CCDC186 Gene
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 55088 213993
Ensembl ENSG00000165813 ENSMUSG00000035173
UniProt Q7Z3E2 Q8C9S4
RefSeq (mRNA) NM_018017 NM_170757
RefSeq (protein) NP_060487 NP_739563
Location (UCSC) Chr 10:
114.12 – 114.17 Mb		 Chr 19:
56.79 – 56.82 Mb
PubMed search
Cartoon depiction of C10orf118 created with the Illustrator for Biology Sequences.[1]

C10orf118 is a protein that in humans is encoded by the C10orf118 gene [2] The C10orf118 (CCDC186) gene is also known as the CTCL-tumor associated antigen with accession number NM_018017.[3]

Gene

Location

C10orf118 has the chromosome location of 10q25.3 and is 53,750 bases in size oriented on the minus strand. PSORTII Protein k-NN Prediction indicated that C10orf118 is 65.2% nuclear, 17.4% cytosolic, 8.7% mitochondrial, 4.3% vesicles of secretory system, and 4.3% endoplasmic reticulum.[4]

Expression

Analysis of gene expression in humans and other species indicates C10orf118 (CCDC186) is ubiquitously expressed in all tissue types at varying developmental stages. An EST profile from NCBI displayed the greatest expression in bone marrow, kidneys, and the prostate cell lines. Breakdown by health state indicates high expression of C10orf118 in bladder carcinoma and prostate cancer.[5]

Homology

C10orf118 is conserved in many species. Orthologous sequences of C10orf118 were not found to be in bacteria, archea, protist, or plants. C10orf118 has no human paralogs.

Protein

General Properties

The protein of CCDC186 (NP_060487) is 898 amino acids in length. The predicted molecular weight is 103.7kdal and the isoelectric point is predicted to be 5.92.[6]

Composition

A serine rich region is observed in amino acids 710-747. A compositional analysis revealed that C10orf118 is Proline (1.1%) poor and Glutamic acid (14.1%) and Lysine (12.0%) rich.[6]

C10orf118 Phylogenetic Tree of orthologs.

Conservation

The date of divergence for the orthologous sequences highly correlates with the sequences similarity in that the percent identity decreases as you go back in time. Closely related orthologs include mammals and birds and moderately related orthologs include other vertebrates such as fish, reptiles, and amphibians. Distantly related orthologous sequences were primarily observed in invertebrates.[6][7]

Post Translational Modification

C10orf118 is predicted to undergo multiple posttranslational modifications including predicted O-beta-GlcNAc attachment, phosphorylation, a nuclear export signal, glycation of lysines, GlcNAc O-glycosylation, N-glycosylation, and NetCorona sites.[8]

Interacting Proteins

C10orf118 protein was found to interact with proteins PLEKAH5, Exra, GAMMAHV.ORF23, and SMAD3.[9][10]

Protein Model[11]

References

  1. ↑ http://ibs.biocuckoo.org/online.php
  2. ↑ Oduru, S; Campbell, JL; Karri, S; Hendry, WJ; Khan, SA; Williams, SC (Jul 2003). "Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs". BMC Genomics 4: 22. doi:10.1186/1471-2164-4-22. PMC 161800. PMID 12783626.
  3. ↑ "Entrez Gene: C10orf118 chromosome 10 open reading frame 118".
  4. ↑ "PSORT II Prediction". psort.hgc.jp. Retrieved 2016-04-24.
  5. ↑ "Home - UniGene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2016-04-24.
  6. 1 2 3 "SDSC Biology Workbench". seqtool.sdsc.edu. Retrieved 2016-04-24.
  7. ↑ "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2016-04-24.
  8. ↑ "Welcome to CBS". www.cbs.dtu.dk. Retrieved 2016-04-24.
  9. ↑ "* in Literature citations". www.uniprot.org. Retrieved 2016-04-24.
  10. ↑ http://www.uniprot.org/uniprot/Q7Z3E2
  11. ↑ "PHYRE2". www.sbg.bio.ic.ac.uk/phyre2. Retrieved 2016-02-05.

Further reading


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