CHFR

Checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
PDB rendering based on 1lgp.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1LGP​, 1LGQ​, 2XOC​, 2XOY​, 2XOZ​, 2XP0​
Identifiers
Symbols	CHFR ; RNF116; RNF196
External IDs	OMIM: 605209 MGI: 2444898 HomoloGene: 10078 GeneCards: CHFR Gene
Gene ontology Molecular function • nucleotide binding • ubiquitin-protein transferase activity • protein binding • zinc ion binding • ligase activity • ubiquitin protein ligase activity Cellular component • nucleus • PML body Biological process • protein polyubiquitination • ubiquitin-dependent protein catabolic process • mitotic nuclear division • mitotic cell cycle checkpoint • modification-dependent protein catabolic process • positive regulation of protein ubiquitination • protein destabilization • positive regulation of proteasomal ubiquitin-dependent protein catabolic process • cell division Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	55743	231600
Ensembl	ENSG00000072609	ENSMUSG00000014668
UniProt	Q96EP1	Q810L3
RefSeq (mRNA)	NM_001161344	NM_001289577
RefSeq (protein)	NP_001154816	NP_001276506
Location (UCSC)	Chr 12: 132.82 – 132.96 Mb	Chr 5: 110.14 – 110.17 Mb
PubMed search

E3 ubiquitin-protein ligase CHFR is an enzyme that in humans is encoded by the CHFR gene.^[1][2][3]

References

1. Scolnick DM, Halazonetis TD (Aug 2000). "Chfr defines a mitotic stress checkpoint that delays entry into metaphase". Nature 406 (6794): 430–5. doi:10.1038/35019108. PMID 10935642.
2. Kang D, Chen J, Wong J, Fang G (Jan 2002). "The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition". J Cell Biol 156 (2): 249–59. doi:10.1083/jcb.200108016. PMC 2199220. PMID 11807090.
3. "Entrez Gene: CHFR checkpoint with forkhead and ring finger domains".

Further reading

• Chaturvedi P, Sudakin V, Bobiak ML, et al. (2002). "Chfr regulates a mitotic stress pathway through its RING-finger domain with ubiquitin ligase activity". Cancer Res. 62 (6): 1797–801. PMID 11912157.
• Mizuno K, Osada H, Konishi H, et al. (2002). "Aberrant hypermethylation of the CHFR prophase checkpoint gene in human lung cancers". Oncogene 21 (15): 2328–33. doi:10.1038/sj.onc.1205402. PMID 11948416.
• Stavridi ES, Huyen Y, Loreto IR, et al. (2002). "Crystal structure of the FHA domain of the Chfr mitotic checkpoint protein and its complex with tungstate". Structure 10 (7): 891–9. doi:10.1016/S0969-2126(02)00776-1. PMID 12121644.
• Shibata Y, Haruki N, Kuwabara Y, et al. (2002). "Chfr expression is downregulated by CpG island hypermethylation in esophageal cancer". Carcinogenesis 23 (10): 1695–9. doi:10.1093/carcin/23.10.1695. PMID 12376479.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Corn PG, Summers MK, Fogt F, et al. (2003). "Frequent hypermethylation of the 5' CpG island of the mitotic stress checkpoint gene Chfr in colorectal and non-small cell lung cancer". Carcinogenesis 24 (1): 47–51. doi:10.1093/carcin/24.1.47. PMID 12538348.
• Toyota M, Sasaki Y, Satoh A, et al. (2003). "Epigenetic inactivation of CHFR in human tumors". Proc. Natl. Acad. Sci. U.S.A. 100 (13): 7818–23. doi:10.1073/pnas.1337066100. PMC 164671. PMID 12810945.
• Bothos J, Summers MK, Venere M, et al. (2003). "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains". Oncogene 22 (46): 7101–7. doi:10.1038/sj.onc.1206831. PMID 14562038.
• Mariatos G, Bothos J, Zacharatos P, et al. (2004). "Inactivating mutations targeting the chfr mitotic checkpoint gene in human lung cancer". Cancer Res. 63 (21): 7185–9. PMID 14612512.
• Shtivelman E (2004). "Promotion of mitosis by activated protein kinase B after DNA damage involves polo-like kinase 1 and checkpoint protein CHFR". Mol. Cancer Res. 1 (13): 959–69. PMID 14638868.
• Bertholon J, Wang Q, Falette N, et al. (2004). "Chfr inactivation is not associated to chromosomal instability in colon cancers". Oncogene 22 (55): 8956–60. doi:10.1038/sj.onc.1207078. PMID 14654793.
• Erson AE, Petty EM (2004). "CHFR-associated early G2/M checkpoint defects in breast cancer cells". Mol. Carcinog. 39 (1): 26–33. doi:10.1002/mc.10161. PMID 14694445.
• Satoh A, Toyota M, Itoh F, et al. (2004). "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer". Cancer Res. 63 (24): 8606–13. PMID 14695171.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Kang HC, Kim IJ, Park JH, et al. (2005). "Promoter hypermethylation and silencing of CHFR mitotic stress checkpoint gene in human gastric cancers". Oncol. Rep. 12 (1): 129–33. doi:10.3892/or.12.1.129. PMID 15201973.
• Matsusaka T, Pines J (2004). "Chfr acts with the p38 stress kinases to block entry to mitosis in mammalian cells". J. Cell Biol. 166 (4): 507–16. doi:10.1083/jcb.200401139. PMC 2172205. PMID 15302856.
• Daniels MJ, Marson A, Venkitaraman AR (2004). "PML bodies control the nuclear dynamics and function of the CHFR mitotic checkpoint protein". Nat. Struct. Mol. Biol. 11 (11): 1114–21. doi:10.1038/nsmb837. PMID 15467728.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.