CHKA

Choline kinase alpha
PDB rendering based on 2cko.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2CKO​, 2CKP​, 2CKQ​, 2I7Q​, 3F2R​, 3G15​, 3ZM9​, 4BR3​, 4CG8​, 4CG9​, 4CGA​, 4DA5​, 5AFV​
Identifiers
Symbols	CHKA ; CHK; CK; CKI; EK
External IDs	OMIM: 118491 MGI: 107760 HomoloGene: 88575 ChEMBL: 3117 GeneCards: CHKA Gene
EC number	2.7.1.32, 2.7.1.82
Gene ontology Molecular function • choline kinase activity • cholinesterase activity • ethanolamine kinase activity • signal transducer activity • ATP binding • drug binding • choline binding • protein homodimerization activity Cellular component • cytosol Biological process • lipid metabolic process • phospholipid metabolic process • phosphatidylethanolamine biosynthetic process • phosphatidylcholine biosynthetic process • CDP-choline pathway • lipid transport • signal transduction • phosphorylation • choline metabolic process • small molecule metabolic process • glycerophospholipid biosynthetic process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1119	12660
Ensembl	ENSG00000110721	ENSMUSG00000024843
UniProt	P35790	O54804
RefSeq (mRNA)	NM_001277	NM_001025566
RefSeq (protein)	NP_001268	NP_001258425
Location (UCSC)	Chr 11: 68.05 – 68.12 Mb	Chr 19: 3.85 – 3.89 Mb
PubMed search

Choline kinase alpha is an enzyme that in humans is encoded by the CHKA gene.^[1][2][3]

The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene.^[3]

In melanocytic cells CHKA gene expression may be regulated by MITF.^[4]

References

1. Hosaka K, Tanaka S, Nikawa J, Yamashita S (Aug 1992). "Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation". FEBS Lett 304 (2–3): 229–32. doi:10.1016/0014-5793(92)80625-Q. PMID 1618328.
2. Aoyama C, Liao H, Ishidate K (Mar 2004). "Structure and function of choline kinase isoforms in mammalian cells". Prog Lipid Res 43 (3): 266–81. doi:10.1016/j.plipres.2003.12.001. PMID 15003397.
3. "Entrez Gene: CHKA choline kinase alpha".
4. Hoek KS, Schlegel NC, Eichhoff OM; et al. (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971.

Further reading

• Spanner S, Ansell GB (1979). "Choline kinase and ethanolamine kinase activity in the cytosol of nerve endings from rat forebrain". Biochem. J. 178 (3): 753–60. PMC 1186576. PMID 36885.
• Spanner S, Ansell GB (1978). "Choline and ethanolamine kinase activity in the cytoplasm of nerve endings from rat forebrain". Adv. Exp. Med. Biol. 101: 237–45. doi:10.1007/978-1-4615-9071-2_23. PMID 208357.
• Kaplan O, Cohen JS (1994). "Lymphocyte activation-31P magnetic resonance studies of energy metabolism and phospholipid pathways". Immunomethods 4 (2): 139–47. doi:10.1006/immu.1994.1015. PMID 8069533.
• Price DJ, Rivnay B, Fu Y; et al. (1997). "Direct association of Csk homologous kinase (CHK) with the diphosphorylated site Tyr568/570 of the activated c-KIT in megakaryocytes". J. Biol. Chem. 272 (9): 5915–20. doi:10.1074/jbc.272.9.5915. PMID 9038210.
• Ross BM, Moszczynska A, Blusztajn JK; et al. (1997). "Phospholipid biosynthetic enzymes in human brain". Lipids 32 (4): 351–8. doi:10.1007/s11745-997-0044-x. PMID 9113621.
• Nakagami K, Uchida T, Ohwada S; et al. (1999). "Increased choline kinase activity and elevated phosphocholine levels in human colon cancer". Jpn. J. Cancer Res. 90 (4): 419–24. doi:10.1111/j.1349-7006.1999.tb00764.x. PMID 10363580.
• Ramírez de Molina A, Penalva V, Lucas L, Lacal JC (2002). "Regulation of choline kinase activity by Ras proteins involves Ral-GDS and PI3K". Oncogene 21 (6): 937–46. doi:10.1038/sj.onc.1205144. PMID 11840339.
• Dubois T, Howell S, Zemlickova E, Aitken A (2002). "Identification of casein kinase Ialpha interacting protein partners". FEBS Lett. 517 (1–3): 167–71. doi:10.1016/S0014-5793(02)02614-5. PMID 12062430.
• Ramírez de Molina A, Gutiérrez R, Ramos MA; et al. (2002). "Increased choline kinase activity in human breast carcinomas: clinical evidence for a potential novel antitumor strategy". Oncogene 21 (27): 4317–22. doi:10.1038/sj.onc.1205556. PMID 12082619.
• Ramírez de Molina A, Rodríguez-González A, Gutiérrez R; et al. (2002). "Overexpression of choline kinase is a frequent feature in human tumor-derived cell lines and in lung, prostate, and colorectal human cancers". Biochem. Biophys. Res. Commun. 296 (3): 580–3. doi:10.1016/S0006-291X(02)00920-8. PMID 12176020.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Gallego-Ortega D, Ramirez De Molina A, Gutierrez R; et al. (2006). "Generation and characterization of monoclonal antibodies against choline kinase alpha and their potential use as diagnostic tools in cancer". Int. J. Oncol. 29 (2): 335–40. doi:10.3892/ijo.29.2.335. PMID 16820874.
• Enaw JO, Zhu H, Yang W; et al. (2007). "CHKA and PCYT1A gene polymorphisms, choline intake and spina bifida risk in a California population". BMC medicine 4: 36. doi:10.1186/1741-7015-4-36. PMC 1770928. PMID 17184542.