CIR (gene)

Corepressor interacting with RBPJ, 1
Identifiers
Symbols	CIR1 ; CIR
External IDs	OMIM: 605228 MGI: 1914185 HomoloGene: 3589 GeneCards: CIR1 Gene
Gene ontology Molecular function • transcription factor activity, sequence-specific DNA binding • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding • transcription corepressor activity • protein binding Cellular component • histone deacetylase complex • nucleus • cytoplasm • microtubule organizing center • nuclear speck Biological process • transcription, DNA-templated • regulation of transcription from RNA polymerase II promoter • mRNA processing • RNA splicing • negative regulation of transcription, DNA-templated Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9541	66935
Ensembl	ENSG00000138433	ENSMUSG00000041777
UniProt	Q86X95	Q9DA19
RefSeq (mRNA)	NM_004882	NM_025854
RefSeq (protein)	NP_004873	NP_080130
Location (UCSC)	Chr 2: 174.35 – 174.4 Mb	Chr 2: 73.28 – 73.31 Mb
PubMed search

Corepressor interacting with RBPJ 1 is a protein that in humans is encoded by the CIR1 gene.^[1][2][3][4]

Interactions

CIR (gene) has been shown to interact with SNW1.^[5]

Model organisms

Model organisms have been used in the study of CIR1 function. A conditional knockout mouse line called Cir1^{tm3a(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[6] Male and female animals underwent a standardized phenotypic screen^[7] to determine the effects of deletion.^{[8][9][10][11]} Additional screens performed: - In-depth immunological phenotyping^[12] - in-depth bone and cartilage phenotyping^[13]

Cir1 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[7][12][13]
Peripheral blood leukocytes 6 Weeks	Normal
Insulin	Normal
Haematology 6 Weeks	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

References

1. Maita H, Kitaura H, Ariga H, Iguchi-Ariga SM (Feb 2005). "CIR, a corepressor of CBF1, binds to PAP-1 and effects alternative splicing". Experimental Cell Research 303 (2): 375–87. doi:10.1016/j.yexcr.2004.10.012. PMID 15652350.
2. Zhang J, Chen H, Weinmaster G, Hayward SD (Mar 2001). "Epstein-Barr virus BamHi-a rightward transcript-encoded RPMS protein interacts with the CBF1-associated corepressor CIR to negatively regulate the activity of EBNA2 and NotchIC". Journal of Virology 75 (6): 2946–56. doi:10.1128/JVI.75.6.2946-2956.2001. PMC 115921. PMID 11222720.
3. Hsieh JJ, Zhou S, Chen L, Young DB, Hayward SD (Jan 1999). "CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex". Proceedings of the National Academy of Sciences of the United States of America 96 (1): 23–8. doi:10.1073/pnas.96.1.23. PMC 15086. PMID 9874765.
4. "Entrez Gene: CIR CBF1 interacting corepressor".
5. Zhou S, Fujimuro M, Hsieh JJ, Chen L, Hayward SD (Feb 2000). "A role for SKIP in EBNA2 activation of CBF1-repressed promoters". Journal of Virology 74 (4): 1939–47. doi:10.1128/JVI.74.4.1939-1947.2000. PMC 111672. PMID 10644367.
6. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88. doi:10.1111/j.1755-3768.2010.4142.x.
7. "International Mouse Phenotyping Consortium".
8. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
9. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
10. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
11. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
12. "Infection and Immunity Immunophenotyping (3i) Consortium".
13. "OBCD Consortium".

Further reading

• DuBridge RB, Tang P, Hsia HC, Leong PM, Miller JH, Calos MP (Jan 1987). "Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system". Molecular and Cellular Biology 7 (1): 379–87. doi:10.1128/mcb.7.1.379. PMC 365079. PMID 3031469.
• Zhou S, Fujimuro M, Hsieh JJ, Chen L, Hayward SD (Feb 2000). "A role for SKIP in EBNA2 activation of CBF1-repressed promoters". Journal of Virology 74 (4): 1939–47. doi:10.1128/JVI.74.4.1939-1947.2000. PMC 111672. PMID 10644367.
• Krithivas A, Young DB, Liao G, Greene D, Hayward SD (Oct 2000). "Human herpesvirus 8 LANA interacts with proteins of the mSin3 corepressor complex and negatively regulates Epstein-Barr virus gene expression in dually infected PEL cells". Journal of Virology 74 (20): 9637–45. doi:10.1128/JVI.74.20.9637-9645.2000. PMC 112396. PMID 11000236.
• Zhou S, Hayward SD (Sep 2001). "Nuclear localization of CBF1 is regulated by interactions with the SMRT corepressor complex". Molecular and Cellular Biology 21 (18): 6222–32. doi:10.1128/MCB.21.18.6222-6232.2001. PMC 87339. PMID 11509665.
• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (Oct 2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Research 11 (10): 1758–65. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.