CNOT1

CCR4-NOT transcription complex, subunit 1
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4C0D​, 4CQO​, 4CRU​, 4CRV​, 4CRW​, 4CT4​, 4CT6​, 4CT7​, 4GMJ​, 4GML​, 4J8S​
Identifiers
Symbols	CNOT1 ; AD-005; CDC39; NOT1; NOT1H
External IDs	OMIM: 604917 MGI: 2442402 HomoloGene: 9453 GeneCards: CNOT1 Gene
Gene ontology Molecular function • poly(A)-specific ribonuclease activity • protein binding • protein domain specific binding • estrogen receptor binding • protein complex scaffold • retinoic acid receptor binding • poly(A) RNA binding • armadillo repeat domain binding Cellular component • cytoplasmic mRNA processing body • extracellular space • nucleoplasm • peroxisomal membrane • cytosol • membrane • CCR4-NOT complex Biological process • negative regulation of transcription from RNA polymerase II promoter • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • nuclear-transcribed mRNA poly(A) tail shortening • transcription, DNA-templated • multicellular organismal development • gene expression • positive regulation of cytoplasmic mRNA processing body assembly • negative regulation of translation • negative regulation of intracellular estrogen receptor signaling pathway • gene silencing by miRNA • negative regulation of retinoic acid receptor signaling pathway • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening • positive regulation of mRNA catabolic process • RNA phosphodiester bond hydrolysis, exonucleolytic • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • regulation of stem cell maintenance Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	23019	234594
Ensembl	ENSG00000125107	ENSMUSG00000036550
UniProt	A5YKK6	Q6ZQ08
RefSeq (mRNA)	NM_001265612	NM_001205226
RefSeq (protein)	NP_001252541	NP_001192155
Location (UCSC)	Chr 16: 58.52 – 58.63 Mb	Chr 8: 95.72 – 95.81 Mb
PubMed search

CCR4-Not complex component, Not1
Identifiers
Symbol	Not1
Pfam	PF04054
InterPro	IPR007196
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

CCR4-NOT transcription complex subunit 1 is a protein that in humans is encoded by the CNOT1 gene.^[1][2]

It is a part of the Ccr4-Not complex, which deadenylates mRNAs.^[3] CNOT1 acts as a scaffold protein, binding other subunits of the complex.^[4][5][6]

Interactions

CNOT1 has been shown to interact with CNOT8.^[7][8]

References

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S (December 2003). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. Check date values in: |year= / |date= mismatch (help)
2. "Entrez Gene: CNOT1 CCR4-NOT transcription complex, subunit 1".
3. Garneau NL, Wilusz J, Wilusz CJ (Feb 2007). "The highways and byways of mRNA decay". Nat. Rev. Mol. Cell Biol. 8 (2): 113–26. doi:10.1038/nrm2104. PMID 17245413.
4. Sandler H, Kreth J, Timmers HT, Stoecklin G (May 2011). "Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin". Nucleic Acids Res. 39 (10): 4373–86. doi:10.1093/nar/gkr011. PMC 3105394. PMID 21278420.
5. Petit AP, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O (Nov 2012). "The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex". Nucleic Acids Res. 40 (21): 11058–72. doi:10.1093/nar/gks883. PMC 3510486. PMID 22977175.
6. Basquin J, Roudko VV, Rode M, Basquin C, Séraphin B, Conti E (Oct 26, 2012). "Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction". Mol. Cell 48 (2): 207–18. doi:10.1016/j.molcel.2012.08.014. PMID 22959269.
7. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
8. Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT (February 2000). "Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits". Nucleic Acids Res. 28 (3): 809–17. doi:10.1093/nar/28.3.809. PMC 102560. PMID 10637334.

External links

• Eukaryotic Linear Motif resource motif class LIG_CNOT1_NIM_1

Further reading

• Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (1): 63–70. doi:10.1093/dnares/6.1.63. PMID 10231032.
• Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT (2000). "Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits". Nucleic Acids Res. 28 (3): 809–17. doi:10.1093/nar/28.3.809. PMC 102560. PMID 10637334.
• Chen J, Rappsilber J, Chiang YC, Russell P, Mann M, Denis CL (2001). "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex". J. Mol. Biol. 314 (4): 683–94. doi:10.1006/jmbi.2001.5162. PMID 11733989.
• Laveder P, De Pittà C, Toppo S, Valle G, Lanfranchi G (2002). "A two-step strategy for constructing specifically self-subtracted cDNA libraries". Nucleic Acids Res. 30 (9): e38. doi:10.1093/nar/30.9.e38. PMC 113861. PMID 11972353.
• Winkler GS, Mulder KW, Bardwell VJ, Kalkhoven E, Timmers HT (2006). "Human Ccr4-Not complex is a ligand-dependent repressor of nuclear receptor-mediated transcription". EMBO J. 25 (13): 3089–99. doi:10.1038/sj.emboj.7601194. PMC 1500986. PMID 16778766.