CNOT7

CCR4-NOT transcription complex, subunit 7
PDB rendering based on 2d5r.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2D5R​, 4GMJ​
Identifiers
Symbols	CNOT7 ; CAF1; Caf1a; hCAF-1
External IDs	OMIM: 604913 MGI: 1298230 HomoloGene: 49011 GeneCards: CNOT7 Gene
EC number	3.1.13.4
Gene ontology Molecular function • 3'-5'-exoribonuclease activity • transcription factor activity, sequence-specific DNA binding • RNA binding • exoribonuclease activity • poly(A)-specific ribonuclease activity • signal transducer activity • protein binding • metal ion binding Cellular component • cytoplasmic mRNA processing body • nucleus • cytosol • membrane • CCR4-NOT complex Biological process • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • nuclear-transcribed mRNA poly(A) tail shortening • deadenylation-dependent decapping of nuclear-transcribed mRNA • carbohydrate metabolic process • transcription, DNA-templated • regulation of translation • signal transduction • positive regulation of cell proliferation • negative regulation of cell proliferation • gene expression • negative regulation of gene expression • gene silencing by RNA • cytoplasmic mRNA processing body assembly • gene silencing by miRNA • exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay • positive regulation of transcription from RNA polymerase II promoter • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening • positive regulation of mRNA catabolic process • RNA phosphodiester bond hydrolysis, exonucleolytic • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	29883	18983
Ensembl	ENSG00000198791	ENSMUSG00000031601
UniProt	Q9UIV1	Q60809
RefSeq (mRNA)	NM_013354	NM_001271542
RefSeq (protein)	NP_037486	NP_001258471
Location (UCSC)	Chr 8: 17.22 – 17.25 Mb	Chr 8: 40.49 – 40.52 Mb
PubMed search

CCR4-NOT transcription complex subunit 7 is a protein that in humans is encoded by the CNOT7 gene.^[1][2][3][4]

Function

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.^[4]

Interactions

CNOT7 has been shown to interact with:

• BTG1,^[5][6]
• PABPC1,^[7]
• TOB1,^[7][8] and
• TOB2.^[8]

References

1. Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT (March 2000). "Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits". Nucleic Acids Res. 28 (3): 809–17. doi:10.1093/nar/28.3.809. PMC 102560. PMID 10637334.
2. Adams MD, Dubnick M, Kerlavage AR, Moreno R, Kelley JM, Utterback TR, Nagle JW, Fields C, Venter JC (March 1992). "Sequence identification of 2,375 human brain genes". Nature 355 (6361): 632–4. doi:10.1038/355632a0. PMID 1538749.
3. Robin-Lespinasse Y, Sentis S, Kolytcheff C, Rostan MC, Corbo L, Le Romancer M (February 2007). "hCAF1, a new regulator of PRMT1-dependent arginine methylation". J. Cell. Sci. 120 (Pt 4): 638–47. doi:10.1242/jcs.03357. PMID 17264152.
4. "Entrez Gene: CNOT7 CCR4-NOT transcription complex, subunit 7".
5. Bogdan JA, Adams-Burton C, Pedicord DL, Sukovich DA, Benfield PA, Corjay MH, Stoltenborg JK, Dicker IB (December 1998). "Human carbon catabolite repressor protein (CCR4)-associative factor 1: cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1". Biochem. J. 336 ( Pt 2): 471–81. PMC 1219893. PMID 9820826.
6. Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, Corbo L (March 2001). "Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway". J. Biol. Chem. 276 (13): 9640–8. doi:10.1074/jbc.M008201200. PMID 11136725.
7. Funakoshi Y, Doi Y, Hosoda N, Uchida N, Osawa M, Shimada I, Tsujimoto M, Suzuki T, Katada T, Hoshino S (December 2007). "Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases". Genes Dev. 21 (23): 3135–48. doi:10.1101/gad.1597707. PMC 2081979. PMID 18056425.
8. Ikematsu N, Yoshida Y, Kawamura-Tsuzuku J, Ohsugi M, Onda M, Hirai M, Fujimoto J, Yamamoto T (December 1999). "Tob2, a novel anti-proliferative Tob/BTG1 family member, associates with a component of the CCR4 transcriptional regulatory complex capable of binding cyclin-dependent kinases". Oncogene 18 (52): 7432–41. doi:10.1038/sj.onc.1203193. PMID 10602502.

Further reading

• Draper MP, Salvadore C, Denis CL (1995). "Identification of a mouse protein whose homolog in Saccharomyces cerevisiae is a component of the CCR4 transcriptional regulatory complex". Mol. Cell. Biol. 15 (7): 3487–95. PMC 230585. PMID 7791755.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M (1996). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi:10.1101/gr.6.9.807. PMID 8889549.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Rouault JP, Prévôt D, Berthet C, Birot AM, Billaud M, Magaud JP, Corbo L (1998). "Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex". J. Biol. Chem. 273 (35): 22563–9. doi:10.1074/jbc.273.35.22563. PMID 9712883.
• Bogdan JA, Adams-Burton C, Pedicord DL, Sukovich DA, Benfield PA, Corjay MH, Stoltenborg JK, Dicker IB (1998). "Human carbon catabolite repressor protein (CCR4)-associative factor 1: cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1". Biochem. J. 336 ( Pt 2) (2): 471–81. PMC 1219893. PMID 9820826.
• Ikematsu N, Yoshida Y, Kawamura-Tsuzuku J, Ohsugi M, Onda M, Hirai M, Fujimoto J, Yamamoto T (1999). "Tob2, a novel anti-proliferative Tob/BTG1 family member, associates with a component of the CCR4 transcriptional regulatory complex capable of binding cyclin-dependent kinases". Oncogene 18 (52): 7432–41. doi:10.1038/sj.onc.1203193. PMID 10602502.
• Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, Corbo L (2001). "Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway". J. Biol. Chem. 276 (13): 9640–8. doi:10.1074/jbc.M008201200. PMID 11136725.
• Yoshida Y, Hosoda E, Nakamura T, Yamamoto T (2001). "Association of ANA, a member of the antiproliferative Tob family proteins, with a Caf1 component of the CCR4 transcriptional regulatory complex". Jpn. J. Cancer Res. 92 (6): 592–6. doi:10.1111/j.1349-7006.2001.tb01135.x. PMID 11429045.
• Flanagan J, Healey S, Young J, Whitehall V, Chenevix-Trench G (2003). "Analysis of the transcription regulator, CNOT7, as a candidate chromosome 8 tumor suppressor gene in colorectal cancer". Int. J. Cancer 106 (4): 505–9. doi:10.1002/ijc.11264. PMID 12845644.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.