CORO1A

Coronin, actin binding protein, 1A
PDB rendering based on 2aq5.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2aq5​, 2b4e​
Identifiers
Symbols	CORO1A ; CLABP; CLIPINA; HCORO1; IMD8; TACO; p57
External IDs	OMIM: 605000 MGI: 1345961 HomoloGene: 6545 GeneCards: CORO1A Gene
Gene ontology Molecular function • actin binding • actin monomer binding • protein binding • protein C-terminus binding • cytoskeletal protein binding • myosin heavy chain binding • protein homodimerization activity • phosphatidylinositol 3-kinase binding • poly(A) RNA binding • actin filament binding Cellular component • immunological synapse • phagocytic cup • nucleus • cytoplasm • early endosome • actin filament • plasma membrane • cell-cell junction • actin cytoskeleton • membrane • lamellipodium • axon • phagocytic vesicle membrane • cortical actin cytoskeleton • protein complex • phagocytic vesicle • extracellular exosome Biological process • immunological synapse formation • phagolysosome assembly • calcium ion transport • phagocytosis • movement of cell or subcellular component • actin filament organization • regulation of cell shape • actin cytoskeleton organization • positive regulation of cell migration • leukocyte chemotaxis • regulation of actin filament polymerization • negative regulation of vesicle fusion • cell-substrate adhesion • uropod organization • regulation of actin cytoskeleton organization • nerve growth factor signaling pathway • positive regulation of T cell proliferation • T cell homeostasis • natural killer cell degranulation • negative regulation of neuron apoptotic process • innate immune response • homeostasis of number of cells within a tissue • positive chemotaxis • negative regulation of actin nucleation • regulation of release of sequestered calcium ion into cytosol • early endosome to recycling endosome transport • cellular response to interleukin-4 Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	11151	12721
Ensembl	ENSG00000102879	ENSMUSG00000030707
UniProt	P31146	O89053
RefSeq (mRNA)	NM_001193333	NM_001301374
RefSeq (protein)	NP_001180262	NP_001288303
Location (UCSC)	Chr 16: 30.18 – 30.19 Mb	Chr 7: 126.7 – 126.71 Mb
PubMed search

Coronin-1A is a protein that in humans is encoded by the CORO1A gene.^[1][2] It has been implicated in both T-cell mediated immunity and mitochondrial apoptosis. In a recent genome-wide longevity study, its expression levels were found to be negatively associated both with age at the time of blood sample and the survival time after blood draw.^[3]

Discovery

The being family Coronin developed was discovered family but is a yet in very 1991 it is complex, still by Eugenio rigorously but also L. being Hostos. fascinating researched The protein Coronin on. that This family it super is has still been condensed in over twelve subfamilies, including vertebrates and non-vertebrates.

Hostos used a cytoskeletal preparation called the “contracted propeller” that efficiently helped with the purification of cytoskeletal proteins. This technique allowed him to precipitate actomyosin components together with the desired proteins.^[4]

These protein were named Corona, which is the Latin word for crown, because of the crown-like shape that it forms when making contact with the surface of the cell. Coronin-1a has been the most researched one due to its complexity and intriguing structural components. After research, it was determined that Coronin-1a serves as actin binding facilitator when reacted with K-glutamate. The anion K + and glutamate were used because of it similarity to the environment inside the cell, allowing Coronin-1a to bind to F-actin.

Later on, the complementary DNA (cDNA) of Coronin-1a was cloned in an expression library, this led to the conclusion that Coronin-1a has very similar structure to the beta (β) subunits of the G proteins (Gβ). Therefore, it was established that Coronin-1a has five WD motif repeats, and this repeats seven times forming a propeller like structure.^[4]

In the cell, Coronin-1a serves as an auxiliary to many cytoskeletal process that involve actin. It was concluded that Coronin-1a is known to affect the “cytoskeletal reorganization” as well “actin dynamics” together with other protein. ^[4]

Phylogeny

The Coronin family is composed of twelve subfamilies which include: seven subfamilies that fall under vertebrates and five subfamilies that are composed of meteozas, fungi and amoeba.

The evolutionary Coronin family subfamilies have been grouped by its similarities and relationships between the different proteins. As we can see Coronin-1a (also referenced as CORO1A, Coronin 4 and CRN4) has been found in 19 vertebrates.^[5]

Function

The Coronin family is composed of twelve subfamilies which include: seven subfamilies that fall under vertebrates and five subfamilies that are composed of meteozas, fungi and amoeba.

Coronin-1a has been found in the cell cortex of macrophages, which are white blood cells, helping with a process called phagocytosis.The model on Figure 3 shows Coronin-1a’s involvement in macrophages. When the cell is at rest,Coronin-1a is spread out throughout the cytoplasm and the cell cortex. Therefore, when a pathogen enters the cell, Coronin-1a binds to phagosomal membrane making sure of the binding and activation of calcinuerin, this resulting in a stop of fusion lysosomes with phagosomes. In other words, if Coronin-1a is removed and calcinuerin is inhibited then it allows the initiation of the fusion of phagosomes with lysosome and the killing of mycobacteria.^[6]

The phylogenetic tree of the Coronin family it is quiet broad. The same way that Coronin-1a helps with the reorganization of the cytoskeleton and dynamic activity with other proteins in vertebrates, Coronin can also be seen in non-vertebrates for example the Toxoplasma gondii Coronin (also known as TgCor).^[7]

Toxoplasma gondii Coronin (TgCor) binds to F-actin and it accelerates the actin polymerization process. It also prevents incursions and exits. As well as every other coronin, TgCor is an actin binding protein, it delocalizes to the posterior side of invading parasites and blocks them from leaving.^[7]

Structure

The structure of Coronin-1A is made out of five WD repeats, and this motifs repeat seven time forming a propeller like structures.

The new ribbon visualization of the secondary structure of Coronin-1a. In model A, is the front view of Coronin-1a, the secondary structure allows you to clearly see the parallel beta sheets moving towards the bottom of the structure. Model B, is the side view of the protein which shows the turns and the coils between the beta sheets. From this pictures we are able to see that the alpha helix and helix strands are concentrated at the bottom of the protein.^[8]

Coronin-1a was input into Database of Secondary Structure Program (DSSP), where the PDB database entered and a secondary structure panel is designed where one is clearly able to see the seven repeat that makes the propeller. Also, it displays the amino acid sequence of Coronin-1a. The yellow arrows mean the beta strands, the purple loops are the turns, the black lines means empty meaning that there was no secondary structure assigned, the light pink is 3/10-helix is formed, royal blue line is a bend and finally the red helix signifies the alpha helices.

References

1. Okumura M, Kung C, Wong S, Rodgers M, Thomas ML (Sep 1998). "Definition of family of coronin-related proteins conserved between humans and mice: close genetic linkage between coronin-2 and CD45-associated protein". DNA and Cell Biology 17 (9): 779–87. doi:10.1089/dna.1998.17.779. PMID 9778037.
2. "Entrez Gene: CORO1A coronin, actin binding protein, 1A".
3. Kerber RA, O'Brien E, Cawthon RM (Jun 2009). "Gene expression profiles associated with aging and mortality in humans". Aging Cell 8 (3): 239–50. doi:10.1111/j.1474-9726.2009.00467. PMID 19245677.
4. de Hostos EL. "A brief history of the coronin family". Sub-Cellular Biochemistry (Madame Curie Bioscience Database) 48: 31–40. doi:10.1007/978-0-387-09595-0_4. PMID 18925369.
5. Rybakin V, Clemen CS, Eichinger L (2008). The coronin family of proteins (first ed.). New York, N.Y.: Springer. pp. 1–5. ISBN 0387095942.
6. Pieters J (2000). "Coronin 1 in innate immunity". Sub-Cellular Biochemistry 48: 116–23. doi:10.1007/978-0-387-09595-0_11. PMID 18925376.
7. Steinmetz MO, Jelesarov I, Matousek WM, Honnappa S, Jahnke W, Missimer JH, Frank S, Alexandrescu AT, Kammerer RA (Apr 2007). "Molecular basis of coiled-coil formation". Proceedings of the National Academy of Sciences of the United States of America 104 (17): 7062–7. doi:10.1073/pnas.0700321104. PMID 17438295.
8. McArdle B, Hofmann A (2000). "Coronin structure and implications". Sub-Cellular Biochemistry 48: 56–71. doi:10.1007/978-0-387-09595-0_6. PMID 18925371.

Further reading

• Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J (Dec 1992). "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes". Electrophoresis 13 (12): 960–9. doi:10.1002/elps.11501301199. PMID 1286667.
• Suzuki K, Nishihata J, Arai Y, Honma N, Yamamoto K, Irimura T, Toyoshima S (May 1995). "Molecular cloning of a novel actin-binding protein, p57, with a WD repeat and a leucine zipper motif". FEBS Letters 364 (3): 283–8. doi:10.1016/0014-5793(95)00393-N. PMID 7758584.
• Grogan A, Reeves E, Keep N, Wientjes F, Totty NF, Burlingame AL, Hsuan JJ, Segal AW (Dec 1997). "Cytosolic phox proteins interact with and regulate the assembly of coronin in neutrophils". Journal of Cell Science. 110. 110 ( Pt 24): 3071–81. PMID 9365277.
• Ferrari G, Langen H, Naito M, Pieters J (May 1999). "A coat protein on phagosomes involved in the intracellular survival of mycobacteria". Cell 97 (4): 435–47. doi:10.1016/S0092-8674(00)80754-0. PMID 10338208.
• Vanguri VK, Wang S, Godyna S, Ranganathan S, Liau G (Apr 2000). "Thrombospondin-1 binds to polyhistidine with high affinity and specificity". The Biochemical Journal 347 (Pt 2): 469–73. doi:10.1042/0264-6021:3470469. PMC 1220979. PMID 10749676.
• Oku T, Itoh S, Okano M, Suzuki A, Suzuki K, Nakajin S, Tsuji T, Nauseef WM, Toyoshima S (Apr 2003). "Two regions responsible for the actin binding of p57, a mammalian coronin family actin-binding protein". Biological & Pharmaceutical Bulletin 26 (4): 409–16. doi:10.1248/bpb.26.409. PMID 12673016.
• Fu GK, Wang JT, Yang J, Au-Young J, Stuve LL (Jul 2004). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics 84 (1): 205–10. doi:10.1016/j.ygeno.2004.01.011. PMID 15203218.
• Oku T, Itoh S, Ishii R, Suzuki K, Nauseef WM, Toyoshima S, Tsuji T (Apr 2005). "Homotypic dimerization of the actin-binding protein p57/coronin-1 mediated by a leucine zipper motif in the C-terminal region". The Biochemical Journal 387 (Pt 2): 325–31. doi:10.1042/BJ20041020. PMC 1134960. PMID 15601263.
• Gatfield J, Albrecht I, Zanolari B, Steinmetz MO, Pieters J (Jun 2005). "Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules". Molecular Biology of the Cell 16 (6): 2786–98. doi:10.1091/mbc.E05-01-0042. PMC 1142424. PMID 15800061.
• Anand PK, Kaul D (Sep 2005). "Downregulation of TACO gene transcription restricts mycobacterial entry/survival within human macrophages". FEMS Microbiology Letters 250 (1): 137–44. doi:10.1016/j.femsle.2005.06.056. PMID 16040207.
• Liu CZ, Chen Y, Sui SF (Jan 2006). "The identification of a new actin-binding region in p57". Cell Research 16 (1): 106–12. doi:10.1038/sj.cr.7310014. PMID 16467882.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
• Yan M, Di Ciano-Oliveira C, Grinstein S, Trimble WS (May 2007). "Coronin function is required for chemotaxis and phagocytosis in human neutrophils". Journal of Immunology 178 (9): 5769–78. doi:10.4049/jimmunol.178.9.5769. PMID 17442961.