CPSF6

Cleavage and polyadenylation specific factor 6, 68kDa
Rendering based on PDB 3P5T​.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3P5T​, 3P6Y​, 3Q2S​, 3Q2T​, 4B4N​, 4U0A​, 4U0B​, 4WYM​
Identifiers
Symbols	CPSF6 ; CFIM; CFIM68; HPBRII-4; HPBRII-7
External IDs	OMIM: 604979 MGI: 1913948 HomoloGene: 134126 GeneCards: CPSF6 Gene
Gene ontology Molecular function • nucleotide binding • RNA binding • mRNA binding • protein binding • poly(A) RNA binding Cellular component • nucleus • nucleoplasm • mRNA cleavage factor complex • membrane • ribonucleoprotein complex • paraspeckles Biological process • mRNA polyadenylation • mRNA processing • protein tetramerization Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	11052	432508
Ensembl	ENSG00000111605	ENSMUSG00000055531
UniProt	Q16630	Q6NVF9
RefSeq (mRNA)	NM_001300947	NM_001013391
RefSeq (protein)	NP_001287876	NP_001013409
Location (UCSC)	Chr 12: 69.24 – 69.27 Mb	Chr 10: 117.34 – 117.38 Mb
PubMed search

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene.^[1][2][3]

Function

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitement of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.^[3]

Interactions

CPSF6 has been shown to interact with WWP1^[4] and PLSCR1.^[4]

References

1. Rüegsegger U, Blank D, Keller W (Jan 1998). "Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits". Molecular Cell 1 (2): 243–53. doi:10.1016/S1097-2765(00)80025-8. PMID 9659921.
2. Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino SM (Apr 2007). "Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit". Molecular Biology of the Cell 18 (4): 1282–92. doi:10.1091/mbc.E06-09-0846. PMC 1838998. PMID 17267687.
3. "Entrez Gene: CPSF6 cleavage and polyadenylation specific factor 6, 68kDa".
4. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.

Further reading

• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Rüegsegger U, Beyer K, Keller W (Mar 1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors". The Journal of Biological Chemistry 271 (11): 6107–13. doi:10.1074/jbc.271.11.6107. PMID 8626397.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• de Vries H, Rüegsegger U, Hübner W, Friedlein A, Langen H, Keller W (Nov 2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". The EMBO Journal 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781. PMID 11060040.
• Awasthi S, Alwine JC (Nov 2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA 9 (11): 1400–9. doi:10.1261/rna.5104603. PMC 1287061. PMID 14561889.
• Brown KM, Gilmartin GM (Dec 2003). "A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im". Molecular Cell 12 (6): 1467–76. doi:10.1016/S1097-2765(03)00453-2. PMID 14690600.
• Dettwiler S, Aringhieri C, Cardinale S, Keller W, Barabino SM (Aug 2004). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization". The Journal of Biological Chemistry 279 (34): 35788–97. doi:10.1074/jbc.M403927200. PMID 15169763.
• Venkataraman K, Brown KM, Gilmartin GM (Jun 2005). "Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition". Genes & Development 19 (11): 1315–27. doi:10.1101/gad.1298605. PMC 1142555. PMID 15937220.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Millevoi S, Loulergue C, Dettwiler S, Karaa SZ, Keller W, Antoniou M, Vagner S (Oct 2006). "An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries". The EMBO Journal 25 (20): 4854–64. doi:10.1038/sj.emboj.7601331. PMC 1618107. PMID 17024186.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.