CYB561

Cytochrome b561
Identifiers
Symbols	CYB561 ; CYB561A1; FRRS2
External IDs	OMIM: 600019 MGI: 103253 HomoloGene: 37552 GeneCards: CYB561 Gene
Gene ontology Molecular function • ferric-chelate reductase activity • protein binding • oxidoreductase activity • transmembrane electron transfer carrier • metal ion binding Cellular component • integral component of membrane Biological process • electron transport chain • transmembrane transport • oxidation-reduction process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1534	13056
Ensembl	ENSG00000008283	ENSMUSG00000019590
UniProt	P49447	Q60720
RefSeq (mRNA)	NM_001017916	NM_007805
RefSeq (protein)	NP_001017916	NP_031831
Location (UCSC)	Chr 17: 63.43 – 63.45 Mb	Chr 11: 105.93 – 105.95 Mb
PubMed search

Cytochrome b561 is a protein that in humans is encoded by the CYB561 gene.^[1][2]

Model organisms

Cyb561 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal[3]
Indirect calorimetry	Normal
Glucose tolerance test	Abnormal[4]
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Eye Histopathology	Normal
Salmonella infection	Normal[5]
Citrobacter infection	Normal[6]
All tests and analysis from[7][8]

Model organisms have been used in the study of CYB561 function. A conditional knockout mouse line, called Cyb561^{tm1a(EUCOMM)Wtsi[9][10]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.^[11][12][13]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[7][14] Twenty three tests were carried out on mutant mice and one significant abnormality was observed: female homozygotes displayed a decreased circulating glucose level after a glucose tolerance test.^[7]

References

1. McBride OW, Yi HF, Srivastava M (Nov 1994). "The human cytochrome b561 gene (CYB561) is located at 17q11-qter". Genomics 21 (3): 662–3. doi:10.1006/geno.1994.1332. PMID 7959749.
2. "Entrez Gene: CYB561 cytochrome b-561".
3. "Dysmorphology data for Cyb561". Wellcome Trust Sanger Institute.
4. "Glucose tolerance test data for Cyb561". Wellcome Trust Sanger Institute.
5. "Salmonella infection data for Cyb561". Wellcome Trust Sanger Institute.
6. "Citrobacter infection data for Cyb561". Wellcome Trust Sanger Institute.
7. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
8. Mouse Resources Portal, Wellcome Trust Sanger Institute.
9. "International Knockout Mouse Consortium".
10. "Mouse Genome Informatics".
11. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
12. Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
13. Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
14. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Duong LT, Fleming PJ (1982). "Isolation and properties of cytochrome b561 from bovine adrenal chromaffin granules.". J. Biol. Chem. 257 (15): 8561–4. PMID 7096323.
• Srivastava M (1995). "Genomic structure and expression of the human gene encoding cytochrome b561, an integral protein of the chromaffin granule membrane.". J. Biol. Chem. 270 (39): 22714–20. doi:10.1074/jbc.270.39.22714. PMID 7559396.
• Srivastava M, Gibson KR, Pollard HB, Fleming PJ (1994). "Human cytochrome b561: a revised hypothesis for conformation in membranes which reconciles sequence and functional information". Biochem. J. 303 (Pt 3): 915–21. PMC 1137633. PMID 7980462.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Bashtovyy D, Bérczi A, Asard H, Páli T (2004). "Structure prediction for the di-heme cytochrome b561 protein family". Protoplasma 221 (1–2): 31–40. doi:10.1007/s00709-002-0065-0. PMID 12768339.
• Kang MK, Kameta A, Shin KH; et al. (2003). "Senescence-associated genes in normal human oral keratinocytes". Exp. Cell Res. 287 (2): 272–81. doi:10.1016/S0014-4827(03)00061-2. PMID 12837283.
• Vargas JD, Herpers B, McKie AT; et al. (2003). "Stromal cell-derived receptor 2 and cytochrome b561 are functional ferric reductases". Biochim. Biophys. Acta 1651 (1–2): 116–23. doi:10.1016/S1570-9639(03)00242-5. PMID 14499595.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.