Charles Best
Charles Best may refer to:
- Charles Best (poet) (1570–1627), English poet
- Charles Best (army officer), a British army office who served in the armies of the East India Company, Britain and Hanover from 1781 until the end of the Napoleonic Wars
- Charles Herbert Best (1899–1978), medical scientist; co-discoverer of insulin
- Charles Best (politician) (1909–1996), Australian politician
- Charles Alexander Best (1931–1978), Canadian politician, son of Charles Herbert Best
- Charles Best (American football), American college football coach
- Charles Best (businessman), founder of Donorschoose.org
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