Chemical shift

In nuclear magnetic resonance (NMR) spectroscopy, the chemical shift is the resonant frequency of a nucleus relative to a standard in a magnetic field. Often the position and number of chemical shifts are diagnostic of the structure of a molecule.[1][2][3] Chemical shifts are also used to describe signals in other forms of spectroscopy such as photoemission spectroscopy.

Some atomic nuclei possess a magnetic moment (nuclear spin), which gives rise to different energy levels and resonance frequencies in a magnetic field. The total magnetic field experienced by a nucleus includes local magnetic fields induced by currents of electrons in the molecular orbitals (note that electrons have a magnetic moment themselves). The electron distribution of the same type of nucleus (e.g. 1H, 13C, 15N) usually varies according to the local geometry (binding partners, bond lengths, angles between bonds, ...), and with it the local magnetic field at each nucleus. This is reflected in the spin energy levels (and resonance frequencies). The variations of nuclear magnetic resonance frequencies of the same kind of nucleus, due to variations in the electron distribution, is called the chemical shift. The size of the chemical shift is given with respect to a reference frequency or reference sample (see also chemical shift referencing), usually a molecule with a barely distorted electron distribution.

Operating frequency

The operating (or Larmor) frequency \omega_0 of a magnet is calculated from the Larmor equation[4]

\omega_{0} = \gamma B_0\,

where B_0 is the actual strength of the magnet in units like teslas or gauss, and \gamma is the gyromagnetic ratio of the nucleus being tested which is in turn calculated from its magnetic moment \mu and spin number I with the nuclear magneton \mu_N and the Planck constant h:

\gamma = \frac{\mu\,\mu_N}{hI}\,

Thus, the proton operating frequency for a 1 T magnet is calculated as:

\omega _0 = \gamma B_0 = \frac{{2.79 \times 5.05 \times 10^{ - 27}\,{\rm{J/T}} }}{{6.62 \times 10^{ - 34}\,{\rm{Js}} \times \left( {1/2} \right)}} \times 1\,{\rm{T}} = 42.5\,{\rm{MHz}} \,

Chemical shift referencing

Chemical shift δ is usually expressed in parts per million (ppm) by frequency, because it is calculated from

\delta = {( \nu_{sample} - \nu_{reference} ) \over \nu_{reference}}[5]

where \nu_{sample} is the absolute resonance frequency of the sample and \nu_{reference} is the absolute resonance frequency of a standard reference compound, measured in the same applied magnetic field (B_0). Since the numerator is usually expressed in hertz, and the denominator in megahertz, delta is expressed in ppm.

The detected frequencies (in Hz) for 1H, 13C, and 29Si nuclei are usually referenced against TMS (tetramethylsilane) or DSS, which by the definition above have a chemical shift of zero if chosen as the reference. Other standard materials are used for setting the chemical shift for other nuclei.

Thus, an NMR signal observed at a frequency 300 Hz higher than the signal from TMS, where the TMS resonance frequency is 300 MHz, has a chemical shift of:

\frac{300\,\rm Hz}{300\times10^6\,\rm Hz}=1\times10^{-6}= 1\,\rm ppm \,

Although the absolute resonance frequency depends on the applied magnetic field, the chemical shift is independent of external magnetic field strength. On the other hand, the resolution of NMR will increase with applied magnetic field.

The induced magnetic field

The electrons around a nucleus will circulate in a magnetic field and create a secondary induced magnetic field. This field opposes the applied field as stipulated by Lenz's law and atoms with higher induced fields (i.e., higher electron density) are therefore called shielded, relative to those with lower electron density. The chemical milieu of an atom can influence its electron density through the polar effect. Electron-donating alkyl groups, for example, lead to increased shielding while electron-withdrawing substituents such as nitro groups lead to deshielding of the nucleus. Not only substituents cause local induced fields. Bonding electrons can also lead to shielding and deshielding effects. A striking example of this are the pi bonds in benzene. Circular current through the hyperconjugated system causes a shielding effect at the molecule's center and a deshielding effect at its edges. Trends in chemical shift are explained based on the degree of shielding or deshielding.

Nuclei are found to resonate in a wide range to the left (or more rare to the right) of the internal standard. When a signal is found with a higher chemical shift:

Conversely a lower chemical shift is called a diamagnetic shift, and is upfield and more shielded.

Diamagnetic shielding

In real molecules protons are surrounded by a cloud of charge due to adjacent bonds and atoms. In an applied magnetic field (B0) electrons circulate and produce an induced field (Bi) which opposes the applied field. The effective field at the nucleus will be B = B0 − Bi. The nucleus is said to be experiencing a diamagnetic shielding.

Factors causing chemical shifts

Important factors influencing chemical shift are electron density, electronegativity of neighboring groups and anisotropic induced magnetic field effects.

Electron density shields a nucleus from the external field. For example, in proton NMR the electron-poor tropylium ion has its protons downfield at 9.17 ppm, those of the electron-rich cyclooctatetraenyl anion move upfield to 6.75 ppm and its dianion even more upfield to 5.56 ppm.

A nucleus in the vicinity of an electronegative atom experiences reduced electron density and the nucleus is therefore deshielded. In proton NMR of methyl halides (CH3X) the chemical shift of the methyl protons increase in the order I < Br < Cl < F from 2.16 ppm to 4.26 ppm reflecting this trend. In carbon NMR the chemical shift of the carbon nuclei increase in the same order from around –10 ppm to 70 ppm. Also when the electronegative atom is removed further away the effect diminishes until it can be observed no longer.

Anisotropic induced magnetic field effects are the result of a local induced magnetic field experienced by a nucleus resulting from circulating electrons that can either be paramagnetic when it is parallel to the applied field or diamagnetic when it is opposed to it. It is observed in alkenes where the double bond is oriented perpendicular to the external field with pi electrons likewise circulating at right angles. The induced magnetic field lines are parallel to the external field at the location of the alkene protons which therefore shift downfield to a 4.5 ppm to 7.5 ppm range. The three-dimensional space where a diamagnetic shift is called the shielding zone with a cone-like shape aligned with the external field.

The protons in aromatic compounds are shifted downfield even further with a signal for benzene at 7.73 ppm as a consequence of a diamagnetic ring current.

Alkyne protons by contrast resonate at high field in a 2–3 ppm range. For alkynes the most effective orientation is the external field in parallel with electrons circulation around the triple bond. In this way the acetylenic protons are located in the cone-shaped shielding zone hence the upfield shift.

Magnetic properties of most common nuclei

1H and 13C aren't the only nuclei susceptible to NMR experiments. A number of different nuclei can also be detected, although the use of such techniques is generally rare due to small relative sensitivities in NMR experiments (compared to 1H) of the nuclei in question, the other factor for rare use being their slender representation in nature/organic compounds.

Magnetic properties of common nuclei[6]
Isotope Occurrence
in nature
(%)		 Spin number I Magnetic moment μ[7]
 Electric quadrupole moment
(e×10−24 cm2)		 Operating frequency at 7 T
(MHz)		 Relative sensitivity
1H 99.984 1/2 2.79628   300.13 1
2H 0.016 1 0.85739 2.8 x 10−3 46.07 0.0964
10B 18.8 3 1.8005 7.4 x 10−2 32.25 0.0199
11B 81.2 3/2 2.6880 2.6 x 10−2 96.29 0.165
12C 98.9 0        
13C 1.1 1/2 0.70220   75.47 0.0159
14N 99.64 1 0.40358 7.1 x 10−2 21.68 0.00101
15N 0.37 1/2 −0.28304   30.41 0.00104
16O 99.76 0        
17O 0.0317 5/2 −1.8930 −4.0 x 10−3 40.69 0.0291
19F 100 1/2 2.6273   282.40 0.834
28Si 92.28 0        
29Si 4.70 1/2 −0.5548   59.63 0.0785
31P 100 1/2 1.1205   121.49 0.0664
35Cl 75.4 3/2 0.92091 −7.9 x 10−2 29.41 0.0047
37Cl 24.6 3/2 0.68330 −6.2 x 10−2 24.48 0.0027

1H, 13C, 15N, 19F and 31P are the five nuclei that have the greatest importance in NMR experiments:

Other chemical shifts

The related Knight shift (first reported in 1949) is observed with pure metals. The NMR chemical shift in its present-day meaning first appeared in journals in 1950. Chemical shifts with a different meaning appear in X-ray photoelectron spectroscopy as the shift in atomic core-level energy due to a specific chemical environment. The term is also used in Mössbauer spectroscopy, where similarly to NMR it refers to a shift in peak position due to the local chemical bonding environment. As is the case for NMR the chemical shift reflects the electron density at the atomic nucleus.[8]

See also

References

  1. ↑ Spectrometric Identification of organic Compounds Silverstein, Bassler, Morrill 4th Ed. ISBN 0-471-09070-0
  2. ↑ Organic Spectroscopy William Kemp 3rd Ed. ISBN 0-333-41767-4
  3. ↑ Basic 1H - 13C-NMR spectroscopy Metin Balei ISBN 0-444-51811-8
  4. ↑ Chemical Shift | NMRCentral
  5. ↑ R.K. Harris, E.D. Becker, S.M. Cabral de Menezes, R. Goodfellow, P. Granger, Pure Appl. Chem., 2001, 73, pp. 1795-1818. DOI: 10.1351/pac200173111795
  6. ↑ CRC Handbook of Chemistry and Physics 65Th Ed
  7. ↑ (units of the nuclear magneton)
  8. ↑ A Short History of Three Chemical Shifts Shin-ichi Nagaoka Vol. 84 No. 5 May 2007 Journal of Chemical Education 801, doi:10.1021/ed084p801

External links

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