Cholesterol-5,6-oxide hydrolase

Cholesterol-5,6-oxide hydrolase
Identifiers
EC number	3.3.2.11
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Cholesterol-5,6-oxide hydrolase (EC 3.3.2.11, cholesterol-epoxide hydrolase, ChEH) is an enzyme with systematic name 5,6alpha-epoxy-5alpha-cholestan-3beta-ol hydrolase.^{[1][2][3][4][5]} This enzyme catalyses the following chemical reaction

(1) 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H₂O $\rightleftharpoons$ 5alpha-cholestane-3beta,5alpha,6beta-triol

(2) 5,6beta-epoxy-5beta-cholestan-3beta-ol + H₂O $\rightleftharpoons$ 5alpha-cholestane-3beta,5alpha,6beta-triol

The enzyme works equally well with either epoxide as substrate.

References

1. Levin, W., Michaud, D.P., Thomas, P.E. and Jerina, D.M. (1983). "Distinct rat hepatic microsomal epoxide hydrolases catalyze the hydration of cholesterol 5,6 α-oxide and certain xenobiotic alkene and arene oxides". Arch. Biochem. Biophys. 220: 485–494. doi:10.1016/0003-9861(83)90439-3. PMID 6401984.
2. Oesch, F., Timms, C.W., Walker, C.H., Guenthner, T.M., Sparrow, A., Watabe, T. and Wolf, C.R. (1984). "Existence of multiple forms of microsomal epoxide hydrolases with radically different substrate specificities". Carcinogenesis 5: 7–9. doi:10.1093/carcin/5.1.7. PMID 6690087.
3. Sevanian, A. and McLeod, L.L. (1986). "Catalytic properties and inhibition of hepatic cholesterol-epoxide hydrolase". J. Biol. Chem. 261: 54–59. PMID 3941086.
4. Fretland, A.J. and Omiecinski, C.J. (2000). "Epoxide hydrolases: biochemistry and molecular biology". Chem. Biol. Interact. 129: 41–59. doi:10.1016/S0009-2797(00)00197-6. PMID 11154734.
5. Newman, J.W., Morisseau, C. and Hammock, B.D. (2005). "Epoxide hydrolases: their roles and interactions with lipid metabolism". Prog. Lipid Res. 44: 1–51. doi:10.1016/j.plipres.2004.10.001. PMID 15748653.

External links

• cholesterol-5,6-oxide hydrolase at the US National Library of Medicine Medical Subject Headings (MeSH)