Coding strand

When referring to DNA transcription, the coding strand is the DNA strand which has the same base sequence as the RNA transcript produced (although with thymine replaced by uracil). It is this strand which contains codons, while the non-coding strand contains anti-codons. During transcription, RNA Pol II binds the non-coding strand, reads the anti-codons, and transcribes their sequence to synthesize an RNA transcript with complementary bases. By convention, the coding strand is the strand used when displaying a DNA sequence. It is presented in the 5' to 3' direction.

Alternative terms for strands

Wherever a gene exists on a DNA molecule, one strand is the coding strand (or sense strand or non-template strand), and the other is the noncoding strand (also called the antisense strand,^[1] anticoding strand, template strand, or transcribed strand).

Strands in transcription bubble

During transcription, RNA polymerase unwinds a short section of the DNA double helix near the start of the gene (the transcription start site). This unwound section is known as the transcription bubble. The RNA polymerase, and with it the transcription bubble, travels along the noncoding strand in the opposite, 3' to 5', direction, as well as polymerizing a newly synthesized strand in 5' to 3' or downstream direction. The DNA double helix is rewound by RNA polymerase at the rear of the transcription bubble (Lewin, pp 235 ^[1]). Like how two adjacent zippers work, when pulled together, they unzip and rezip as they proceed in a particular direction.

RNA-DNA hybrid

Where the helix is unwound, the coding strand consists of unpaired bases, while the template strand consists of an RNA:DNA composite, followed by a number of unpaired bases at the rear. This hybrid consists of the most recently added nucleotides of the RNA transcript, complementary base-paired to the template strand. The number of base-pairs in the hybrid is under investigation, but it has been suggested that the hybrid is formed from the last 10 nucleotides added.^[2]

References

1 2 Lewin, Benjamin (2008). Genes IX. Oxford University Press. p. 129. ISBN 978-0-7637-4063-4.
↑ Griffiths 2005, pp. 259–265

Bibliography

Griffiths, A.J.F.; et al. (2005). Introduction to Genetic Analysis (8th ed.). W.H. Freeman. ISBN 0-7167-4939-4.
Lewin, B. (2000). Genes VII. New York: Oxford University Press. ISBN 0-19-879277-8.

This article is issued from Wikipedia - version of the Thursday, March 10, 2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.