Spin column-based nucleic acid purification

Silica in a spin column with water and with DNA sample in chaotropic buffer

Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions.

The stages of the method are:[1][2]

Even prior to the major techniques employed today it was known that in the presence of chaotropic agents, such as sodium iodide or sodium perchlorate, DNA binds to silica, glass particles or to unicellular algae called diatoms which shield their cell walls with silica. This property was used to purify nucleic acid using glass powder or silica beads under alkaline conditions. [3] This was later improved used guanidinium thiocyanate or guanidinium hydrochloride as the chaotropic agent.[4] The use of glass beads was later changed to silica gel.

See also

References

  1. Matson, Robert S. (2008). Microarray Methods and Protocols. Boca Raton, Florida: CRC. pp. 27–29. ISBN 1420046659.
  2. Kumar, Anil (2006). Genetic Engineering. New York: Nova Science Publishers. pp. 101–102. ISBN 159454753X.
  3. Marko MA, Chipperfield R, Birnboim HC. A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder. Anal Biochem. 1982 Apr;121(2):382-7. PMID 6179438
  4. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495-503. PMID 1691208
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