Cph1 holin family

The Cph1 Holin Family (TC# 1.E.16) is also called the CDD Holin 4 superfamily, but belongs to the Holin Superfamily IV as classified in the Transporter Classification Database (TCDB).[1] A representative list of members belonging to the Cph1 family can be found in TCDB.

The two lysis genes cph1 and cpl1 of the Streptococcus pneumoniae bacteriophage Cp-1 coding for holin and lysozyme, respectively, have been cloned and expressed in Escherichia coli.[2] Synthesis of the Cph1 holin resulted in bacterial cell death but not lysis. The cph1 gene was able to complement a lambda SAM mutation in the non-suppressing E. coli HB101 strain to produce phage progeny, suggesting that the holins encoded by both phage genes have analogous functions and that the pneumococcal holin induces a nonspecific lesion in the cytoplasmic membrane. Concomitant expression of both holin and lysin of Cp-1 in E. coli resulted in cell lysis, apparently due to the ability of the Cpl1 lysozyme to hydrolyze the bacterium's peptidoglycan layer. The functional analysis of the cph1 and cpl1 genes cloned in a pneumococcal mutant with a complete deletion of the lytA gene, which codes for the S. pneumoniae main autolysin, provided the first direct evidence that in this gram-positive-bacterium, the Cpl1 endolysin is released to its murein substrate through the activity of the Cph1 holin. Demonstration of holin function was achieved by proving the release of pneumolysin to the periplasmic fraction, which strongly suggested that the holin produces a lesion in the pneumococcal membrane.[2][3]

See also

References

  1. ↑ Reddy, Bhaskara L.; Saier Jr., Milton H. (2013-11-01). "Topological and phylogenetic analyses of bacterial holin families and superfamilies". Biochimica et Biophysica Acta (BBA) - Biomembranes 1828 (11): 2654–2671. doi:10.1016/j.bbamem.2013.07.004. PMC 3788059. PMID 23856191.
  2. 1 2 Martín, A. C.; López, R.; García, P. (1998-01-01). "Functional analysis of the two-gene lysis system of the pneumococcal phage Cp-1 in homologous and heterologous host cells". Journal of Bacteriology 180 (2): 210–217. ISSN 0021-9193. PMC 106873. PMID 9440507.
  3. ↑ "1.E.16 The Cph1 Holin (Cph1 Holin) Family". Transporter Classification Database. Retrieved 2016-03-20.

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