Cyanothece

Cyanothece
Scientific classification
Kingdom: Bacteria
Subkingdom: Eubacteria
Phylum: Cyanobacteria
Order: Oscillatoriales
Family: Cyanothecaceae
Komárek et al. 2014[1]
Genus: Cyanothece
Komárek 1976

Cyanothece is a unicellular, diazotrophic, oxygenic photosynthesizing cyanobacteria.

Modern organisms and cellular organization

In 1976, Jiří Komárek defined the prokaryotic cyanobacteria genus Cyanothece as distinct from Synechococcus NAG 1949.[2] Organisms in both genera share characteristics in addition to being oxygenic phototrophs. They are both unicellular; forming aggregates but not found in mucilaginous colonies.[2][3] They may have a thin mucilage layer around each cell.[2] Both genera also divide by binary fission along an axis perpendicular to the cell’s longitudinal axis.[2][3][4]

A handful of characteristics distinguish the two genera. While Synechococcus are usually cylindrical, Cyanothece are normally oval and longer than 3 μm.,[2][5][6][7] Cyanothece’s outer cell wall layer is relatively thick and contains spherical, glassy vesicles whose function has yet to be defined.[2] Cyanothece’s nucleoids are spread loosely throughout the cell, with a net-like appearance.[2][3] Instead of concentric thylakoid membranes that share a center or axis, Cyanothece’s exhibit short, wavy and radially arranged.,[3][7] All Cyanothece had nitrogenase activity at one time; although some strains have lost the necessary genes.[5] During nitrogen fixing conditions, Cyanothece create inclusion storage bodies under the control of a circadian rhythm.[7]

Evolutionary history

Between 2.5 and 3 billion years ago, cyanobacteria started using the energy from light to split water, releasing oxygen into the anaerobic, reducing environment.[5][8] Parts of this ancient cyanobacterial metabolism are still maintained today.[8] Bandyopadhyay et al. 2011 created a phylogenic tree for cyanobacteria, using 226 homolog protein groups. They grouped five of the six major Cyanothece strains (PCC 7424, PCC 7822, ATCC 51142, PCC 8801, PCC 8802) as belonging to a single clade, but had Cyanothece sp PCC 7425 branched off earlier. Interestingly, PCC 7425's nitrogenase cluster is arranged differently than the other five strains and can only fix nitrogen anaerobically.[5] It has been proposed that most other cyanobacteria have lost their ability to fix nitrogen. As Earth's climate became more oxidated, the process of fixing nitrogen became unfavorable, and natural selection eliminated some of the necessary genes for the nitrogenase protein complex to increase evolutionary fitness.[5][6]

Photosynthesis/pigments

Cyanobacteria turn energy from the sun into chemical energy through oxygenic photosynthesis. Their light harvesting complex that captures the photons usually includes the pigments chlorophyll a and phycocyanin. A cyanobacterium’s typical blue-green color is a result of the combination of these two pigments. Three Cyanothece strains, sp. PCC 7424, 7822 and 8801, have the additional pigment phycoerythrin, which expands the wavelengths of light these species use for energy. Phycoerythrin also gives these three species a brownish green color.[5][9]

The rate of oxygen created by photosystem II is much higher when Cyanothece does not fix nitrogen (when the medium is nitrogen-replete).[10] The genera’s circadian rhythm controls photosynthetic oxygen generation by regulating when the proteins for their photosynthetic machinery are produced.[11][12] This diurnal oscillation occurs even when the organisms are kept in the light continuously[13][14] or in the dark continuously.[14] Photosynthesis is downregulated when the nitrogen-fixing enzyme, nitrogenase, is upregulated. Decreasing the oxygen in the cell allows the oxygen-sensitive nitrogenase to fix nitrogen from the air for the organism’s needs.[5][14]

Metabolism, biosynthesis, symbiosis

Cyanothece balances the production of oxygen through photosynthesis and oxygen-sensitive nitrogen fixation and fermentation all in one cell. They accomplish this by separating the two processes in time under the control of their circadian rhythm.[5][13] During the day, they use the energy harnessed from photosynthesis to produce the carbohydrate glycogen, which is stored in granules.[5][13] At night, the organisms break down the glycogen, providing the energy for nitrogen fixation.[13] In a very energy intensive process, nitrogenase is first synthesized[13][14] and then takes N2 from the air, combining it with protons and electrons to produce ammonia and hydrogen gas. The organisms also store cyanophycin, a nitrogen reserve molecule which is a polymer of arginine and asparagine, for use by the organism during the day.[5] Different Cyanothece species metabolize nitrogen-containing compounds through a variety of pathways; all having an arginine decarboxylase but varying after that point.[5]

To provide the anoxic environment needed by nitrogenase, Cyanothece boosts its respiration as night begins by using its glycogen stores[12] while turning off photosynthesis.[8][13] In addition, the organisms produce peroxidases and catalases which help scavenge any oxygen left in the cell.[5] The circadian rhythm ensures that this occurs even when the organism is growing in continuous light[7][13][14] or continuous darkness.[9][14] In the dark, the cyanobacterium acts as a heterotroph, getting the energy and carbon it needs from its medium. Cyanothece have the genes for the utilization of a variety of sugar molecules;[5] although, glycerol is the only one that has been used successfully to grow Cyanothece in the dark.[7][9][10][14] Many of the genes that are unique to the genera have homologs in anaerobic bacteria, including those responsible for formate production through mixed-acid fermentation and also fermentative lactate production.[5] Some Cyanothece also are capable of tryptophan degradation, methionine salvage, conversion of stored lipids into carbohydrates, alkane and higher alcohol synthesis and phosphonate metabolism.[5] They can impressively switch between a photoautotrophic and photoheterotrophic metabolism depending on the environmental conditions that maximize their growth, employing the pathways that uses the least amount of energy.[10]

Genome size, organization, and ploidy options

The genomes of many of Cyanothece have been sequenced, ranging from 4.79 to 7.84 Mbp. There are between 4367 and 6642 coding sequences that are an amalgamation of genes encoding capabilities for fermentation and aerobic nitrogen fixation (like filamentous cyanobacteria).[5] Unusually, the genes for nitrogen fixation are in a large, contiguous cluster (under the control of multiple promoters),[15] including genes for the uptake hydrogenase, regulators and transporters.[5] The organism’s robust circadian rhythm is apparent in the coordination of transcription of correlated processes.[5] Using microarrays, about 30% of 5000 genes tested exhibited diurnal oscillations in 12-hour light/dark conditions while 10% continued the behavior in continuous light.[8] 1,705 of the gene groups are >99.5% homologous with other cyanobacteria genera, largely Microcystis and filamentous nitrogen fixing strains. Typically GC content is about 40%.[5][7] Cyanothece also have 3 to 6 plasmids ranging between 10 and 330 kb.[5] Unique to some species of these genera are 1-3 linear pieces of DNA.[5][15] The linear DNA encodes enzymes for glucose and pyruvate metabolism[15] (recall that glycerol is the only organic carbon source Cyanothece has grown on successfully[10]), lactate fermentation,[8] transposons and CRISPR proteins.[5] Cyanothece do not typically use homologous recombination, which greatly hinders genetic manipulation; an exception being Cyanothece sp. PCC 7822 in which gene knock-outs can be generated.[9]

Cell size, growth patterns, sex

Cyanothece are normally oval and longer than 3 μm.[2][5][6][7] They double in 10 to 14 hours in the presence of nitrate, when they do not need to use energy to fix nitrogen, and 16 to 20 hours when fixing nitrogen.[7] They divide by binary fission in one plane that is perpendicular to their longitudinal axis.[2][6] Daughter cells remain joined for just a short time after division.[2] Cell division will not proceed until the daughter cells reach their mature size and original shape.[2][3]

Habitat range

Cyanothece have been found in a variety of environments all over the world. One point in common is that the pH is usually lower than 7.[2] Typically they are associated with water in benthic marine environments,[5] rice fields,[5] acidic marshes,[4] peaty bogs,[2] intertidal zones,[4][7] moors[3] and clear lakes,[3] but sometimes are found in mountain soils.[3]

Walls and resting cysts

Cyanothece have a thin mucilage layer around a thick outer wall that contains spherical, glassy vesicles of unknown function.[2][3] They have been shown to secrete abundant extracellular polysaccharide (EPS).[16] The EPS has been used to sequester metals from industrial waste, with more than 90% of Ni2+, Cu2+, and Co2+ removed.[16]

Storage products

As detailed in the section on metabolism, Cyanothece store the products of carbon fixation as glycogen granules which they use as an energy source during the “night”.[5][8][9][12] These glycogen granules form between the thylakoid membranes.[7] The granules are rapidly consumed to boost respiration and therefore remove the oxygen from the cell at the onset of nitrogen fixation.[8] The carboxysomes contain the carbon-fixing enzyme Rubisco and a carbon concentrating system to boost the enzyme’s efficiency. The nitrogen product, cyanophycin, is stored as a granule during nitrogen fixation and is metabolized during the “day”.[5][8][14]

Motility

Cyanothece are not flagellated. A twitching motility protein for sp. PCC 8802 is annotated on the protein database, UniProtKB <http://www.uniprot.org>.

Single-celled vs. multi-celled

Cyanothece are unicellular.[2][5][6] They can be found as free aggregates[2] but have never been found as a chain.[6]

Hydrogen production

Biohydrogen is being investigated as a clean and renewable energy source. Two main enzymes produce hydrogen in microbes, hydrogenase and nitrogenase; Cyanothece have both enzymes.[17] The nitrogenase fixes nitrogen, releasing hydrogen as a by-product. There are two different hydrogenase enzymes, an uptake hydrogenase associated with the nitrogenase and a bidirectional hydrogenase. When cultures are entrained in light-dark cycles, the nitrogenase and uptake hydrogenase are both active during the “night”, with many copies per cell.[8] Western blots show there are only a few copies of the bidirectional hydrogenase at any time.[8] 300 μmol H2/(mg Chl h) was produced by sp. ATCC 51142 from cultures that were grown in continuous light at 30 μmol photons/(m2s), anaerobic conditions, 50 mM glycerol, and without any nitrate (so the nitrogenase was active).[17] Glycerol in the medium reduces the need for carbon fixation, leaving more energy for nitrogen fixation and therefore hydrogen production.[10] It was shown by acetylene reduction that hydrogen generation and nitrogen fixation were directly proportional in this study.[17] A parallel study has demonstrated concomitant and uninterrupted production of both H2 and O2 in continuously-illuminated photobioreactor cultures, upon nitrogen-deprivation of ammonium-limited chemostat growth.[18] Further work on improving the supply of protons and electrons to nitrogenase as well as protecting it from oxygen could stimulate even better rates.

See also

References

  1. ↑ Komárek J, Kaštovský J, Mareš J, Johansen JR. (2014). "Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach" (PDF). Preslia 86: 295–335.
  2. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Komarek, J.; Cepak, V. (1998). "Cytomorphological characters supporting the taxonomic validity ofCyanothece (Cyanoprokaryota)". Plant Systematics and Evolution 210 (1-2): 25–39. doi:10.1007/BF00984725.
  3. 1 2 3 4 5 6 7 8 9 Porta, D.; Rippka, R.; Hernandez-Marine, M. (2000). "Unusual ultrastructural features in three strains of Cyanothece (cyanobacteria)". Archives of microbiology 173: 154–163. doi:10.1007/s002039900126.
  4. 1 2 3 Komárek, Jiří; Cepák, Vladislav; Kaštovský, Jan; Sulek, Josef (1 August 2004). "What are the cyanobacterial genera Cyanothece and Cyanobacterium? Contribution to the combined molecular and phenotype taxonomic evaluation of cyanobacterial diversity". Algological Studies 113 (1): 1–36. doi:10.1127/1864-1318/2004/0113-0001.
  5. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Bandyopadhyay, A.; Elvitigala, T.; Welsh, E.; Stockel, J.; Liberton, M.; Min, H.; Sherman, L. A.; Pakrasi, H. B. (4 October 2011). "Novel Metabolic Attributes of the Genus Cyanothece, Comprising a Group of Unicellular Nitrogen-Fixing Cyanobacteria". mBio 2 (5): e00214–11–e00214–11. doi:10.1128/mBio.00214-11.
  6. 1 2 3 4 5 6 Turner, S.; Huang, T.-C.; Chaw, S.-M. (2001). "Molecular phylogeny of nitrogen-fixing unicellular cyanobacteria". Botanical Bulletin of Academia Sinica 42.
  7. 1 2 3 4 5 6 7 8 9 10 Reddy, K.J.; Haskell, J.B.; Sherman, D.M.; Sherman, L.A. (1993). "Unicellular, aerobic nitrogen-fixing cyanobacteria of the genus Cyanothece". Journal of Bacteriology 175: 1284–1292.
  8. 1 2 3 4 5 6 7 8 9 10 Sherman, L.A.; Min, H.; Toepel, J.; Pakrasi, H.B. (2010). "Better living through cyanothece - unicellular diazotrophic cyanobacteria with highly versatile metabolic systems". Advances in experimental medicine and biology 675: 275–290.
  9. 1 2 3 4 5 Aryal, U. K.; Callister, S. J.; Mishra, S.; Zhang, X.; Shutthanandan, J. I.; Angel, T. E.; Shukla, A. K.; Monroe, M. E.; Moore, R. J.; Koppenaal, D. W.; Smith, R. D.; Sherman, L. (30 November 2012). "Proteome Analyses of Strains ATCC 51142 and PCC 7822 of the Diazotrophic Cyanobacterium Cyanothece sp. under Culture Conditions Resulting in Enhanced H2 Production". Applied and Environmental Microbiology 79 (4): 1070–1077. doi:10.1128/AEM.02864-12.
  10. 1 2 3 4 5 Feng, X.; Bandyopadhyay, A.; Berla, B.; Page, L.; Wu, B.; Pakrasi, H. B.; Tang, Y. J. (29 April 2010). "Mixotrophic and photoheterotrophic metabolism in Cyanothece sp. ATCC 51142 under continuous light". Microbiology 156 (8): 2566–2574. doi:10.1099/mic.0.038232-0.
  11. ↑ Basu, Subhayu; Gerchman, Yoram; Collins, Cynthia H.; Arnold, Frances H.; Weiss, Ron (28 April 2005). "A synthetic multicellular system for programmed pattern formation". Nature 434 (7037): 1130–1134. doi:10.1038/nature03461.
  12. 1 2 3 Schneegurt, M.A.; Sherman, D.M.; Nayar, S.; Sherman, L.A. (1994). "Oscillating behavior of carbohydrate granule formation and dinitrogen fixation in the cyanobacterium Cyanothece sp. strain ATCC 51142". Journal of Bacteriology 176: 1586–1597.
  13. 1 2 3 4 5 6 7 Colon-Lopez, M.S.; Sherman, D.M.; Sherman, L.A. (1997). "Transcriptional and translational regulation of nitrogenase in light-dark- and continuous-light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142". Journal of Bacteriology 179: 4319–4327.
  14. 1 2 3 4 5 6 7 8 Schneegurt, Mark A.; Tucker, Don L.; Ondr, Jennifer K.; Sherman, Debra M.; Sherman, Louis A. (9 February 2000). "Metabolic rhythms of a diazotrophic cyanobacterium, cyanothece sp. strain atcc 51142, heterotrophically grown in continuous dark". Journal of Phycology 36 (1): 107–117. doi:10.1046/j.1529-8817.2000.99152.x.
  15. 1 2 3 Welsh, E. A.; Liberton, M.; Stockel, J.; Loh, T.; Elvitigala, T.; Wang, C.; Wollam, A.; Fulton, R. S.; Clifton, S. W.; Jacobs, J. M.; Aurora, R.; Ghosh, B. K.; Sherman, L. A.; Smith, R. D.; Wilson, R. K.; Pakrasi, H. B. (23 September 2008). "The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle". Proceedings of the National Academy of Sciences 105 (39): 15094–15099. doi:10.1073/pnas.0805418105.
  16. 1 2 Shah, V.; Ray, A.; Garg, N.; Madamwar, D. (2000). "Characterization of the extracellular polysaccharide produced by a marine cyanobacterium, Cyanothece sp. ATCC 51142, and its exploitation toward metal removal from solutions.". Current microbiology 40: 274–278. doi:10.1007/s002849910054.
  17. 1 2 3 Min, H.; Sherman, L. A. (7 May 2010). "Hydrogen Production by the Unicellular, Diazotrophic Cyanobacterium Cyanothece sp. Strain ATCC 51142 under Conditions of Continuous Light". Applied and Environmental Microbiology 76 (13): 4293–4301. doi:10.1128/AEM.00146-10.
  18. ↑ Melnicki, M. R.; Pinchuk, G. E.; Hill, E. A.; Kucek, L. A.; Fredrickson, J. K.; Konopka, A.; Beliaev, A. S. (2012). "Sustained H2 Production Driven by Photosynthetic Water Splitting in a Unicellular Cyanobacterium". mBio 3 (4): e00197–12–e00197–12. doi:10.1128/mBio.00197-12. ISSN 2150-7511.
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