DDOST

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Identifiers
Symbols	DDOST ; AGER1; CDG1R; OKSWcl45; OST; OST48; WBP1
External IDs	OMIM: 602202 MGI: 1194508 HomoloGene: 3821 ChEMBL: 4239 GeneCards: DDOST Gene
EC number	2.4.99.18
Gene ontology Molecular function • oligosaccharyl transferase activity • dolichyl-diphosphooligosaccharide-protein glycotransferase activity • protein binding Cellular component • endoplasmic reticulum • endoplasmic reticulum membrane • oligosaccharyltransferase complex • membrane • integral component of membrane • intracellular membrane-bounded organelle • protein complex Biological process • translation • protein glycosylation • protein N-linked glycosylation • SRP-dependent cotranslational protein targeting to membrane • gene expression • protein N-linked glycosylation via asparagine • response to cytokine • T cell activation • post-translational protein modification • cellular protein metabolic process • innate immune response Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1650	13200
Ensembl	ENSG00000244038	ENSMUSG00000028757
UniProt	P39656	O54734
RefSeq (mRNA)	NM_005216	NM_007838
RefSeq (protein)	NP_005207	NP_031864
Location (UCSC)	Chr 1: 20.65 – 20.66 Mb	Chr 4: 138.3 – 138.31 Mb
PubMed search

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit is an enzyme that in humans is encoded by the DDOST gene.^[1][2]

This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.^[2]

References

1. Yamagata T, Tsuru T, Momoi MY, Suwa K, Nozaki Y, Mukasa T, Ohashi H, Fukushima Y, Momoi T (Jan 1998). "Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)". Genomics 45 (3): 535–40. doi:10.1006/geno.1997.4966. PMID 9367678.
2. "Entrez Gene: DDOST dolichyl-diphosphooligosaccharide-protein glycosyltransferase".

Further reading

• Kumar V, Korza G, Heinemann FS, Ozols J (1995). "Human oligosaccharyltransferase: isolation, characterization, and the complete amino acid sequence of 50-kDa subunit". Arch. Biochem. Biophys. 320 (2): 217–23. doi:10.1016/0003-9861(95)90003-9. PMID 7625827.
• Nagase T, Miyajima N, Tanaka A; et al. (1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527.
• Stitt AW, He C, Vlassara H (1999). "Characterization of the advanced glycation end-product receptor complex in human vascular endothelial cells". Biochem. Biophys. Res. Commun. 256 (3): 549–56. doi:10.1006/bbrc.1999.0291. PMID 10080935.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Lu C, He JC, Cai W; et al. (2004). "Advanced glycation endproduct (AGE) receptor 1 is a negative regulator of the inflammatory response to AGE in mesangial cells". Proc. Natl. Acad. Sci. U.S.A. 101 (32): 11767–72. doi:10.1073/pnas.0401588101. PMC 511050. PMID 15289604.
• Suzuki Y, Yamashita R, Shirota M; et al. (2004). "Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Shibatani T, David LL, McCormack AL; et al. (2005). "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits". Biochemistry 44 (16): 5982–92. doi:10.1021/bi047328f. PMID 15835887.
• Ewing RM, Chu P, Elisma F; et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.