DNA2L

DNA replication helicase/nuclease 2
Identifiers
Symbols	DNA2 ; DNA2L; hDNA2
External IDs	OMIM: 601810 MGI: 2443732 HomoloGene: 6124 GeneCards: DNA2 Gene
EC number	3.6.4.12
Gene ontology Molecular function • DNA binding • DNA helicase activity • helicase activity • nuclease activity • protein binding • ATP binding • ATPase activity • site-specific endodeoxyribonuclease activity, specific for altered base • 5'-flap endonuclease activity • 5'-3' DNA helicase activity • single-stranded DNA-dependent ATPase activity • metal ion binding • 4 iron, 4 sulfur cluster binding Cellular component • nucleus • nucleoplasm • gamma DNA polymerase complex • mitochondrial nucleoid Biological process • DNA replication checkpoint • mitotic cell cycle • telomere maintenance via recombination • telomere maintenance • DNA double-strand break processing • DNA replication • mitochondrial DNA replication • DNA strand elongation involved in DNA replication • base-excision repair • telomere maintenance via semi-conservative replication • DNA duplex unwinding • DNA replication, Okazaki fragment processing • DNA replication, removal of RNA primer • mitochondrial DNA repair • positive regulation of DNA replication • nucleic acid phosphodiester bond hydrolysis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1763	327762
Ensembl	ENSG00000138346	ENSMUSG00000036875
UniProt	P51530	Q6ZQJ5
RefSeq (mRNA)	NM_001080449	NM_177372
RefSeq (protein)	NP_001073918	NP_796346
Location (UCSC)	Chr 10: 68.41 – 68.47 Mb	Chr 10: 62.95 – 62.97 Mb
PubMed search

DNA2-like helicase is an enzyme that in humans is encoded by the DNA2 gene.^[1][2][3] Dna2, a homolog of DNA2KL present in budding yeast, possesses both helicase and nuclease activity, with which it helps catalyze early steps in homologous recombination.^[4]

References

1. Eki T, Okumura K, Shiratori A, Abe M, Nogami M, Taguchi H, Shibata T, Murakami Y, Hanaoka F (Mar 1997). "Assignment of the closest human homologue (DNA2L:KIAA0083) of the yeast Dna2 helicase gene to chromosome band 10q21.3-q22.1". Genomics 37 (3): 408–10. doi:10.1006/geno.1996.0581. PMID 8938459.
2. Masuda-Sasa T, Polaczek P, Campbell JL (Dec 2006). "Single strand annealing and ATP-independent strand exchange activities of yeast and human DNA2: possible role in Okazaki fragment maturation". J Biol Chem 281 (50): 38555–64. doi:10.1074/jbc.M604925200. PMID 17032657.
3. "Entrez Gene: DNA2L DNA2 DNA replication helicase 2-like (yeast)".
4. Mimitou, EP; Symington, LS (May 2009). "Nucleases and helicases take center stage in homologous recombination". Trends in Biochemical Science 34 (5): 264–272. doi:10.1016/j.tibs.2009.01.010. PMID 19375328.

Further reading

• Masuda-Sasa T, Imamura O, Campbell JL (2006). "Biochemical analysis of human Dna2". Nucleic Acids Res. 34 (6): 1865–75. doi:10.1093/nar/gkl070. PMC 1428797. PMID 16595800.
• Kim JH, Kim HD, Ryu GH; et al. (2006). "Isolation of human Dna2 endonuclease and characterization of its enzymatic properties". Nucleic Acids Res. 34 (6): 1854–64. doi:10.1093/nar/gkl102. PMC 1428795. PMID 16595799.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Deloukas P, Earthrowl ME, Grafham DV; et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature 429 (6990): 375–81. doi:10.1038/nature02462. PMID 15164054.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Nagase T, Miyajima N, Tanaka A; et al. (1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527.