DNA ligase (NAD+)

DNA ligase (NAD+)
Identifiers
EC number 6.5.1.2
CAS number 37259-52-2
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

DNA ligase (NAD+) (EC 6.5.1.2, polydeoxyribonucleotide synthase (NAD+), polynucleotide ligase (NAD+), DNA repair enzyme, DNA joinase, polynucleotide synthetase (nicotinamide adenine dinucleotide), deoxyribonucleic-joining enzyme, deoxyribonucleic ligase, deoxyribonucleic repair enzyme, deoxyribonucleic joinase, DNA ligase, deoxyribonucleate ligase, polynucleotide ligase, deoxyribonucleic acid ligase, polynucleotide synthetase, deoxyribonucleic acid joinase, DNA-joining enzyme, polynucleotide ligase (nicotinamide adenine dinucleotide)) is an enzyme with systematic name poly(deoxyribonucleotide):poly(deoxyribonucleotide) ligase (AMP-forming, NMN-forming).[1] This enzyme catalyses the following chemical reaction

NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m \rightleftharpoons AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m

Catalyses the formation of a phosphodiester at the site of a single-strand break in duplex DNA.

References

  1. ↑ Zimmerman, S.B., Little, J.W., Oshinsky, C.K. and Gellert, M. (1967). "Enzymatic joining of DNA strands: a novel reaction of diphosphopyridine nucleotide". Proc. Natl. Acad. Sci. USA 57: 1841–1848. doi:10.1073/pnas.57.6.1841. PMID 4291949.

See also

External links

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