Dicentric chromosome

A dicentric chromosome is an abnormal chromosome with two centromeres. It is formed through the fusion of two chromosome segments, each with a centromere, resulting in the loss of acentric fragments (lacking a centromere) and the formation of dicentric fragments.[1] The formation of dicentric chromosomes has been attributed to genetic processes, such as Robertsonian translocation[1] and paracentric inversion.[2] Dicentric chromosomes have important roles in the mitotic stability of chromosomes and the formation of pseudodicentric chromosomes.[1][3] Their existence has been linked to certain natural phenomena such as irradiation and have been documented to underlie certain clinical syndromes, notably Kabuki syndrome.[3][4] The formation of dicentric chromosomes and their implications on centromere function are studied in certain clinical cytogenetics laboratories.[5]

Formation

After a paracentric inversion, separation of the inverted chromosomes in anaphase I result in the formation of dicentric and acentric fragments. The dicentric fragments become broken, deleted products. The acentric fragments are simply lost.

Most dicentric chromosomes are known to form through chromosomal inversions, which are rotations in regions of a chromosome due to chromosomal breakages or intra-chromosomal recombinations.[2] Inversions that exclude the centromere are known as paracentric inversions, which result in unbalanced gametes after meiosis.[2] During prophase of meiosis I, homologous chromosomes form an inversion loop and crossover occurs. If a paracentric inversion has occurred, one of the products will be acentric, while the other product will be dicentric.[1][2] The dicentric chromatid is pulled apart during anaphase of meiosis I with such force that the chromosome breaks at random positions.[2] These broken fragments result in deletions of genes that lead to genetically unbalanced gametes. This can have severe consequences, contributing to the development of genetic disorders such as Kabuki syndrome and Edwards syndrome.[2]

Irradiation

Radiation is known to induce abnormalities in the nuclei of cells.[5] Dicentric chromosomes were first detected in lymphocytes from blood smears of civil and military personnel who were assigned to deal with the aftermath of the 1986 Chernobyl nuclear disaster (liquidators).[4] Radiation increases the probability that dicentric chromosomes form after every mitotic event, creating physical bridges between them in anaphase and telophase.[5] As these chromosomes are pulled apart, the chromosome bridges break, resulting in the formation of "tailed" nuclei, protrusions of the nuclei into the cytoplasm.[4]

Telomeres

When telomeres of chromosomes shorten with continued cell divisions, the chromosome ends may also fuse, forming dicentric chromosomes.[6] This is considered "crisis", a type of cell cycle arrest, and most cells in this state suffer apoptosis.[6] The resulting dicentric chromosomes are highly unstable, giving rise to chromosomal translocations, deletions and amplications, such as the Robertsonian translocation. This results in broken, deleted gene products of dicentric fragments.[1]

Applications

Dicentric chromosomes are studied in model organisms such as yeast (Saccharomyces cerevisiae).[7] The use of S. cerevisiae as a classical genetic system dates back to the 1950's[7] due to its feasibility in transformation by recombinant DNA.[7] Broken chromosomes that lead to acentric and dicentric fragments can be studied in S. cerevisiae due to its known ability to tolerate aneuploidy, an abnormal number of chromosomes.[8] Strains of S. cerevisiae that tolerate aneuploidy can stabilize products of broken chromosomes during proliferation, which can be recovered and studied in a laboratory setting.[7][8]

Cytogenetics

Centromere function has been the focus of many laboratory analyses, involving techniques like fluorescence in situ hybridization (FISH) and chromosomal banding (C-banding).[9] FISH involves the use of fluorescent probes to detect and localize specific DNA sequences on chromosomes, and centromere-specific probes can be used to measure dicentric chromosome frequencies.[10] This technique allows for the testing of human blood samples and tissue samples, which have been historically adopted by radiation laboratories.[4][5][10] C-banding is a staining technique that can be used to visualize dicentric chromosomes. It specifically stains constitutive heterochromatin, which are regions of the chromosome at or near the centromere.[9]

Consequences

The presence of "tailed" nuclei (B) in irradiated cells are considered biomarkers of dicentric chromosome formation.

The existence of dicentric chromosomes has clinically relevant consequences for individuals, which may live with intellectual, neurological and physical disabilities. Tailed nuclei are signatures of radiation exposure in human tissue, microbiota, and aquatic invertebrates from the wake of recent nuclear disasters.[5] Additionally, they are used as potential biomarkers to screen for certain genetic syndromes present in human populations.[3]

Pseudodicentric chromosomes

Dicentric chromosomes may lead to pseudodicentric mutations, in which one of the centromeres on the chromosome becomes inactivated.[1] This can have severe clinical consequences for patients, as observed in severe progressive neurological and intellectual disorders[11] such as Kabuki syndrome, a pediatric congenital disorder.[3] Kabuki syndrome is one of the most widely studied syndromes that involve the pseudodicentric chromosome.[3] Other characterized syndromes have also been linked to pseudodicentric chromosomes, such as Edwards syndrome,[12] a trisomy of chromosome 18, and Turner syndrome,[13] a loss (or partial loss) of the X chromosome. Pseudodicentric chromosomes alone do not define these syndromes, because the contribution of other chromosomal abnormalities are also considered, such as the presence of isochromosomes in Turner patients.[13]

See also

References

  1. 1 2 3 4 5 6 Nussbaum, Robert; McInnes, Roderick; Willard, Huntington; Hamosh, Ada (2007). Thompson & Thompson Genetics in Medicine. Philadelphia(PA): Saunders. p. 72. ISBN 978-1-4160-3080-5.
  2. 1 2 3 4 5 6 Hartwell, Leland; Hood, Leeroy; Goldberg, Michael; Reynolds, Ann; Lee, Silver (2011). Genetics From Genes to Genomes, 4e. New York: McGraw-Hill. ISBN 9780073525266.
  3. 1 2 3 4 5 Lynch, Sally; et al. (1995). "Kabuki syndrome-like features in monozygotic twin boys with a pseudodicentric chromosome 13" (PDF). J. Med. Genet. (32:227-230).
  4. 1 2 3 4 Kravtsov, V. Yu; Fedortseva, R. F; Starkova, Ye. V; Yartseva, N. M; Nikiforov, A. M (2000-05-15). "Tailed nuclei and dicentric chromosomes in irradiated subjects". Applied Radiation and Isotopes 52 (5): 1121–1127. doi:10.1016/S0969-8043(00)00057-9.
  5. 1 2 3 4 5 Anbumani, S.; Mohankumar, Mary N. (2015-08-12). "Nucleoplasmic bridges and tailed nuclei are signatures of radiation exposure in Oreochromis mossambicus using erythrocyte micronucleus cytome assay (EMNCA)". Environmental Science and Pollution Research: 1–12. doi:10.1007/s11356-015-5107-1. ISSN 0944-1344.
  6. 1 2 Boukamp, Petra; Popp, Susanne; Krunic, Damir (2005-11-01). "Telomere-Dependent Chromosomal Instability". Journal of Investigative Dermatology Symposium Proceedings 10 (2): 89–94. doi:10.1111/j.1087-0024.2005.200401.x. ISSN 1087-0024.
  7. 1 2 3 4 Haber, James; Thorburn, Patricia; Rogers, David (1983). "Meiotic and mitotic behavior of dicentric chromosomes in Saccharomyces cerevisiae". Genetics (106:185-205).
  8. 1 2 Torres, Eduardo M.; Dephoure, Noah; Panneerselvam, Amudha; Tucker, Cheryl M.; Whittaker, Charles A.; Gygi, Steven P.; Dunham, Maitreya J.; Amon, Angelika (2010-01-10). "Identification of Aneuploidy-Tolerating Mutations". Cell 143 (1): 71–83. doi:10.1016/j.cell.2010.08.038. ISSN 0092-8674. PMC 2993244. PMID 20850176.
  9. 1 2 Lefort, Genevieve; et al. (2002). "Stable dicentric duplication‐deficiency chromosome 14 resulting from crossing‐over within a maternal paracentric inversion". Am. J. Med. Genet. (113:333-338).
  10. 1 2 Bhavani, M.; Tamizh Selvan, G.; Kaur, Harpreet; Adhikari, J. S.; Vijayalakshmi, J.; Venkatachalam, P.; Chaudhury, N. K. (2014-09-01). "Dicentric chromosome aberration analysis using giemsa and centromere specific fluorescence in-situ hybridization for biological dosimetry: An inter- and intra-laboratory comparison in Indian laboratories". Applied Radiation and Isotopes 92: 85–90. doi:10.1016/j.apradiso.2014.06.004.
  11. ↑ Rivera, H.; Zuffardi, O.; Maraschio, P.; Caiulo, A.; Anichini, C.; Scarinci, R.; Vivarelli, R. (1989-10-01). "Alternate centromere inactivation in a pseudodicentric (15;20)(pter;pter) associated with a progressive neurological disorder.". Journal of Medical Genetics 26 (10): 626–630. doi:10.1136/jmg.26.10.626. ISSN 1468-6244. PMID 2685311.
  12. ↑ Gravholt, Claus Højbjerg; Bugge, Merete; Strømkjær, Helle; Caprani, Monna; Henriques, Ulrik; Petersen, Michael B.; Brandt, Carsten A. (1997-07-01). "A patient with Edwards syndrome caused by a rare pseudodicentric chromosome 18 of paternal origin". Clinical Genetics 52 (1): 56–60. doi:10.1111/j.1399-0004.1997.tb02515.x. ISSN 1399-0004.
  13. 1 2 Monroy, Nancy; López, Marisol; Cervantes, Alicia; García-Cruz, Diana; Zafra, Gildardo; Canún, Sonia; Zenteno, Juan Carlos; Kofman-Alfaro, Susana (2002-01-22). "Microsatellite analysis in Turner syndrome: Parental origin of X chromosomes and possible mechanism of formation of abnormal chromosomes". American Journal of Medical Genetics 107 (3): 181–189. doi:10.1002/ajmg.10113. ISSN 1096-8628.
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