EHD1

EH-domain containing 1
Rendering based on PDB 2JQ6​.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2JQ6​, 2KFF​, 2KFG​, 2KFH​, 2KSP​
Identifiers
Symbols	EHD1 ; H-PAST; HPAST1; PAST; PAST1
External IDs	OMIM: 605888 MGI: 1341878 HomoloGene: 81678 GeneCards: EHD1 Gene
Gene ontology Molecular function • calcium ion binding • protein binding • ATP binding • GTP binding • Rab GTPase binding Cellular component • lipid particle • plasma membrane • endosome membrane • membrane • ciliary pocket membrane • platelet dense tubular network membrane • early endosome membrane • recycling endosome membrane • extracellular exosome Biological process • intracellular protein transport • endocytosis • blood coagulation • positive regulation of cholesterol storage • neuron projection development • endocytic recycling • low-density lipoprotein particle clearance • cilium assembly • cholesterol homeostasis • protein homooligomerization • protein localization to cilium • positive regulation of myoblast fusion • cellular response to nerve growth factor stimulus • positive regulation of endocytic recycling Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10938	13660
Ensembl	ENSG00000110047	ENSMUSG00000024772
UniProt	Q9H4M9	Q9WVK4
RefSeq (mRNA)	NM_001282444	NM_010119
RefSeq (protein)	NP_001269373	NP_034249
Location (UCSC)	Chr 11: 64.85 – 64.89 Mb	Chr 19: 6.28 – 6.3 Mb
PubMed search

EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1gene.^[1] belonging to the EHD protein family.

Function

This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors.^[1]

Interactions

EHD1 has been shown to interact with Insulin-like growth factor 1 receptor^[2] and SNAP29.^[2]

References

1. "Entrez Gene: EHD1 EH-domain containing 1".
2. Rotem-Yehudar, R; Galperin E; Horowitz M (Aug 2001). "Association of insulin-like growth factor 1 receptor with EHD1 and SNAP29". J. Biol. Chem. (United States) 276 (35): 33054–60. doi:10.1074/jbc.M009913200. ISSN 0021-9258. PMID 11423532.

Further reading

• Di Fiore PP, Pelicci PG, Sorkin A (1998). "EH: a novel protein-protein interaction domain potentially involved in intracellular sorting". Trends Biochem. Sci. 22 (11): 411–3. doi:10.1016/S0968-0004(97)01127-4. PMID 9397678.
• Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Yu W, Andersson B, Worley KC, et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Guru SC, Agarwal SK, Manickam P, et al. (1997). "A Transcript Map for the 2.8-Mb Region Containing the Multiple Endocrine Neoplasia Type 1 Locus". Genome Res. 7 (7): 725–35. doi:10.1101/gr.7.7.725. PMC 310681. PMID 9253601.
• Mintz L, Galperin E, Pasmanik-Chor M, et al. (1999). "EHD1--an EH-domain-containing protein with a specific expression pattern". Genomics 59 (1): 66–76. doi:10.1006/geno.1999.5800. PMID 10395801.
• Haider NB, Searby C, Galperin E, et al. (2000). "Evaluation and molecular characterization of EHD1, a candidate gene for Bardet-Biedl syndrome 1 (BBS1)". Gene 240 (1): 227–32. doi:10.1016/S0378-1119(99)00395-9. PMID 10564830.
• Pohl U, Smith JS, Tachibana I, et al. (2000). "EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins". Genomics 63 (2): 255–62. doi:10.1006/geno.1999.6087. PMID 10673336.
• Rotem-Yehudar R, Galperin E, Horowitz M (2001). "Association of insulin-like growth factor 1 receptor with EHD1 and SNAP29". J. Biol. Chem. 276 (35): 33054–60. doi:10.1074/jbc.M009913200. PMID 11423532.
• Caplan S, Naslavsky N, Hartnell LM, et al. (2002). "A tubular EHD1-containing compartment involved in the recycling of major histocompatibility complex class I molecules to the plasma membrane". EMBO J. 21 (11): 2557–67. doi:10.1093/emboj/21.11.2557. PMC 126039. PMID 12032069.
• Galperin E, Benjamin S, Rapaport D, et al. (2003). "EHD3: a protein that resides in recycling tubular and vesicular membrane structures and interacts with EHD1". Traffic 3 (8): 575–89. doi:10.1034/j.1600-0854.2002.30807.x. PMID 12121420.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Naslavsky N, Boehm M, Backlund PS, Caplan S (2005). "Rabenosyn-5 and EHD1 Interact and Sequentially Regulate Protein Recycling to the Plasma Membrane". Mol. Biol. Cell 15 (5): 2410–22. doi:10.1091/mbc.E03-10-0733. PMC 404033. PMID 15020713.
• Guilherme A, Soriano NA, Furcinitti PS, Czech MP (2004). "Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes". J. Biol. Chem. 279 (38): 40062–75. doi:10.1074/jbc.M401918200. PMID 15247266.
• Xu Y, Shi H, Wei S, et al. (2005). "Mutually exclusive interactions of EHD1 with GS32 and syndapin II". Mol. Membr. Biol. 21 (4): 269–77. doi:10.1080/09687680410001716871. PMID 15371016.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Lee DW, Zhao X, Scarselletta S, et al. (2005). "ATP binding regulates oligomerization and endosome association of RME-1 family proteins". J. Biol. Chem. 280 (17): 17213–20. doi:10.1074/jbc.M412751200. PMID 15710626.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.