ENPP3

Ectonucleotide pyrophosphatase/phosphodiesterase 3
Identifiers
Symbols	ENPP3 ; B10; CD203c; NPP3; PD-IBETA; PDNP3
External IDs	OMIM: 602182 MGI: 2143702 HomoloGene: 3683 ChEMBL: 5580 GeneCards: ENPP3 Gene
EC number	3.1.4.1, 3.6.1.9
Gene ontology Molecular function • nucleic acid binding • phosphodiesterase I activity • nucleotide diphosphatase activity • scavenger receptor activity • polysaccharide binding • NADH pyrophosphatase activity • metal ion binding • nucleoside-triphosphate diphosphatase activity Cellular component • integral component of plasma membrane • perinuclear region of cytoplasm • extracellular exosome Biological process • phosphate-containing compound metabolic process • receptor-mediated endocytosis • immune response • nucleoside triphosphate catabolic process • inorganic diphosphate transport • nucleic acid phosphodiester bond hydrolysis Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5169	209558
Ensembl	ENSG00000154269	ENSMUSG00000019989
UniProt	O14638	Q6DYE8
RefSeq (mRNA)	NM_005021	NM_134005
RefSeq (protein)	NP_005012	NP_598766
Location (UCSC)	Chr 6: 131.63 – 131.75 Mb	Chr 10: 24.77 – 24.84 Mb
PubMed search

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 is an enzyme that in humans is encoded by the ENPP3 gene.^[1][2]

Function

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells.^[2]

This protein has also been used in conjunction with CD63 as a marker for activated basophils in the Basophil Activation Test for IgE mediated allergic reactions.^[3]

References

1. Jin-Hua P, Goding JW, Nakamura H, Sano K (October 1997). "Molecular cloning and chromosomal localization of PD-Ibeta (PDNP3), a new member of the human phosphodiesterase I genes". Genomics 45 (2): 412–5. doi:10.1006/geno.1997.4949. PMID 9344668.
2. "Entrez Gene: ENPP3 ectonucleotide pyrophosphatase/phosphodiesterase 3".
3. McGowan EC, Saini S (February 2013). "Update on the performance and application of basophil activation tests". Current Allergy and Asthma Reports 13 (1): 101–9. doi:10.1007/s11882-012-0324-x. PMID 23188565.

Further reading

• Bühring HJ, Streble A, Valent P (April 2004). "The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis". International Archives of Allergy and Immunology 133 (4): 317–29. doi:10.1159/000077351. PMID 15031605.
• Andoh K, Piao JH, Terashima K, Nakamura H, Sano K (September 1999). "Genomic structure and promoter analysis of the ecto-phosphodiesterase I gene (PDNP3) expressed in glial cells". Biochimica et Biophysica Acta 1446 (3): 213–24. doi:10.1016/s0167-4781(99)00090-1. PMID 10524196.
• Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K (May 2001). "The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3". Blood 97 (10): 3303–5. doi:10.1182/blood.V97.10.3303. PMID 11342463.
• Yano Y, Hayashi Y, Sano K, Shinmaru H, Kuroda Y, Yokozaki H, Yoon S, Kasuga M (November 2003). "Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-3 (E-NPP3/CD203c/PD-I beta/B10/gp130RB13-6) in human colon carcinoma". International Journal of Molecular Medicine 12 (5): 763–6. doi:10.3892/ijmm.12.5.763. PMID 14533006.
• Yano Y, Hayashi Y, Sano K, Nagano H, Nakaji M, Seo Y, Ninomiya T, Yoon S, Yokozaki H, Kasuga M (April 2004). "Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-1 (E-NPP1/PC-1) and -3 (E-NPP3/CD203c/PD-Ibeta/B10/gp130(RB13-6)) in inflammatory and neoplastic bile duct diseases". Cancer Letters 207 (2): 139–47. doi:10.1016/j.canlet.2003.11.002. PMID 15072822.
• Elbatarny HS, Maurice DH (October 2005). "Leptin-mediated activation of human platelets: involvement of a leptin receptor and phosphodiesterase 3A-containing cellular signaling complex". American Journal of Physiology. Endocrinology and Metabolism 289 (4): E695–702. doi:10.1152/ajpendo.00125.2005. PMID 15886225.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (January 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Ocmant A, Peignois Y, Mulier S, Hanssens L, Michils A, Schandené L (March 2007). "Flow cytometry for basophil activation markers: the measurement of CD203c up-regulation is as reliable as CD63 expression in the diagnosis of cat allergy". Journal of Immunological Methods 320 (1-2): 40–8. doi:10.1016/j.jim.2006.12.002. PMID 17275019.