Edward Marcotte

Edward Marcotte is a professor of biochemistry at The University of Texas at Austin, working in genetics, proteomics, and bioinformatics.[1] Marcotte is an example of a computational biologist who also relies on experiments to validate bioinformatics-based predictions.[2]

Education and positions

Marcotte's undergraduate education was at The University of Texas at Austin, where he received a B.S. in Microbiology in 1990. He received his Ph.D. in Biochemistry from The University of Texas at Austin in 1995, and did his postdoctoral work both at UT Austin and at University of California, Los Angeles with Professor David Eisenberg. Marcotte has been a professor at UT Austin since 2001.

Research

Marcotte's major research contributions are in the areas of bioinformatics, proteomics, systems biology, and synthetic biology.

Bioinformatics and systems biology

In early work, Marcotte and colleagues created the first genome-scale map of functional links among proteins in any complex organism (the yeast Saccharomyces cerevisiae), an approach that allowed them to predict the function to more than half of all uncharacterized yeast proteins.[3] Marcotte also developed several methods of identifying functional interactions between proteins, including phylogenetic profiling,[4][5][6] Rosetta Stone gene fusion,[7] mRNA coexpression,[3] and mirror tree[8] approaches.

In 2010, Marcotte and colleagues identified an algorithm for identifying cases of deep homology based on phenotype.[1][9]

Proteomics

In the field of proteomics, Marcotte's contributions include developing early versions of the human protein interaction network[10][11] and mapping of >7,000 human protein interactions.[12] Marcotte and colleagues developed the spotted cell microarray technique for high-throughput measurement of protein expression, subcellular location, and function,[11][13][14][15] developed algorithms for analyzing mass spectrometry data,[16][17][18][19] started an open access database for mass spectrometry proteomics data,[20] and developed the APEX method for absolute protein quantification on a proteome-wide scale.[21][22] Using APEX, Marcotte and colleagues demonstrated that protein abundance in a lower eukaryote is predominantly determined by mRNA levels, while human protein abundances are determined roughly equally by transcriptional and post-transcriptional regulation.[23]

References

  1. 1 2 Zimmer, Carl. "The Search for Genes Leads to Unexpected Places", The New York Times, New York, April 26, 2010.
  2. ↑ "UT Austin Department of Chemistry & Biochemistry". Retrieved 6 September 2012.
  3. 1 2 Marcotte, E.M.; Pellegrini, M.; Thompson, M. J.; Yeates, T.; Eisenberg, D. (1999). "A Combined Algorithm for Genome-Wide Prediction of Protein Function". Nature 402 (6757): 83–86. doi:10.1038/47048. PMID 10573421.
  4. ↑ Pellegrini, M.; Marcotte, E. M.; Thompson, M. J.; Eisenberg, D.; Yeates, T. O. (1999). "Detecting the Components of Protein Complexes and Pathways by Comparative Genome Analysis: Protein Phylogenetic Profiles". Proc. Natl. Acad. Sci. U.S.A. 96: 4285–4288. doi:10.1073/pnas.96.8.4285. PMC 16324. PMID 10200254.
  5. ↑ Marcotte, E.M.; Xenarios, I., van Der Bliek, A. M., Eisenberg, D. "Localizing proteins in the cell from their phylogenetic profiles". Proc. Natl. Acad. Sci. U.S.A. 97: 12115–20. doi:10.1073/pnas.220399497. Cite uses deprecated parameter |coauthors= (help)
  6. ↑ Date, S.V.; Marcotte, E. M. (2003). "Discovery of uncharacterized cellular systems by genome-wide analysis of functional linkages". Nature Biotechnology 21: 1055–1062. doi:10.1038/nbt861.
  7. ↑ Marcotte, E.M.; Pellegrini, M., Ng, H.-L., Rice, D. W., Yeates, T. O., Eisenberg, D. (1999). "Detecting Protein Function & Protein-Protein Interactions from Genome Sequences". Science 285 (5428): 751–753. doi:10.1126/science.285.5428.751. PMID 10427000. Cite uses deprecated parameter |coauthors= (help)
  8. ↑ Ramani, A.K.; Marcotte, E. M. (2003). "Exploiting the Co-evolution of Interacting Proteins to Discover Interaction Specificity.". J. Mol. Biol. 327: 273–284. doi:10.1016/s0022-2836(03)00114-1.
  9. ↑ McGary KL; Park TJ; Woods JO; Cha HJ; Wallingford JB; Marcotte EM (April 2010). "Systematic discovery of nonobvious human disease models through orthologous phenotypes" (PDF). Proceedings of the National Academy of Sciences 107 (14): 6544–9. doi:10.1073/pnas.0910200107. PMC 2851946. PMID 20308572.
  10. ↑ Ramani, A.K.; Bunescu, R. C.; Mooney, R. J.; Marcotte, E. M. (2005). "Consolidating the set of known human protein-protein interactions in preparation for large-scale mapping of the human interactome.". Genome Biology 6: R40.1–12. doi:10.1186/gb-2005-6-5-r40. PMID 15892868.
  11. 1 2 Narayanaswamy, R.; Niu, W.; Scouras, A.; Hart, G. T.; Davies, J.; Ellington, A. D.; Iyer, V. R.; Marcotte, E. M. (2006). "Systematic profiling of cellular phenotypes with spotted cell microarrays reveals new pheromone response genes.". Genome Biology 7: R6. doi:10.1186/gb-2006-7-1-r6. PMID 16507139.
  12. ↑ Ramani, A.K.; Li, Z.; Hart, G. T.; Carlson, M. W.; Boutz, D.; Marcotte, E. M. (2008). "A map of human protein interactions derived from co-expression of human mRNAs and their orthologs.". Mol. Sys. Biol. 4: 180. doi:10.1038/msb.2008.19.
  13. ↑ Zhao, J.; Niu, W.; Yao, J.; Mohr, S.; Marcotte, E. M.; Lambowitz, A. M. (2008). "Group II intron protein localization and insertion sites are affected by polyphosphate.". PLoS Biology 6: e150. doi:10.1371/journal.pbio.0060150.
  14. ↑ Narayanaswamy, R.; Moradi, E. K.; Niu, W.; Hart, G. T.; Davis, M.; McGary, K. L.; Ellington, A. D.; Marcotte, E. M. (2009). "Systematic definition of protein constituents along the major polarization axis reveals an adaptive reuse of the polarization machinery in pheromone-treated budding yeast.". J. Proteome Research 8: 6–19. doi:10.1021/pr800524g.
  15. ↑ Narayanaswamy, R.; Levy, M.; Tsechansky, M.; Stovall, G. M.; O’Connell, J.; Mirrielees, J.; Ellington, A. D.; Marcotte, E. M. (2009). "Widespread reorganization of metabolic enzymes into reversible assemblies upon nutrient starvation.". Proc. Natl. Acad. Sci. U.S.A. 106: 10147–52. doi:10.1073/pnas.0812771106.
  16. ↑ Ramakrishnan, S.; Mao, R.; Nakorchevskiy, A. A.; Prince, J. T.; Willard, W. S.; Xu, W.; Marcotte, E. M.; Miranker, D. P. (2006). "A fast coarse filtering method for protein identification by mass spectrometry.". Bioinformatics 22: 1524–31. doi:10.1093/bioinformatics/btl118.
  17. ↑ Prince, J.T.; Marcotte, E. M. (2008). "mspire: Mass spectrometry proteomics in Ruby.". Bioinformatics 24: 2796–7. doi:10.1093/bioinformatics/btn513. PMC 2639276. PMID 18930952.
  18. ↑ Ramakrishnan, S.R.; Vogel, C.; Prince, J. T.; Li, Z.; Penalva, L. O.; Myers, M.; Marcotte, E. M.; Miranker, D. P. (2009). "Integrating shotgun proteomics and mRNA expression data to improve protein identification.". Bioinformatics 25: 1397–1403. doi:10.1093/bioinformatics/btp168.
  19. ↑ Prince, J.T.; Marcotte, E. M. (2006). "Chromatographic alignment of ESI-LC-MS proteomics datasets by ordered bijective interpolated warping.". Analytical Chemistry 78: 6140–6152. doi:10.1021/ac0605344.
  20. ↑ Prince, J.T.; Carlson, M. W; Wang, R.; Lu, P.; Marcotte, E. M. (2004). "The need for a public proteomics repository.". Nature Biotechnology 22: 471–2. doi:10.1038/nbt0404-471.
  21. ↑ Lu, P.; Vogel, C.; Wang, R.; Yao, X.; Marcotte, E. M. (2007). "Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation.". Nature 25 (1): 117–20. doi:10.1038/nbt1270.
  22. ↑ Vogel, C.; Marcotte E. M. (2008). "Calculating absolute and relative protein abundance from mass spectrometry based protein expression data.". Nature Protocols 3: 1444–1451. doi:10.1038/nprot.2008.132.
  23. ↑ Vogel, C.; de Sousa Abreu, R., Ko, D., Le, S.-Y., Shapiro, B. A., Sandhu, D., Boutz, D., Marcotte, E. M., Penalva, L. O. (2010). "Sequence signatures and mRNA concentration can explain two-thirds of protein abundance variation in a human cell line.". Molecular Systems Biology 6: 400. doi:10.1038/msb.2010.59. Cite uses deprecated parameter |coauthors= (help)


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