Endothiapepsin

Endothiapepsin
Identifiers
EC number 3.4.23.22
CAS number 37205-60-0
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Endothiapepsin (EC 3.4.23.22, Endothia aspartic proteinase, Endothia acid proteinase, Endothia parasitica acid proteinase, Endothia parasitica aspartic proteinase) is an enzyme.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

Hydrolysis of proteins with specificity similar to that of pepsin A; prefers hydrophobic residues at P1 and P1', but does not cleave Ala14-Leu in the B chain of insulin or Z-Glu-Tyr. Clots milk

This enzyme is isolated from the ascomycete Endothia parasitica.

References

  1. ↑ Blundell, T. L.; Jenkins, J. A.; Sewell, B. T.; Pearl, L. H.; Cooper, J. B.; Tickle, I. J.; Veerapandian, B; Wood, S. P. (1990). "X-ray analyses of aspartic proteinases. The three-dimensional structure at 2.1 a resolution of endothiapepsin". Journal of Molecular Biology 211 (4): 919–41. doi:10.1016/0022-2836(90)90084-Y. PMID 2179568.
  2. ↑ Hemmings, A. M.; Foundling, S. I.; Sibanda, B. L.; Wood, S. P.; Pearl, L. H.; Blundell, T (1985). "Energy calculations on aspartic proteinases: Human renin, endothiapepsin and its complex with an angiotensinogen fragment analogue, H-142". Biochemical Society Transactions 13 (6): 1036–41. doi:10.1042/bst0131036. PMID 3912234.
  3. ↑ Whitaker, J.R. (1970). "Protease of Endothia parasitica". Methods Enzymol. 19: 436–445. doi:10.1016/0076-6879(70)19032-x.
  4. ↑ Williams, D.C., Whitaker, J.R. and Caldwell, P.V. (1972). "Hydrolysis of peptide bonds of the oxidized B-chain of insulin by Endothia parasitica protease". Arch. Biochem. Biophys. 149: 52–61. doi:10.1016/0003-9861(72)90298-6. PMID 4552802.
  5. ↑ Barkholt, V. (1987). "Amino acid sequence of endothiapepsin. Complete primary structure of the aspartic protease from Endothia parasitica". Eur. J. Biochem. 167: 327–338. doi:10.1111/j.1432-1033.1987.tb13340.x. PMID 3305016.
  6. ↑ Cooper, J., Foundling, S., Hemmings, A., Blundell, T., Jones, D.M., Hallett, A. and Szelke, M. (1987). "The structure of a synthetic pepsin inhibitor complexed with endothiapepsin". Eur. J. Biochem. 169: 215–221. doi:10.1111/j.1432-1033.1987.tb13600.x. PMID 3119339.

External links

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