FARP2

FERM, RhoGEF and pleckstrin domain protein 2
Identifiers
Symbols	FARP2 ; FIR; FRG; PLEKHC3
External IDs	MGI: 2385126 HomoloGene: 8877 GeneCards: FARP2 Gene
Gene ontology Molecular function • Rho guanyl-nucleotide exchange factor activity • cytoskeletal protein binding • Rac guanyl-nucleotide exchange factor activity Cellular component • cytoplasm • cytosol • cytoskeleton • extrinsic component of membrane Biological process • cell adhesion • axon guidance • neuron remodeling • Rac protein signal transduction • osteoclast differentiation • actin cytoskeleton reorganization • regulation of integrin activation • regulation of Rho protein signal transduction • positive regulation of GTPase activity • semaphorin-plexin signaling pathway • podosome assembly Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9855	227377
Ensembl	ENSG00000006607	ENSMUSG00000034066
UniProt	O94887	Q91VS8
RefSeq (mRNA)	NM_001282983	NM_145519
RefSeq (protein)	NP_001269912	NP_663494
Location (UCSC)	Chr 2: 241.36 – 241.49 Mb	Chr 1: 93.51 – 93.62 Mb
PubMed search

FERM, RhoGEF and pleckstrin domain-containing protein 2 is a protein that in humans is encoded by the FARP2 gene.^[1][2][3]

Model organisms

Farp2 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Micronucleus test	Normal
Heart weight	Normal
Tail epidermis wholemount	Normal
Skin Histopathology	Abnormal
Brain histopathology	Abnormal
Salmonella infection	Normal[4]
Citrobacter infection	Normal[5]
All tests and analysis from[6][7]

Model organisms have been used in the study of FARP2 function. A conditional knockout mouse line, called Farp2^{tm1a(KOMP)Wtsi[8][9]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.^[10][11][12]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[6][13] Twenty four tests were carried out on mutant mice and two significant abnormalities were observed.^[6] Homozygous mutant animals had a thickened cerebral cortex and displayed abnormal hair shedding.^[6]

Interactions

FARP2 has been shown to interact with PDZK1.^[14]

References

1. Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Oct 1998). "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research 5 (5): 277–86. doi:10.1093/dnares/5.5.277. PMID 9872452.
2. Kubo T, Yamashita T, Yamaguchi A, Sumimoto H, Hosokawa K, Tohyama M (Oct 2002). "A novel FERM domain including guanine nucleotide exchange factor is involved in Rac signaling and regulates neurite remodeling". The Journal of Neuroscience 22 (19): 8504–13. PMID 12351724.
3. "Entrez Gene: FARP2 FERM, RhoGEF and pleckstrin domain protein 2".
4. "Salmonella infection data for Farp2". Wellcome Trust Sanger Institute.
5. "Citrobacter infection data for Farp2". Wellcome Trust Sanger Institute.
6. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
7. Mouse Resources Portal, Wellcome Trust Sanger Institute.
8. "International Knockout Mouse Consortium".
9. "Mouse Genome Informatics".
10. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
11. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
12. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
13. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
14. Gisler SM, Pribanic S, Bacic D, Forrer P, Gantenbein A, Sabourin LA, Tsuji A, Zhao ZS, Manser E, Biber J, Murer H (Nov 2003). "PDZK1: I. a major scaffolder in brush borders of proximal tubular cells". Kidney International 64 (5): 1733–45. doi:10.1046/j.1523-1755.2003.00266.x. PMID 14531806.

Further reading

• Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (Aug 2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Current Biology 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
• Gisler SM, Pribanic S, Bacic D, Forrer P, Gantenbein A, Sabourin LA, Tsuji A, Zhao ZS, Manser E, Biber J, Murer H (Nov 2003). "PDZK1: I. a major scaffolder in brush borders of proximal tubular cells". Kidney International 64 (5): 1733–45. doi:10.1046/j.1523-1755.2003.00266.x. PMID 14531806.
• Miyamoto Y, Yamauchi J, Itoh H (Aug 2003). "Src kinase regulates the activation of a novel FGD-1-related Cdc42 guanine nucleotide exchange factor in the signaling pathway from the endothelin A receptor to JNK". The Journal of Biological Chemistry 278 (32): 29890–900. doi:10.1074/jbc.M301559200. PMID 12771149.
• "Toward a complete human genome sequence". Genome Research 8 (11): 1097–108. Nov 1998. doi:10.1101/gr.8.11.1097. PMID 9847074.