FBXO5

F-box protein 5
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2M6N​
Identifiers
Symbols	FBXO5 ; EMI1; FBX5; Fbxo31
External IDs	OMIM: 606013 MGI: 1914391 HomoloGene: 8135 GeneCards: FBXO5 Gene
Gene ontology Molecular function • protein binding • protein kinase binding • metal ion binding Cellular component • nucleus • nucleoplasm • cytoplasm • spindle • cytosol Biological process • G1/S transition of mitotic cell cycle • regulation of transcription involved in G1/S transition of mitotic cell cycle • mitotic cell cycle • oocyte maturation • spindle assembly involved in female meiosis I • regulation of mitotic nuclear division • metaphase/anaphase transition of mitotic cell cycle • regulation of mitotic cell cycle • vesicle organization • anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process • negative regulation of meiotic nuclear division • microtubule polymerization • cell division • negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition • regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	26271	67141
Ensembl	ENSG00000112029	ENSMUSG00000019773
UniProt	Q9UKT4	Q7TSG3
RefSeq (mRNA)	NM_001142522	NM_025995
RefSeq (protein)	NP_001135994	NP_080271
Location (UCSC)	Chr 6: 152.97 – 152.98 Mb	Chr 10: 5.8 – 5.81 Mb
PubMed search

F-box only protein 5 is a protein that in humans is encoded by the FBXO5 gene.^[1][2][3]

Function

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex.^[3]

Interactions

FBXO5 has been shown to interact with:

• CDC20,^[4]
• FZR1,^[4] and
• SKP1A.^[5]

References

1. Cenciarelli C, Chiaur DS, Guardavaccaro D, Parks W, Vidal M, Pagano M (Dec 1999). "Identification of a family of human F-box proteins". Curr Biol 9 (20): 1177–9. doi:10.1016/S0960-9822(00)80020-2. PMID 10531035.
2. Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW (Dec 1999). "A family of mammalian F-box proteins". Curr Biol 9 (20): 1180–2. doi:10.1016/S0960-9822(00)80021-4. PMID 10531037.
3. "Entrez Gene: FBXO5 F-box protein 5".
4. Hsu JY, Reimann JD, Sørensen CS, Lukas J, Jackson PK (May 2002). "E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1)". Nat. Cell Biol. 4 (5): 358–66. doi:10.1038/ncb785. PMID 11988738.
5. Cenciarelli C, Chiaur DS, Guardavaccaro D, Parks W, Vidal M, Pagano M (Oct 1999). "Identification of a family of human F-box proteins". Curr. Biol. 9 (20): 1177–9. doi:10.1016/S0960-9822(00)80020-2. PMID 10531035.

Further reading

• Gubin AN, Njoroge JM, Bouffard GG, Miller JL (1999). "Gene expression in proliferating human erythroid cells". Genomics 59 (2): 168–77. doi:10.1006/geno.1999.5855. PMID 10409428.
• Chiaur DS, Murthy S, Cenciarelli C, Parks W, Loda M, Inghirami G, Demetrick D, Pagano M (2000). "Five human genes encoding F-box proteins: chromosome mapping and analysis in human tumors". Cytogenet. Cell Genet. 88 (3–4): 255–8. doi:10.1159/000015532. PMID 10828603.
• Reimann JD, Freed E, Hsu JY, Kramer ER, Peters JM, Jackson PK (2001). "Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex". Cell 105 (5): 645–55. doi:10.1016/S0092-8674(01)00361-0. PMID 11389834.
• Reimann JD, Jackson PK (2002). "Emi1 is required for cytostatic factor arrest in vertebrate eggs". Nature 416 (6883): 850–4. doi:10.1038/416850a. PMID 11976684.
• Hsu JY, Reimann JD, Sørensen CS, Lukas J, Jackson PK (2002). "E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1)". Nat. Cell Biol. 4 (5): 358–66. doi:10.1038/ncb785. PMID 11988738.
• Li Z, Hu CY, Mo BQ, Xu JD, Zhao Y (2003). "[Effect of beta-carotene on gene expression of breast cancer cells]". Ai Zheng 22 (4): 380–4. PMID 12703993.
• Margottin-Goguet F, Hsu JY, Loktev A, Hsieh HM, Reimann JD, Jackson PK (2003). "Prophase destruction of Emi1 by the SCF(betaTrCP/Slimb) ubiquitin ligase activates the anaphase promoting complex to allow progression beyond prometaphase". Dev. Cell 4 (6): 813–26. doi:10.1016/S1534-5807(03)00153-9. PMID 12791267.
• Moshe Y, Boulaire J, Pagano M, Hershko A (2004). "Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphase promoting complex/cyclosome". Proc. Natl. Acad. Sci. U.S.A. 101 (21): 7937–42. doi:10.1073/pnas.0402442101. PMC 419535. PMID 15148369.
• Hansen DV, Loktev AV, Ban KH, Jackson PK (2005). "Plk1 Regulates Activation of the Anaphase Promoting Complex by Phosphorylating and Triggering SCFβTrCP-dependent Destruction of the APC Inhibitor Emi1". Mol. Biol. Cell 15 (12): 5623–34. doi:10.1091/mbc.E04-07-0598. PMC 532041. PMID 15469984.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Eldridge AG, Loktev AV, Hansen DV, Verschuren EW, Reimann JD, Jackson PK (2006). "The evi5 oncogene regulates cyclin accumulation by stabilizing the anaphase-promoting complex inhibitor emi1". Cell 124 (2): 367–80. doi:10.1016/j.cell.2005.10.038. PMID 16439210.
• Lehman NL, Verschuren EW, Hsu JY, Cherry AM, Jackson PK (2006). "Overexpression of the anaphase promoting complex/cyclosome inhibitor Emi1 leads to tetraploidy and genomic instability of p53-deficient cells". Cell Cycle 5 (14): 1569–73. doi:10.4161/cc.5.14.2925. PMID 16861914.
• Miller JJ, Summers MK, Hansen DV, Nachury MV, Lehman NL, Loktev A, Jackson PK (2006). "Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor". Genes Dev. 20 (17): 2410–20. doi:10.1101/gad.1454006. PMC 1560415. PMID 16921029.
• Marangos P, Verschuren EW, Chen R, Jackson PK, Carroll J (2007). "Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APCCdh1". J. Cell Biol. 176 (1): 65–75. doi:10.1083/jcb.200607070. PMC 2063628. PMID 17190794.