Fig mosaic virus

Symptoms of fig mosaic on fig leaves
Fig mosaic virus
Virus classification
Group: Group V ((-)ssRNA)
Order: Unassigned
Family: Bunyaviridae
Genus: Emaravirus
Species: Fig mosaic virus

Fig mosaic virus (FMV) is a segmented, negative sense, single-stranded RNA virus that is determined to be the causal agent of fig mosaic disease (FMD) in fig plants, Ficus carica.[1] It is a member of the genus Emaravirus and family Bunyaviridae and is transmitted mainly by the eriophiyd mite Aceria ficus.[2] FMV can cause a range of symptoms varying in severity, including leaf chlorosis, deformity, and mosaic or discoloration patterns, as well as premature fruit drop.[3]

History

Fig mosaic disease (FMD) was first described and suspected to be of viral origin in 1933 by Condit and Horne.[4] It was determined to be of viral origin in 2009 by Jeewan Jyot Walia, Nida M. Salem, and Bryce W. Falk.[5] The disease and associated virus have since been observed in Greece, Italy, Spain, Turkey, Syria, Tunisia, Algeria, Jordan, New Zealand, China, Britain, Puerto Rico, Australia, and the United States.[5]

Classification

FMV has been placed in the genus Emaravirus, which is fairly recently formed and contains other single-stranded, negative-sense, segmented RNA plant viruses. Emaraviruses all contain four to six genome segments. They are also common in their mode of transmission, which is via various species of eriophyid mites by unknown mechanism as well as mechanical modes of transmission.[2] Besides FMV, the genus Emaravirus also includes European mountain ash ringspot-associated virus (EMARaV), rose rosette virus (RRV) and raspberry leaf blotch virus (RLBV).[2]

Genome Structure

The FMV genome consists of segmented (multipartite) negative-sense, single-stranded RNA.[6] The genome has long been thought to have four segments, but recent findings have supported the existence of six RNA genome segments.[7] Each segment has one open reading frame (ORF).[2] The first segment, FMV vcRNA 1 (7093 nt), is common to all viruses of genus Emaravirus and codes for the virus’s 264 kDa RNA dependent RNA polymerase (RdRp), which has endonuclease activity near the N terminus. The second segment, vcRNA2, (2252 nt) encodes a 73 kDa putative glycoprotein. FMV vcRNA3 (1490 nt) encodes a 35 kDa nucleocapsid (N) protein. FMV vcRNA4 (1472 nt) encodes a 40.5 kDa protein with function still unknown.[4] The two most recently discovered segments, RNA5 and RNA6, unlike the other four segments, are not highly conserved within the genus. Perhaps this is because they are truncated or defective, but the functions of the proteins they encode is still undetermined.[7]

Movement between and into cells (FMV-p4)

Like all plant viruses, FMV encodes a movement protein (MP) to enable it to move between cells, usually by increasing the size exclusion limits (SEL) of plasmodesmata to allow viral passage.[8] FMV’s MP is most likely p4, an inference that is supported by its localization in the plasma membrane but has not been definitively shown yet.[2]

Genome replication via cap snatching

FMV vcRNA 1 codes for RNA dependent RNA polymerase protein (RdRp), which has endonuclease function at its N terminus and is packaged in the virion. Once the virion has entered a cell, RdRp binds to host mRNA and uses endonuclease activity to cleave the 5’ methylated cap for use as a primer for mRNA synthesis and as a way to hijack host translational machinery[4]

DMBs and LFPs

Infection with FMV results in distinct double-membrane bodies or particles, called DMBs or DMPs, 90-200 nm in diameter in the cytosol of infected parenchyma cells.[5][6][9] These double membrane-bound bodies are surrounded by a fibril matrix and found only in leaves showing mosaic symptoms at a macro level, never in the uninfected tissue used as a control.[9] Some cultivars have also shown long, flexuous, rod-shaped, virus-like particles (LFPs) in tissues showing signs of necrosis. These are also not seen in uninfected control tissues.[9] Neither type has a determined function.

Symptoms

FMV results in a variety of symptoms, some of which could have strong agricultural detriment, such as decreased fruit yield. Symptoms include appearance of a leaf mosaic pattern, defoliation, decreased fruit yield, vein banding, ringspots, distortion and chlorosis of leaves, and yellow spotting on fruits.[5][7]

Unknowns and Future Research

There are still many aspects of this virus’ molecular structure and function that are unknown. The protein encoded by the fourth segment on the viral genome, RNA4, still has an unknown function.[4] Additionally, the exact molecular mechanisms of FMV’s replication, and Emaravirus replication in general, are still not confirmed.[3] Current research is also working on learning more about the nature of DMBs/DMPs, as they are difficult to isolate from infected plants.[5]

References

  1. ↑ Elbeaino, T; Digiaro, M; Martelli, GP (September 2009). "Complete nucleotide sequence of four RNA segments of fig mosaic virus". Archives of Virology 154: 1719–1727. doi:10.1007/s00705-009-0509-3. PMID 19777155.
  2. 1 2 3 4 5 Ishikawa, K; Maejima, K; Komatsu, K (2013). "Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement". Journal of General Virology 93 (3): 682–686. doi:10.1099/vir.0.047860-0. PMID 23152372.
  3. 1 2 Ishikawa, K; Maejima, K; Nagashima, S (January 2012). "First report of fig mosaic virus infecting common fig (Ficus carica) in Japan". Journal of General Plant Pathology 78: 136–139. doi:10.1007/s10327-012-0359-9.
  4. 1 2 3 4 Walia, JJ; Falk, BW (May 2012). "Fig mosaic virus mRNAs show generation by cap-snatching". Virology 426 (2): 162–166. doi:10.1016/j.virol.2012.01.035. PMID 22356803.
  5. 1 2 3 4 5 Walia, JJ; Salem, NM; Falk, BM (January 2009). "Partial Sequence and Survey Analysis Identify a Multipartite, Negative-Sense RNA Virus Associated with Fig Mosaic". Plant Disease 93 (1): 1–10. doi:10.1094/PDIS-93-1-0004.
  6. 1 2 Elbeaino, T; Digiaro, M; Alabdullah, A (May 2009). "A multipartite single-stranded negative-sense RNA virus is the putative agent of fig mosaic disease". Journal of General Virology 90 (5): 1281–1288. doi:10.1099/vir.0.008649-0. PMID 19264612.
  7. 1 2 3 Ishikawa, K; Maejima, K; Komatsu, K (April 2012). "Identification and characterization of two novel genomic RNA segments of fig mosaic virus, RNA5 and RNA6". Journal of General Virology 93 (7): 1612–1619. doi:10.1099/vir.0.042663-0. PMID 22513386.
  8. ↑ Lazarowitza, SG; Beachy, RN (April 1999). "Viral Movement Proteins as Probes for Intracellular and Intercellular Trafficking in Plants". The Plant Cell 11 (4): 535–548. doi:10.1105/tpc.11.4.535. PMID 10213776.
  9. 1 2 3 Caglayan, K; Medina, V; Gazel, M (October 2009). "Putative agents of fig mosaic disease in Turkey". Turkish Journal of Agriculture & Forestry 33: 469–476. doi:10.3906/tar-0807-20.
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