Fluorescamine

Fluorescamine^[1]

Names
IUPAC name 4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione
Other names Fluram
Identifiers
CAS Number	38183-12-9
ChemSpider	34768
Jmol interactive 3D	Image
MeSH	D005450
PubChem	37927
InChI InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H Key: ZFKJVJIDPQDDFY-UHFFFAOYSA-N
SMILES C1=CC=C(C=C1)C2=COC3(C2=O)C4=CC=CC=C4C(=O)O3
Properties
Chemical formula	C17H10O4
Molar mass	278.26 g/mol
Melting point	153 to 157 °C (307 to 315 °F; 426 to 430 K)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products. It hence has been used as a reagent for the detection of amines and peptides. 1-100 µg of protein and down 10pg protein can be detected.^[2][3] This method is found to suffer of high blanks resulting of high rate of hydrolysis due to used excess concentration. Alternative methods are based on ortho-Phthalaldehyde (OPA), Ellman's reagent (DTNB) and epicocconone.

References

1. Fluram at Sigma-Aldrich
2. Böhlen P., S. Stein,W. Dairman, and S. Undenfriend, Arch. Biochem. Biophys. 155, 1973, 213–220
3. protocol by Fluoprobes