Formaldehyde transketolase

formaldehyde transketolase
Identifiers
EC number	2.2.1.3
CAS number	76774-46-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a formaldehyde transketolase (EC 2.2.1.3) is an enzyme that catalyzes the chemical reaction

D-xylulose 5-phosphate + formaldehyde $\rightleftharpoons$ glyceraldehyde 3-phosphate + glycerone

Thus, the two substrates of this enzyme are D-xylulose 5-phosphate and formaldehyde, whereas its two products are glyceraldehyde 3-phosphate and glycerone.

This enzyme belongs to the family of transferases, specifically those transferring aldehyde or ketonic groups (transaldolases and transketolases, respectively). The systematic name of this enzyme class is D-xylulose-5-phosphate:formaldehyde glycolaldehydetransferase. This enzyme is also called dihydroxyacetone synthase. This enzyme participates in methane metabolism. It employs one cofactor, thiamin diphosphate.

References

• Bystrykh, L. V.; A. P. Sokolov; Iu. A. Trotsenko (1981). "Separation of transketolase and dihydroxyacetone synthase from methylotrophic yeasts" [Separation of transketolase and dihydroxyacetone synthase from methylotrophic yeasts]. Doklady Akademii Nauk SSSR (in Russian) 258 (2): 499–501. PMID 7249920.
• Kato N, Higuchi T, Sakazawa C, Nishizawa T, Tani Y, Yamada H (1982). "Purification and properties of a transketolase responsible for formaldehyde fixation in a methanol-utilizing yeast, candida boidinii (Kloeckera sp.) No. 2201". Biochim. Biophys. Acta 715 (2): 143–50. doi:10.1016/0304-4165(82)90352-x. PMID 7074134.
• Waites MJ, Quayle JR (1981). "The interrelation transketolase and dihydroxyacetone synthase activities in the methylotrophic yeast Candida boidinii". J. Gen. Microbiol. 124 (2): 309–16. doi:10.1099/00221287-124-2-309. PMID 6276498.