G3BP1

GTPase activating protein (SH3 domain) binding protein 1
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3Q90​, 4FCJ​, 4FCM​, 4IIA​
Identifiers
Symbols	G3BP1 ; G3BP; HDH-VIII
External IDs	OMIM: 608431 MGI: 1351465 HomoloGene: 38096 GeneCards: G3BP1 Gene
EC number	3.6.4.12, 3.6.4.13
Gene ontology Molecular function • DNA binding • mRNA binding • ATP-dependent DNA helicase activity • ATP-dependent RNA helicase activity • endonuclease activity • protein binding • ATP binding • poly(A) RNA binding Cellular component • nucleus • cytoplasm • cytosol • plasma membrane • focal adhesion • cytoplasmic stress granule Biological process • transport • Ras protein signal transduction • DNA duplex unwinding • negative regulation of canonical Wnt signaling pathway • nucleic acid phosphodiester bond hydrolysis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10146	27041
Ensembl	ENSG00000145907	ENSMUSG00000018583
UniProt	Q13283	P97855
RefSeq (mRNA)	NM_005754	NM_013716
RefSeq (protein)	NP_005745	NP_038744
Location (UCSC)	Chr 5: 151.77 – 151.81 Mb	Chr 11: 55.47 – 55.5 Mb
PubMed search

Ras GTPase-activating protein-binding protein 1 is an enzyme that in humans is encoded by the G3BP1 gene.^[1][2][3]

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It was originally reported to bind specifically to the Ras-GTPase-activating protein by associating with its SH3 domain, but this interaction has recently been challenged.^[4] Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.^[3]

Interactions

G3BP1 has been shown to interact with USP10.^[5] It also interacts with SND1[5].

References

1. Parker F, Maurier F, Delumeau I, Duchesne M, Faucher D, Debussche L, Dugue A, Schweighoffer F, Tocque B (Jul 1996). "A Ras-GTPase-activating protein SH3-domain-binding protein". Mol Cell Biol 16 (6): 2561–9. PMC 231246. PMID 8649363.
2. Costa M, Ochem A, Staub A, Falaschi A (Mar 1999). "Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the ras transduction pathway". Nucleic Acids Res 27 (3): 817–21. doi:10.1093/nar/27.3.817. PMC 148252. PMID 9889278.
3. "Entrez Gene: G3BP1 GTPase activating protein (SH3 domain) binding protein 1".
4. Annibaldi, A; Dousse, A; Martin, S; Tazi, J; Widmann, C (2011). "Revisiting G3BP1 as a RasGAP binding protein: Sensitization of tumor cells to chemotherapy by the RasGAP 317-326 sequence does not involve G3BP1". PLoS ONE 6 (12): e29024. doi:10.1371/journal.pone.0029024. PMC 3242762. PMID 22205990.
5. Soncini, C; Berdo I; Draetta G (Jun 2001). "Ras-GAP SH3 domain binding protein (G3BP) is a modulator of USP10, a novel human ubiquitin specific protease". Oncogene (England) 20 (29): 3869–79. doi:10.1038/sj.onc.1204553. ISSN 0950-9232. PMID 11439350.

[5]Gao X, Ge L, Shao J, Su C, Zhao H, Saarikettu J, Yao X, Yao Z, Silvennoinen O, Yang J., Tudor-SN interacts with and co-localizes with G3BP in stress granules under stress conditions.FEBS Letters, Volume 584, Issue 16, Pages 3525-3532.

Further reading

• Irvine K, Stirling R, Hume D, Kennedy D (2005). "Rasputin, more promiscuous than ever: a review of G3BP". Int. J. Dev. Biol. 48 (10): 1065–77. doi:10.1387/ijdb.041893ki. PMID 15602692.
• Onno M, Nakamura T, Hillova J, Hill M (1993). "Rearrangement of the human tre oncogene by homologous recombination between Alu repeats of nucleotide sequences from two different chromosomes". Oncogene 7 (12): 2519–23. PMID 1461655.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K; et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Gallouzi IE, Parker F, Chebli K; et al. (1998). "A Novel Phosphorylation-Dependent RNase Activity of GAP-SH3 Binding Protein: a Potential Link between Signal Transduction and RNA Stability". Mol. Cell. Biol. 18 (7): 3956–65. PMC 108980. PMID 9632780.
• Kociok N, Esser P, Unfried K; et al. (1999). "Upregulation of the RAS-GTPase activating protein (GAP)-binding protein (G3BP) in proliferating RPE cells". J. Cell. Biochem. 74 (2): 194–201. doi:10.1002/(SICI)1097-4644(19990801)74:2<194::AID-JCB5>3.0.CO;2-M. PMID 10404389.
• Soncini C, Berdo I, Draetta G (2001). "Ras-GAP SH3 domain binding protein (G3BP) is a modulator of USP10, a novel human ubiquitin specific protease". Oncogene 20 (29): 3869–79. doi:10.1038/sj.onc.1204553. PMID 11439350.
• Tourrière H, Gallouzi IE, Chebli K; et al. (2001). "RasGAP-Associated Endoribonuclease G3BP: Selective RNA Degradation and Phosphorylation-Dependent Localization". Mol. Cell. Biol. 21 (22): 7747–60. doi:10.1128/MCB.21.22.7747-7760.2001. PMC 99945. PMID 11604510.
• Barnes CJ, Li F, Mandal M; et al. (2002). "Heregulin induces expression, ATPase activity, and nuclear localization of G3BP, a Ras signaling component, in human breast tumors". Cancer Res. 62 (5): 1251–5. PMID 11888885.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Tourrière H, Chebli K, Zekri L; et al. (2003). "The RasGAP-associated endoribonuclease G3BP assembles stress granules". J. Cell Biol. 160 (6): 823–31. doi:10.1083/jcb.200212128. PMC 2173781. PMID 12642610.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Brill LM, Salomon AR, Ficarro SB; et al. (2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry". Anal. Chem. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID 15144186.
• Beausoleil SA, Jedrychowski M, Schwartz D; et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Katsafanas GC, Moss B (2005). "Vaccinia virus intermediate stage transcription is complemented by Ras-GTPase-activating protein SH3 domain-binding protein (G3BP) and cytoplasmic activation/proliferation-associated protein (p137) individually or as a heterodimer". J. Biol. Chem. 279 (50): 52210–7. doi:10.1074/jbc.M411033200. PMID 15471883.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Ong SE, Mittler G, Mann M (2005). "Identifying and quantifying in vivo methylation sites by heavy methyl SILAC". Nat. Methods 1 (2): 119–26. doi:10.1038/nmeth715. PMID 15782174.

External links

• G3BP1 human gene location in the UCSC Genome Browser.
• G3BP1 human gene details in the UCSC Genome Browser.