Enterobacteria phage G4

Enterobacteria phage G4
Virus classification
Group: Group II (ssDNA)
Family: Microviridae
Genus: Microvirus
Species: Enterobacteria phage G4

Enterobacteria phage G4 is a bacteriophage capable of infecting susceptible bacterial cells. The phage was originally isolated from samples of raw sewage and infects E. coli.[1] Its isometric capsid contains a single-stranded circular genome of about 5.5 kbp. Phage G4 bears high genetic and structural similarity to the phage ΦX174, though the two phage genomes are up to 39 percent different by sequence.[2]

Synthetic G4

G4 has been synthesized.

Full-sized genomes of bacteriophage G4 molecules were completed from short overlapping synthetic oligonucleotides by direct assembly polymerase chain reaction and ligase chain reaction followed by fusion polymerase chain reaction with flanking primers.

The synthetic phage G4 genomes, after transformation into E. coli, were translated by the host cells and led to complete and infective G4 viral particles.[3]

References

  1. ↑ Godson, G (1 March 1974). "Evolution of φX174. Isolation of four new φX-like phages and comparison with φX174". Virology 58 (1): 272–289. doi:10.1016/0042-6822(74)90161-5.
  2. ↑ Godson, G. N.; Barrell, B. G.; Staden, R.; Fiddes, J. C. (16 November 1978). "Nucleotide sequence of bacteriophage G4 DNA". Nature 276 (5685): 236–247. doi:10.1038/276236a0.
  3. ↑ Yang, Ruilin; Han, Yonghua; Ye, Yiwang; Liu, Yuchen; Jiang, Zhimao; Gui, Yaoting; Cai, Zhiming; Wrede, Paul (16 November 2011). "Chemical Synthesis of Bacteriophage G4". PLoS ONE 6 (11): e27062. doi:10.1371/journal.pone.0027062.

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