GGA2

Golgi-associated, gamma adaptin ear containing, ARF binding protein 2
PDB rendering based on 1mhq.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1MHQ​
Identifiers
Symbols	GGA2 ; VEAR
External IDs	OMIM: 606005 MGI: 1921355 HomoloGene: 22860 GeneCards: GGA2 Gene
Gene ontology Molecular function • protein binding • ADP-ribosylation factor binding Cellular component • Golgi apparatus • trans-Golgi network • endosome membrane • clathrin adaptor complex • clathrin-coated vesicle Biological process • intracellular protein transport • vesicle-mediated transport Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	23062	74105
Ensembl	ENSG00000103365	ENSMUSG00000030872
UniProt	Q9UJY4	Q6P5E6
RefSeq (mRNA)	NM_015044	NM_028758
RefSeq (protein)	NP_055859	NP_083034
Location (UCSC)	Chr 16: 23.46 – 23.52 Mb	Chr 7: 121.99 – 122.02 Mb
PubMed search

ADP-ribosylation factor-binding protein GGA2 is a protein that in humans is encoded by the GGA2 gene.^[1][2][3]

Function

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.^[3]

Interactions

GGA2 has been shown to interact with RABEP1,^[4] Sortilin 1,^[5][6] BACE2^[7] and CLINT1.^[8][9]

References

1. Hirst J, Lui WW, Bright NA, Totty N, Seaman MN, Robinson MS (Apr 2000). "A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome". The Journal of Cell Biology 149 (1): 67–80. doi:10.1083/jcb.149.1.67. PMC 2175106. PMID 10747088.
2. Boman AL, Zhang CJ, Zhu X, Kahn RA (Apr 2000). "A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi". Molecular Biology of the Cell 11 (4): 1241–55. doi:10.1091/mbc.11.4.1241. PMC 14844. PMID 10749927.
3. "Entrez Gene: GGA2 golgi associated, gamma adaptin ear containing, ARF binding protein 2".
4. Mattera R, Arighi CN, Lodge R, Zerial M, Bonifacino JS (Jan 2003). "Divalent interaction of the GGAs with the Rabaptin-5-Rabex-5 complex". The EMBO Journal 22 (1): 78–88. doi:10.1093/emboj/cdg015. PMC 140067. PMID 12505986.
5. Nielsen MS, Madsen P, Christensen EI, Nykjaer A, Gliemann J, Kasper D, Pohlmann R, Petersen CM (May 2001). "The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein". The EMBO Journal 20 (9): 2180–90. doi:10.1093/emboj/20.9.2180. PMC 125444. PMID 11331584.
6. Jacobsen L, Madsen P, Nielsen MS, Geraerts WP, Gliemann J, Smit AB, Petersen CM (Jan 2002). "The sorLA cytoplasmic domain interacts with GGA1 and -2 and defines minimum requirements for GGA binding". FEBS Letters 511 (1-3): 155–8. doi:10.1016/S0014-5793(01)03299-9. PMID 11821067.
7. He X, Chang WP, Koelsch G, Tang J (Jul 2002). "Memapsin 2 (beta-secretase) cytosolic domain binds to the VHS domains of GGA1 and GGA2: implications on the endocytosis mechanism of memapsin 2". FEBS Letters 524 (1-3): 183–7. doi:10.1016/S0014-5793(02)03052-1. PMID 12135764.
8. Wasiak S, Legendre-Guillemin V, Puertollano R, Blondeau F, Girard M, de Heuvel E, Boismenu D, Bell AW, Bonifacino JS, McPherson PS (Sep 2002). "Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics". The Journal of Cell Biology 158 (5): 855–62. doi:10.1083/jcb.200205078. PMC 2173151. PMID 12213833.
9. Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell EJ (Nov 2002). "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi". Molecular Biology of the Cell 13 (11): 4060–73. doi:10.1091/mbc.E02-03-0171. PMC 133614. PMID 12429846.

Further reading

• Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Kikuno R, Nagase T, Ishikawa K, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Jun 1999). "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research 6 (3): 197–205. doi:10.1093/dnares/6.3.197. PMID 10470851.
• Loftus BJ, Kim UJ, Sneddon VP, Kalush F, Brandon R, Fuhrmann J, Mason T, Crosby ML, Barnstead M, Cronin L, Deslattes Mays A, Cao Y, Xu RX, Kang HL, Mitchell S, Eichler EE, Harris PC, Venter JC, Adams MD (Sep 1999). "Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q". Genomics 60 (3): 295–308. doi:10.1006/geno.1999.5927. PMID 10493829.
• Poussu A, Lohi O, Lehto VP (Mar 2000). "Vear, a novel Golgi-associated protein with VHS and gamma-adaptin "ear" domains". The Journal of Biological Chemistry 275 (10): 7176–83. doi:10.1074/jbc.275.10.7176. PMID 10702286.
• Dell'Angelica EC, Puertollano R, Mullins C, Aguilar RC, Vargas JD, Hartnell LM, Bonifacino JS (Apr 2000). "GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex". The Journal of Cell Biology 149 (1): 81–94. doi:10.1083/jcb.149.1.81. PMC 2175099. PMID 10747089.
• Takatsu H, Yoshino K, Nakayama K (May 2000). "Adaptor gamma ear homology domain conserved in gamma-adaptin and GGA proteins that interact with gamma-synergin". Biochemical and Biophysical Research Communications 271 (3): 719–25. doi:10.1006/bbrc.2000.2700. PMID 10814529.
• Puertollano R, Randazzo PA, Presley JF, Hartnell LM, Bonifacino JS (Apr 2001). "The GGAs promote ARF-dependent recruitment of clathrin to the TGN". Cell 105 (1): 93–102. doi:10.1016/S0092-8674(01)00299-9. PMID 11301005.
• Nielsen MS, Madsen P, Christensen EI, Nykjaer A, Gliemann J, Kasper D, Pohlmann R, Petersen CM (May 2001). "The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein". The EMBO Journal 20 (9): 2180–90. doi:10.1093/emboj/20.9.2180. PMC 125444. PMID 11331584.
• Puertollano R, Aguilar RC, Gorshkova I, Crouch RJ, Bonifacino JS (Jun 2001). "Sorting of mannose 6-phosphate receptors mediated by the GGAs". Science 292 (5522): 1712–6. doi:10.1126/science.1060750. PMID 11387475.
• Zhu Y, Doray B, Poussu A, Lehto VP, Kornfeld S (Jun 2001). "Binding of GGA2 to the lysosomal enzyme sorting motif of the mannose 6-phosphate receptor". Science 292 (5522): 1716–8. doi:10.1126/science.1060896. PMID 11387476.
• Takatsu H, Katoh Y, Shiba Y, Nakayama K (Jul 2001). "Golgi-localizing, gamma-adaptin ear homology domain, ADP-ribosylation factor-binding (GGA) proteins interact with acidic dileucine sequences within the cytoplasmic domains of sorting receptors through their Vps27p/Hrs/STAM (VHS) domains". The Journal of Biological Chemistry 276 (30): 28541–5. doi:10.1074/jbc.C100218200. PMID 11390366.
• Jacobsen L, Madsen P, Nielsen MS, Geraerts WP, Gliemann J, Smit AB, Petersen CM (Jan 2002). "The sorLA cytoplasmic domain interacts with GGA1 and -2 and defines minimum requirements for GGA binding". FEBS Letters 511 (1-3): 155–8. doi:10.1016/S0014-5793(01)03299-9. PMID 11821067.
• Doray B, Bruns K, Ghosh P, Kornfeld S (May 2002). "Interaction of the cation-dependent mannose 6-phosphate receptor with GGA proteins". The Journal of Biological Chemistry 277 (21): 18477–82. doi:10.1074/jbc.M201879200. PMID 11886874.
• Takatsu H, Yoshino K, Toda K, Nakayama K (Jul 2002). "GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors". The Biochemical Journal 365 (Pt 2): 369–78. doi:10.1042/BJ20020428. PMC 1222692. PMID 11950392.
• He X, Chang WP, Koelsch G, Tang J (Jul 2002). "Memapsin 2 (beta-secretase) cytosolic domain binds to the VHS domains of GGA1 and GGA2: implications on the endocytosis mechanism of memapsin 2". FEBS Letters 524 (1-3): 183–7. doi:10.1016/S0014-5793(02)03052-1. PMID 12135764.
• Wasiak S, Legendre-Guillemin V, Puertollano R, Blondeau F, Girard M, de Heuvel E, Boismenu D, Bell AW, Bonifacino JS, McPherson PS (Sep 2002). "Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics". The Journal of Cell Biology 158 (5): 855–62. doi:10.1083/jcb.200205078. PMC 2173151. PMID 12213833.
• Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell EJ (Nov 2002). "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi". Molecular Biology of the Cell 13 (11): 4060–73. doi:10.1091/mbc.E02-03-0171. PMC 133614. PMID 12429846.